Abi3bp (NM_001014399) Mouse Tagged ORF Clone

CAT#: MG215134

Abi3bp (GFP-tagged) - Mouse ABI gene family member 3 (NESH) binding protein (Abi3bp) transcript variant 3, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Abi3bp
• Synonyms: 5033411B22Rik; AI506287; BG172926; D930038M13Rik; eratin; TARSH
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG215134 representing NM_001014399
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTCTCCAGCCTGGGGTGTCTACTTCTCTGTGGAAGTATTGCACTTGCTCTGGGAAATGCACAGAAAT
  TGCCAAAAGGTAAAAAGCCAAGCTTGAAAGTGCACATCAATACCACCAGCGACTCCATCCTCTTGAAGTT
  CCTGCGTCCAAATGCAAACGTAAAGCTGGAAGGTTTTCTCCTGGGATATGGCAGCAATGTGTCTCCAAAC
  CAGTACTTCCCTCTTCCCACAGAAGGGAAATTCACAGAGGCTGTAGTTGATGCAGAGCCGAAGTATCTGA
  TAGTCGTACGACCTGCTCCTCCTCCCAGCCAGAAGAAGTCATGTTCAGGGAAATCTCGCCCTCGCAAACC
  CCTCCAGCTGGTGGTGGGTACGTTGACACCGAGTTCTGTCTTCCTGTCCTGGGGATTCCTCATCAACCCA
  CACCATGACTGGACATTGCCAAGCCACTGTCCCAGTGACAGATTTTATACAATTCGATATAGAGAAAAAG
  ATAAAGAAAAGAAATGGATTTTTCAACTCTGCCCAGCTACTGAAACAATTGTGGAAAATCTAAAGCCCAA
  CACAGTTTATGAATTTGGTGTGAAAGACAATATAGAAGGTGGAATTTGGAGTAAGATTTTCAATCATAAG
  ACTATTGTTGGAAGTAAAAACAAAGTAAACGGGAAAATCCAAAGCACTTATGACCAGGTCCACTCAGTGC
  CAGTCCCGAGGAAGCTAATCCCACTAACGATCATTAAGCAAGTGATTCAGAATGTTACTCACAGGGCTTC
  AACCAAATCCCCAGACAAGACTCCTTTTGGGGGAACAATATTAGTTCATCTGATTATTCCGGGTCTTAAT
  GAATCCACTGTAAAACTTCCCACATCCATAATGCTTGAGATTTCCGATGCTCTCAAGGCACAATTAGCTA
  AGAATGAAACATTGGCTTTACCAGCAGAATCTAAAACACCAGAGGTCGAAAAATTGGCAGGGCAGCCAGT
  AACAGTGACTCCGGAATCAGTTTCAAGAAGCACTAAGCCCACCCTGTCTAGTGCTTTAGACACTGCAGAG
  ACAGCACTGGCACCCAGGCAGACAACCAGCATGCCTCCCAAGCTCAAAACACCACACTCAAGGATGCCAG
  CAAAAGAGCCAGTTCCTAAGGAACCCCTGCATACGACTTCAAAGCCAAAAATGCCACCAAGTCCTGAAGT
  GGCAGACACTACATCTGCACCTTTAGAGACACGGGGCATTCCTCTCATACCTGTGATTTCACCAAGACCT
  AGTCAAGAGGAACTACAAACCGCCATGGAAGAAACAGACCAGTCTACCCAAGAGCTCTTCACGACTAAGA
  TTCCACGAACAACTGAATTGGCAAAGACAACTCAGGCACCACACAGATTGCATACAGCTCCTGTGAGGCC
  CAGAATACCTGGCAGGCCACATGGCAGGCCTGCTCTGAACAAAACAACTACAAGGCCTGATAAAACCAAA
  CCCAGAGGGACGTCCCATAAAAACGGAGTAGGAACAGGAACCAAGCAAGCACCTAAGCCACCAAGTCCTG
  GGAGAAATGCGTCAGTGGATTCACATGCCACAAGAAAACCAGGCTCAGTCTCAGGGACGCGTCGTCCACC
  CATACCACATAGGCATTCGTCCACTAGACCTGTGTCTCCAGAGAGAAGGCCTTTACCCCCGAATAATGTC
  ACCGGGAAGCCAGGACGTGCGGGAATTGTTTCCTCCAGCCGGGTAACGTCCCCACCCCTGAAGGCAACAC
  TCCACCCTATCGGAACAGCCACAGCGAGACCAGGGGCAGAACAAAAGGAACCAACTGCTCCTGCTTCAGA
  AGAAGAGTTTGGTACTACAACTGACTTCAGTTCAAGTCCCACCAAAGAAACTGATCCCCTTGGGAAGCCC
  AGGTTCATAGGTCCCCATGTGCGATATATCCCAAAGCCTGAAAACAAGCCCTGTTCCATCACTGACTCAG
  TCAGACGGTTCCCTACAGAGGAAGCCACGGAGGGGAATGCCACCAGCCCACCACAGAACCCACCCACCAA
  CCTCACTGTGGTCACTGTCGAAGGCTGCCCCTCATTTGTCATCTTGGACTGGGAAAAACCTCTAAATGAC
  ACTGTCACTGAATATGAAGTCATATCCAGAGAAAATGGGTCATTCAGTGGGAAGAACAAGTCCATTCAAA
  TTACCAATCAAACGTTCTCTACAGTAGAAAACCTGAAACCAGATACAAGCTATGAATTCCAGGTGAAACC
  CAAAAATCCACTTGGTGAGGGCCCCGCCAGCAACACGGTAGCGTTCAGTACTGAATCAGCTGACCCAAGA
  GTGAGTGAGCCAATTTCTGCAGGAAGAGATGCCATCTGGACTGAAAGACCTTTTAACTCAGACTCTTACT
  CAGAATGCAAAGGCAAACAGTATGTTAAAAGGACGTGGTATAAAAAATTTGTCGGAGTGCAGCTATGTAA
  CTCTCTCAGATACAAGATTTATTTGAGCGATTCCCTCACAGGCAAGTTTTACAACATAGGAGATCAGAGG
  GGCCACGGAGAAGATCACTGCCAGTTTGTGGATTCATTTTTAGATGGTCGCACAGGACAGCAACTCACCT
  CTGAACAGTTACCCACCAAAGAAGGCTATTTCAGAGCTGTTCGTCAGGAACCTGTCCAATTTGGAGAAAT
  AGGTGGTCATACCCAAATCAATTATGTCCAGTGGTATGAATGTGGGACCACAATACCTGGAAAATGG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG215134 representing NM_001014399
  Red=Cloning site Green=Tags(s)
  
