Net1 (BC004699) Mouse Tagged ORF Clone

CAT#: MG208638

Net1 (GFP-tagged) - Mouse neuroepithelial cell transforming gene 1 (cDNA clone MGC:5716 IMAGE:3499258)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Net1
• Synonyms: Net1a, mNET1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG208638 representing BC004699
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGGCGCATGATGAGATCGGAGGTCTCCTGCCCATTAAAAGGACGATCCGAGTCTTAGATGTTAATA
  ACCAGCCCTTCCGGGAGCAGGAGGAGCCAAGCAATAAGAGAGTTCGGCCTCTAGCTCGGGTCACATCTTT
  GGCAAACTTAATCTCTCCCGTAAGAAATGGAGCAGTCAGGCGCTTCGGTCAGACCATACAGTCGTTTACC
  CTTCGTGGTGACCACAGATCCCCAGCCTCTGCCCAGAAGTCATTCAGCAGGTCCACCGTCCCCACACCCA
  CCAAGAGAAGAAGCAGTGCACTATGGTCGGAAATGCTAGACATCAATATGAAGGAGTCCTTGACCACTCG
  AGAGATCAAACGTCAGGAGGCCATATATGAATTGTCCCGAGGGGAACAGGACTTGATTGAGGATCTCAAG
  CTCGCTAGAAAGGCCTACCATGACCCCATGTTAAAGCTGTCTATCATGTCAGAAGAGGAACTCACGCACA
  TATTTGGAGACTTGGATGCTTATATACCTCTGCACGAAGATCTGTTAGCAAGAATTGGAGAAGCAACCAA
  GCCTGATGGGACTGTGGAGCAGATCGGTCACATTCTCGTGAACTGGTTACCAGGCCTGAATGCCTACAGA
  GGCTACTGCAGTAACCAGCTGGCGGCCAAGGCTCTTCTGGATCAGAAGAAACAAGACCCAAGAGTCCAAG
  ACTTCCTCCAGCGCTGTCTGGAGTCTCCTTTCAGTCGAAAACTAGATCTTTGGAGTTTCCTAGATATCCC
  TCGAAGTCGCCTCGTCAAGTACCCCTTACTGTTAAAAGAAATCCTTAGACACACTCCCAAAGACCACCGT
  GATGTTCAGCTTCTAGAAGAGGCAATATTGATAATACAAGGAGTTCTTTCTGATATCAACTTGAAGAAAG
  GCGAATCTGAGTGTCAGTATTACATCAACAAGCTGGAGTACCTGGACGAGAAGCAGAAGGACCCTAGGAT
  CGAAGCGAGCAAAGTGCTGCTGTGCCATGGGGAGCTGAAGAATAAAAGCGGCCATAAACTGTACATTTTC
  CTATTTCAAGACATCTTGGTTTTGACTCGGCCTGTCACCCGAAATGAGCGCCACTTGTACCAGGTTTACC
  GGCAGCCCATCCCAGTCCAGGAGCTGGTGCTGGAAGACCTGCAGGACGGGGATGTGCGCATGGGTGGCTC
  CTTCCGAGGGGCCTTCGGCAACTCAGACAAAGCTAAAAATATCTTTAGGGTCCGCTTCCAAGACCCTTCT
  CCAGGCCACTCCCACACACTGCAAGCCAATGATGTGTTCCATAAGCAGCAGTGGTTCAACTGTATTCGCG
  CGGCCATTGCCCCCTTCCAGCGGGCCGCCAGCCCCTTAGAGCTTCAGGGCTTGCCAGACCTCCACGAAGA
  GTGTGAAGAGAACAACCCTTCTGCTGGGAACCTCAGAGCCCAGCGACGGTCATGCGTGGTTCCTGGTGTC
  ATGCAGATAGACGAGGAAAGCGCCCTGGATTGTGGCTCCAGTGTACAGACAGTGGAAGACACCAGGAACA
  TGAAGGCACAGCGGCCTCAGCCTGGCTTACGAAGAGCAAGGGACAAAGCACAGTCAGGTGGCAAAAAGAA
  AGAGACTTTGGTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG208638 representing BC004699
  Red=Cloning site Green=Tags(s)
  
  MVAHDEIGGLLPIKRTIRVLDVNNQPFREQEEPSNKRVRPLARVTSLANLISPVRNGAVRRFGQTIQSFT
  LRGDHRSPASAQKSFSRSTVPTPTKRRSSALWSEMLDINMKESLTTREIKRQEAIYELSRGEQDLIEDLK
  LARKAYHDPMLKLSIMSEEELTHIFGDLDAYIPLHEDLLARIGEATKPDGTVEQIGHILVNWLPGLNAYR
  GYCSNQLAAKALLDQKKQDPRVQDFLQRCLESPFSRKLDLWSFLDIPRSRLVKYPLLLKEILRHTPKDHR
  DVQLLEEAILIIQGVLSDINLKKGESECQYYINKLEYLDEKQKDPRIEASKVLLCHGELKNKSGHKLYIF
  LFQDILVLTRPVTRNERHLYQVYRQPIPVQELVLEDLQDGDVRMGGSFRGAFGNSDKAKNIFRVRFQDPS
  PGHSHTLQANDVFHKQQWFNCIRAAIAPFQRAASPLELQGLPDLHEECEENNPSAGNLRAQRRSCVVPGV
  MQIDEESALDCGSSVQTVEDTRNMKAQRPQPGLRRARDKAQSGGKKKETLV
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC004699
• ORF Size: 1625 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC004699, AAH04699
• RefSeq Size: 3313
• RefSeq ORF: 1625
• Locus ID: 56349