Vcl (NM_009502) Mouse Tagged ORF Clone

CAT#: MG211572

Vcl (GFP-tagged) - Mouse vinculin (Vcl)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Vcl
• Synonyms: 9430097D22; AA571387; AI462105; AW545629
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG211572 representing NM_009502
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGGTGTTTCACACGCGTACGATCGAGAGCATCCTGGAGCCGGTGGCGCAGCAGATCTCGCACCTGG
  TGATTATGCACGAGGAGGGCGAGGTGGACGGCAAAGCCATTCCTGACCTCACCGCGCCCGTAGCCGCCGT
  GCAGGCGGCCGTCAGCAACCTCGTCCGGGTTGGAAAAGAGACTGTTCAGACCACTGAGGATCAGATTCTG
  AAGAGAGATATGCCACCAGCCTTTATTAAGGTTGAAAATGCTTGCACCAAGCTTGTTCAGGCAGCCCAGA
  TGCTTCAGTCAGACCCATACTCGGTTCCTGCGCGGGATTACCTCATTGACGGCTCTAGGGGAATCCTTTC
  TGGCACATCTGACCTACTGCTTACCTTTGATGAGGCTGAGGTTCGTAAAATTATTAGGGTTTGCAAAGGA
  ATTTTGGAATATCTTACAGTGGCAGAGGTAGTGGAAACTATGGAAGACTTGGTCACTTACACAAAGAATC
  TTGGGCCAGGAATGACTAAGATGGCCAAAATGATTGATGAGAGACAGCAGGAGTTGACTCACCAGGAACA
  CCGTGTGATGTTGGTGAACTCAATGAACACTGTCAAAGAGCTGCTTCCTGTTCTCATTTCAGCTATGAAG
  ATTTTTGTTACAACCAAAAACTCAAAAAACCAAGGAATAGAAGAAGCTTTGAAAAATCGAAATTTTACTG
  TAGAAAAGATGAGTGCTGAAATTAACGAGATCATTCGTGTGTTACAACTCACTTCCTGGGATGAAGACGC
  CTGGGCCAGCAAGGACACTGAAGCCATGAAGAGAGCGCTGGCGTCCATAGACTCCAAATTGAACCAGGCC
  AAAGGTTGGCTCCGTGACCCCAATGCCTCCCCAGGGGATGCTGGAGAGCAGGCCATCAGGCAGATCTTAG
  ATGAAGCTGGAAAAGTTGGTGAACTTTGTGCAGGCAAGGAACGCAGGGAGATCCTAGGAACCTGCAAAAT
  GCTAGGGCAGATGACTGACCAAGTGGCTGACCTCCGAGCCAGAGGACAAGGAGCTTCCCCAGTGGCCATG
  CAGAAGGCCCAGCAAGTGTCTCAGGGGCTCGACGTGCTTACCGCCAAAGTGGAGAATGCAGCTCGGAAGC
  TGGAAGCCATGACGAACTCAAAGCAGAGCATTGCAAAGAAGATTGATGCTGCCCAGAATTGGCTGGCGGA
  TCCAAATGGTGGACCTGAGGGAGAAGAACAGATTCGAGGGGCTTTGGCTGAAGCTCGGAAGATTGCAGAA
  TTATGTGATGATCCTAAGGAGAGAGATGACATCCTCCGCTCCCTTGGAGAGATAGCTGCTCTGACCTCTA
  AACTAGGAGACTTGCGAAGACAGGGGAAAGGAGACTCGCCAGAGGCTCGAGCCTTGGCTAAACAAGTGGC
  GACGGCACTACAGAACCTGCAGACCAAAACCAACAGGGCCGTGGCCAACAGCAGACCTGCCAAAGCAGCT
  GTCCACCTCGAGGGCAAGATTGAACAGGCGCAGCGGTGGATTGATAACCCCACAGTAGATGACCGTGGAG
  TCGGTCAGGCTGCCATCCGTGGACTTGTGGCTGAGGGGCATCGGCTGGCCAATGTCATGATGGGACCTTA
  TCGCCAAGATCTTCTTGCCAAATGTGACCGTGTAGACCAGCTAACAGCTCAGCTGGCTGACCTGGCTGCC
  CGAGGGGAGGGGGAGAGTCCTCAGGCGAGAGCACTTGCATCCCAGCTTCAGGACTCCTTAAAGGATCTTA
  AAGCCCAGATGCAGGAAGCTATGACTCAAGAGGTATCCGATGTTTTCAGCGATACTACAACTCCTATCAA
  GCTGTTGGCAGTAGCCGCCACTGCTCCTCCTGATGCACCCAATAGGGAAGAGGTATTTGATGAAAGGGCA
  GCCAATTTTGAAAACCATTCAGGAAGGCTTGGAGCCACAGCAGAGAAGGCGGCTGCTGTTGGTACTGCCA
  ATAAATCGACAGTGGAAGGCATTCAGGCATCTGTGAAGACAGCCCGAGAACTCACTCCCCAGGTCATCTC
  CGCTGCTCGGATCTTACTGAGGAACCCTGGTAACCAGGCTGCTTATGAACATTTTGAGACCATGAAGAAC
  CAGTGGATTGATAATGTTGAAAAAATGACAGGGCTGGTGGACGAGGCTATTGATACCAAGTCTCTGTTGG
  ATGCTTCTGAAGAAGCAATTAAAAAAGACCTGGACAAGTGTAAGGTAGCCATGGCCAATATTCAGCCTCA
  GATGCTGGTCGCTGGAGCAACCAGTATTGCTCGTCGGGCCAACCGGATTCTGCTGGTTGCTAAGAGGGAG
  GTAGAGAACTCTGAGGACCCGAAGTTCCGAGAGGCTGTGAAAGCTGCCTCTGATGAACTGAGCAAAACAA
  TCTCCCCCATGGTGATGGATGCCAAGGCTGTGGCTGGAAACATCTCTGACCCTGGCCTGCAAAAGAGCTT
  CCTGGACTCAGGATATCGGATCCTGGGAGCTGTGGCCAAGGTCAGAGAAGCCTTCCAACCTCAGGAGCCT
  GACTTCCCGCCTCCTCCACCAGACCTTGAACAGCTACGACTAACTGATGAGCTGGCTCCTCCTAAGCCAC
  CTCTGCCTGAGGGTGAAGTCCCTCCACCCAGGCCCCCACCACCAGAAGAGAAGGATGAAGAGTTCCCTGA
  GCAGAAAGCTGGTGAGGTGATTAACCAGCCAATGATGATGGCCGCCAGGCAGCTCCACGATGAAGCTCGG
  AAATGGTCTAGCAAGGGCAATGACATCATTGCAGCAGCCAAGCGCATGGCTCTGCTGATGGCAGAGATGT
  CTCGGCTGGTAAGAGGGGGCAGTGGTACCAAGCGGGCACTTATTCAGTGTGCCAAGGATATCGCCAAGGC
  CTCTGATGAGGTGACGAGGTTGGCCAAGGAGGTTGCCAAGCAGTGCACAGATAAGCGGATTAGAACCAAT
  CTCTTACAGGTATGCGAGCGAATCCCAACTATAAGCACCCAGCTCAAAATCCTATCCACAGTGAAGGCCA
  CTATGCTGGGCCGGACCAACATCAGTGATGAGGAGTCTGAGCAGGCCACAGAGATGCTGGTTCATAATGC
  CCAGAACCTCATGCAGTCTGTGAAGGAGACTGTGCGAGAGGCTGAAGCTGCTTCAATCAAAATCCGAACA
  GATGCTGGCTTTACTCTGCGCTGGGTCAGAAAGACTCCCTGGTACCAG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG211572 representing NM_009502
  Red=Cloning site Green=Tags(s)
  