  MLSSLGCLLLCGSIALALGNAQKLPKGKKPSLKVHINTTSDSILLKFLRPNANVKLEGFLLGYGSNVSPN
  QYFPLPTEGKFTEAVVDAEPKYLIVVRPAPPPSQKKSCSGKSRPRKPLQLVVGTLTPSSVFLSWGFLINP
  HHDWTLPSHCPSDRFYTIRYREKDKEKKWIFQLCPATETIVENLKPNTVYEFGVKDNIEGGIWSKIFNHK
  TIVGSKNKVNGKIQSTYDQVHSVPVPRKLIPLTIIKQVIQNVTHRASTKSPDKTPFGGTILVHLIIPGLN
  ESTVKLPTSIMLEISDALKAQLAKNETLALPAESKTPEVEKLAGQPVTVTPESVSRSTKPTLSSALDTAE
  TALAPRQTTSMPPKLKTPHSRMPAKEPVPKEPLHTTSKPKMPPSPEVADTTSAPLETRGIPLIPVISPRP
  SQEELQTAMEETDQSTQELFTTKIPRTTELAKTTQAPHRLHTAPVRPRIPGRPHGRPALNKTTTRPDKTK
  PRGTSHKNGVGTGTKQAPKPPSPGRNASVDSHATRKPGSVSGTRRPPIPHRHSSTRPVSPERRPLPPNNV
  TGKPGRAGIVSSSRVTSPPLKATLHPIGTATARPGAEQKEPTAPASEEEFGTTTDFSSSPTKETDPLGKP
  RFIGPHVRYIPKPENKPCSITDSVRRFPTEEATEGNATSPPQNPPTNLTVVTVEGCPSFVILDWEKPLND
  TVTEYEVISRENGSFSGKNKSIQITNQTFSTVENLKPDTSYEFQVKPKNPLGEGPASNTVAFSTESADPR
  VSEPISAGRDAIWTERPFNSDSYSECKGKQYVKRTWYKKFVGVQLCNSLRYKIYLSDSLTGKFYNIGDQR
  GHGEDHCQFVDSFLDGRTGQQLTSEQLPTKEGYFRAVRQEPVQFGEIGGHTQINYVQWYECGTTIPGKW
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001014399
• ORF Size: 2727 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001014399.2, NP_001014421.2
• RefSeq Size: 3884 bp
• RefSeq ORF: 2730 bp
• Locus ID: 320712
• Cytogenetics: 16 C1.1