Polr1e (BC034783) Mouse Tagged ORF Clone

CAT#: MR207725

  • TrueORF®

Polr1e (Myc-DDK-tagged) - Mouse polymerase (RNA) I polypeptide E (cDNA clone MGC:35831 IMAGE:4022372)


  "BC034783" in other vectors (4)


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Reconstitution Protocol

USD 290.00

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Size
    • 10 ug

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Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Symbol Polr1e
Synonyms 53kDa
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR207725 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGCCCTGGAGTCTCCTGGGATGGATGACCAGGCTGGAGACACGGAGACAGAGGCGCTACAGAGTG
CAAGGTGGCTGTACTGTGGAGAGCCCGACGACAGGCAGAAAGCTGTGCTGGTCCAGTTCTCCAATGGGAA
GCTGCAGAATCCGGGCGACATGCGCTTCACCTTGTACAACAGCACCGACTTAGTAAACCCCAGGCAGAGG
AGTCACCGGATCGTGGCAGCTGAAACAGACAGGCTCTCCTATGTCGGAAACAATTTTGGGACCGGTGCCC
TCAAATGCAATGCTCTTTGCAGACACTTTGTGGGAATTCTGAATAAGACCTCTGGCCAGATGGAAGTCTA
TGATGCCGAACTGTTCAACATGCAGCCGCTGTTTGCTGGAATGGGAACTGAAGTCATCAAACTTGGTGGC
CAACACCTTTACCTGCTGGCCTTCTGCCAACCCTCCAAGAACCTGGCTGAGGCAGGTGACCTGCTGCTGA
GCCGACACCGGCAAGGCCATTGCATTGCAGTCCTGCTGGATGACGATGCTATTGAGCGAGAGCCTCCTCT
GGAGAATCAGAACAAGACTTTCAGGGACAAGTTGGATTCTTGCATTGAAGCCTTTGGAAGCACCAAGCAG
AAGCGGTCCCTAAACTCCAGGCGGATGAACAAAGTTGGCAGCGAGTCTCTGAATCTCAGTGTGGCGAAAG
CCGCGGAGAGTATCATTGATACAAAGGGTGTGAATGCGCTGGTCAGTGATGCCATGCAGGATGACTTACA
ATATGACGCCCTCTACCTTCCTCCCTGCTACGCTGACGCAGCCAAGCCGGAAGACGTGTATAGATTTGAA
GACATTCTGTCCCCGGCCGAGTATGATGCTCTTGAGAGCCCATCCGAAGCCTTCAGAAAGGTCACATCGG
AAGACATACTGAAGATGATTGAAGAGAACAGCCACTGCTCCTACGTCATAGAAATGCTGAAGTCTCTGCC
CATAGATGAGGTCCACCGGAACCGGCAAGCCCGGAGCATCTGGTTCCTAGACGCCCTCATCAGATTCCGA
GCTCAGAAAGTGATAAAGGGGAAAAGAGCCTTAGGGCCTGGAATCCCGCACATTATCAACACCAAGCTGC
TGAAACAGTTCACCTGCTTGACCTACAACAACGGCAGGTTACAGAACCTAATTTCAAGTTCTATGAGGGC
CAAGATCACTTCGTATGCGATCATACTTGCCCTGCACATAAATAACTTCCAGGTTGACCTGACAGCGTTG
CAGAAGGACTTGAAGCTGAGTGAGAAAAGGATTATTGAGATAGCGAAAGCCATGAGGCTGAAGATCTCCA
AACAAAAGGTGTCCCTGGCAGATGGCAGGGAAGAGAGTCACAGACTGGGCACTCTGTCTGTCCCACTGCC
TCCAGCCCAGAACTCTGATCGACAGTCAAAGCGGAGGAAAATGAAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR207725 protein sequence
Red=Cloning site Green=Tags(s)

MAALESPGMDDQAGDTETEALQSARWLYCGEPDDRQKAVLVQFSNGKLQNPGDMRFTLYNSTDLVNPRQR
SHRIVAAETDRLSYVGNNFGTGALKCNALCRHFVGILNKTSGQMEVYDAELFNMQPLFAGMGTEVIKLGG
QHLYLLAFCQPSKNLAEAGDLLLSRHRQGHCIAVLLDDDAIEREPPLENQNKTFRDKLDSCIEAFGSTKQ
KRSLNSRRMNKVGSESLNLSVAKAAESIIDTKGVNALVSDAMQDDLQYDALYLPPCYADAAKPEDVYRFE
DILSPAEYDALESPSEAFRKVTSEDILKMIEENSHCSYVIEMLKSLPIDEVHRNRQARSIWFLDALIRFR
AQKVIKGKRALGPGIPHIINTKLLKQFTCLTYNNGRLQNLISSSMRAKITSYAIILALHINNFQVDLTAL
QKDLKLSEKRIIEIAKAMRLKISKQKVSLADGREESHRLGTLSVPLPPAQNSDRQSKRRKMN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC034783
ORF Size 1446 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq BC034783, AAH34783
RefSeq Size 1757 bp
RefSeq ORF 1448 bp
Locus ID 64424
MW 54.1 kDa

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