Xpc (NM_009531) Mouse Tagged ORF Clone

CAT#: MG211103

Xpc (GFP-tagged) - Mouse xeroderma pigmentosum, complementation group C (Xpc)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Xpc
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG211103 representing NM_009531
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCCAAAGCGCACCGCAGACGGAAGGCGGCGGAAGCGGGGCCAGAAAACCGAGGACAACAAAGTAG
  CCCGGCACGAGGAGAGCGTTGCGGATGACTTTGAAGATGAGAAGCAGAAACCCCGAAGGAAGAGCTCTTT
  CCCGAAAGTCTCTCAAGGAAAGAGGAAGAGGGGCTGCAGTGATCCAGGGGACCCCACAAATGGTGCAGCA
  AAAAAGAAAGTGGCCAAAGCCACTGCTAAATCCAAGAATCTCAAGGTTCTGAAGGAGGAAGCACTCAGCG
  ACGGGGATGACTTCCGGGACTCACCAGCTGACTGCAAGAAGGCAAAGAAACACCCAAAAAGCAAGGTGGT
  GGACCAAGGCACTGATGAAGATGACAGTGAGGATGACTGGGAGGAGGTGGAAGAGCTTACTGAACCTGTG
  CTGGACATGGGAGAAAATTCTGCCACCTCACCGTCTGACATGCCTGTGAAGGCGGTGGAGATTGAAATTG
  AAACACCACAGCAGGCGAAAGAACGGGAAAGAAGTGAGAAGATAAAGATGGAGTTTGAGACATACCTGCG
  GAGGATGATGAAGCGTTTCAATAAAGAAGTACAGGAGAACATGCACAAGGTTCACCTGCTGTGCCTGCTG
  GCCAGTGGCTTCTATCGAAATAGCATCTGCCGACAGCCAGATCTGCTGGCCATTGGCCTCTCCATCATTC
  CAATTCGCTTTACCAAGGTGCCACTTCAAGATAGGGATGCCTACTACCTTTCAAACCTGGTAAAGTGGTT
  CATCGGAACCTTCACTGTCAACGCTGACCTTTCAGCCAGCGAGCAGGACGACCTGCAGACCACCTTGGAA
  AGGAGGATTGCCATTTACTCTGCGAGGGATAATGAAGAGTTGGTCCATATATTTCTTCTGATTCTTCGGG
  CTCTGCAGCTGCTCACCCGGCTGGTCTTGTCTCTGCAGCCCATTCCACTGAAGTCAGCTGTGACAAAGGG
  GAGGAAATCTTCCAAGGAGACATCTGTAGAGGGTCCTGGAGGTTCTTCAGAACTCTCTAGTAACAGTCCA
  GAAAGCCACAACAAACCTACGACCAGCAGGAGAATCAAAGAAGAAGAAACCTTGTCTGAGGGCAGAGGCA
  AAGCAACCGCCAGGGGGAAGAGAGGCACAGGCACTGCGGGCAGCAGGCAGCGGAGGAAGCCCTCTTGCAG
  CGAGGGAGAGGAGGCCGAGCAGAAAGTCCAGGGCCGTCCACATGCCCGGAAGCGGCGTGTGGCTGCCAAG
  GTGTCATACAAAGAGGAGAGTGAGAGCGATGGGGCAGGCAGCGGCTCTGACTTTGAACCGTCCAGTGGGG
  AGGGCCAGCATTCCTCTGATGAGGATTGTGAGCCTGGCCCTCGCAAGCAGAAGAGGGCCTCAGCTCCTCA
  GAGGACAAAGGCTGGGTCTAAGAGTGCTTCCAAGACCCAACGCGGAAGCCAGTGTGACCCGTCAAGCTTT
  CCGGAGGCGTCTTCAAGCTCTTCAGGCTGTAAGAGAGGCAAGAAGGTTTCCAGTGGTGCTGAAGAGATGG
  CAGATAGGAAACCTGCTGGTGTAGACCAGTGGCTGGAGGTGTACTGTGAGCCACAGGCAAAGTGGGTGTG
  TGTGGACTGTGTACATGGTGTGGTGGGCCAGCCTGTGGCCTGTTACAAATATGCCACCAAACCCATGACC
  TATGTTGTAGGCATTGACAGTGATGGCTGGGTCCGAGATGTTACTCAGAGGTATGACCCAGCCTGGATGA
  CCGCAACCCGCAAGTGCCGGGTTGATGCTGAGTGGTGGGCTGAGACCTTGAGACCCTATCGGAGCCTACT
  TACGGAGAGGGAGAAGAAGGAAGACCAGGAGTTTCAGGCGAAGCACCTGGACCAGCCTTTGCCCACCTCC
  ATCAGCACATACAAGAACCACCCTCTGTATGCCCTGAAGCGCCACCTCTTGAAATTCCAGGCTATCTACC
  CTGAGACAGCTGCGGTGCTCGGGTATTGTCGTGGAGAAGCAGTCTATTCCAGGGATTGCGTGCATACCTT
  GCACTCCAGGGACACATGGCTGAAGCAAGCAAGAGTGGTGCGGCTTGGAGAAGTGCCCTATAAGATGGTG
  AAAGGCTTCTCTAACCGTGCCCGGAAAGCCCGGCTCTCGGAGCCCCAGCTGCATGACCACAACGACTTGG
  GCCTCTATGGTCACTGGCAGACGGAAGAGTATCAGCCTCCTATAGCTGTCGATGGGAAGGTGCCTCGGAA
  TGAGTTTGGGAACGTGTACCTCTTCCTGCCCAGCATGATGCCCGTCGGCTGTGTCCAGATGACCCTGCCT
  AACCTGAACCGGGTGGCACGCAAGCTGGGCATCGACTGCGTGCAGGCCATCACTGGCTTCGATTTCCATG
  GAGGCTATTGCCATCCAGTAACTGATGGCTACATTGTCTGTGAGGAATTCAGAGACGTGCTGCTGGCTGC
  TTGGGAGAATGAACAGGCCATCATTGAAAAGAAGGAGAAGGAGAAAAAGGAAAAGCGGGCGCTGGGGAAC
  TGGAAGCTGCTGGTCATAGGACTCCTCATCAGAGAGAGGCTGAAACTCCGCTATGGGGCCAAGAGCGAGG
  CAGCAGCTCCCCATGCTGCTGGAGGCGGACTCTCTTCTGATGAAGAGGAAGGGACCAGTTCACAAGCAGA
  AGCAGCCAGAGTCCTGGCTGCCTCCTGGCCACAGAACCGAGAGGATCCAGAACAGAAGTCCGAGTACACT
  AAGATGACTCGGAAGAGGCGGGCGGCAGAGGCTTCCCACCTCTTTCCGTTTGAGAAGCTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG211103 representing NM_009531
  Red=Cloning site Green=Tags(s)
  