  MPVFHTRTIESILEPVAQQISHLVIMHEEGEVDGKAIPDLTAPVAAVQAAVSNLVRVGKETVQTTEDQIL
  KRDMPPAFIKVENACTKLVQAAQMLQSDPYSVPARDYLIDGSRGILSGTSDLLLTFDEAEVRKIIRVCKG
  ILEYLTVAEVVETMEDLVTYTKNLGPGMTKMAKMIDERQQELTHQEHRVMLVNSMNTVKELLPVLISAMK
  IFVTTKNSKNQGIEEALKNRNFTVEKMSAEINEIIRVLQLTSWDEDAWASKDTEAMKRALASIDSKLNQA
  KGWLRDPNASPGDAGEQAIRQILDEAGKVGELCAGKERREILGTCKMLGQMTDQVADLRARGQGASPVAM
  QKAQQVSQGLDVLTAKVENAARKLEAMTNSKQSIAKKIDAAQNWLADPNGGPEGEEQIRGALAEARKIAE
  LCDDPKERDDILRSLGEIAALTSKLGDLRRQGKGDSPEARALAKQVATALQNLQTKTNRAVANSRPAKAA
  VHLEGKIEQAQRWIDNPTVDDRGVGQAAIRGLVAEGHRLANVMMGPYRQDLLAKCDRVDQLTAQLADLAA
  RGEGESPQARALASQLQDSLKDLKAQMQEAMTQEVSDVFSDTTTPIKLLAVAATAPPDAPNREEVFDERA
  ANFENHSGRLGATAEKAAAVGTANKSTVEGIQASVKTARELTPQVISAARILLRNPGNQAAYEHFETMKN
  QWIDNVEKMTGLVDEAIDTKSLLDASEEAIKKDLDKCKVAMANIQPQMLVAGATSIARRANRILLVAKRE
  VENSEDPKFREAVKAASDELSKTISPMVMDAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAFQPQEP
  DFPPPPPDLEQLRLTDELAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEVINQPMMMAARQLHDEAR
  KWSSKGNDIIAAAKRMALLMAEMSRLVRGGSGTKRALIQCAKDIAKASDEVTRLAKEVAKQCTDKRIRTN
  LLQVCERIPTISTQLKILSTVKATMLGRTNISDEESEQATEMLVHNAQNLMQSVKETVREAEAASIKIRT
  DAGFTLRWVRKTPWYQ
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009502
• ORF Size: 3198 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_009502.4, NP_033528.3
• RefSeq Size: 5229
• RefSeq ORF: 3201
• Locus ID: 22330