  MAPKRTADGRRRKRGQKTEDNKVARHEESVADDFEDEKQKPRRKSSFPKVSQGKRKRGCSDPGDPTNGAA
  KKKVAKATAKSKNLKVLKEEALSDGDDFRDSPADCKKAKKHPKSKVVDQGTDEDDSEDDWEEVEELTEPV
  LDMGENSATSPSDMPVKAVEIEIETPQQAKERERSEKIKMEFETYLRRMMKRFNKEVQENMHKVHLLCLL
  ASGFYRNSICRQPDLLAIGLSIIPIRFTKVPLQDRDAYYLSNLVKWFIGTFTVNADLSASEQDDLQTTLE
  RRIAIYSARDNEELVHIFLLILRALQLLTRLVLSLQPIPLKSAVTKGRKSSKETSVEGPGGSSELSSNSP
  ESHNKPTTSRRIKEEETLSEGRGKATARGKRGTGTAGSRQRRKPSCSEGEEAEQKVQGRPHARKRRVAAK
  VSYKEESESDGAGSGSDFEPSSGEGQHSSDEDCEPGPRKQKRASAPQRTKAGSKSASKTQRGSQCDPSSF
  PEASSSSSGCKRGKKVSSGAEEMADRKPAGVDQWLEVYCEPQAKWVCVDCVHGVVGQPVACYKYATKPMT
  YVVGIDSDGWVRDVTQRYDPAWMTATRKCRVDAEWWAETLRPYRSLLTEREKKEDQEFQAKHLDQPLPTS
  ISTYKNHPLYALKRHLLKFQAIYPETAAVLGYCRGEAVYSRDCVHTLHSRDTWLKQARVVRLGEVPYKMV
  KGFSNRARKARLSEPQLHDHNDLGLYGHWQTEEYQPPIAVDGKVPRNEFGNVYLFLPSMMPVGCVQMTLP
  NLNRVARKLGIDCVQAITGFDFHGGYCHPVTDGYIVCEEFRDVLLAAWENEQAIIEKKEKEKKEKRALGN
  WKLLVIGLLIRERLKLRYGAKSEAAAPHAAGGGLSSDEEEGTSSQAEAARVLAASWPQNREDPEQKSEYT
  KMTRKRRAAEASHLFPFEKL
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009531
• ORF Size: 2790 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_009531.2, NP_033557.2
• RefSeq Size: 3634
• RefSeq ORF: 2793
• Locus ID: 22591
• Gene Summary: Involved in global genome nucleotide excision repair (GG-NER) by acting as damage sensing and DNA-binding factor component of the XPC complex. Has only a low DNA repair activity by itself which is stimulated by Rad23b and Rad23a. Has a preference to bind DNA containing a short single-stranded segment but not to damaged oligonucleotides. This feature is proposed to be related to a dynamic sensor function: XPC can rapidly screen duplex DNA for non-hydrogen-bonded bases by forming a transient nucleoprotein intermediate complex which matures into a stable recognition complex through an intrinsic single-stranded DNA-binding activity. [UniProtKB/Swiss-Prot Function]