Dusp13 (NM_001007268) Mouse Tagged ORF Clone

CAT#: MG219056

Dusp13 (GFP-tagged) - Mouse dual specificity phosphatase 13 (Dusp13) transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Dusp13
• Synonyms: DUSP13A; DUSP13B; Gm1203; LMW-DSP6; MDSP; TMDP; TS-DSP6
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG219056 representing NM_001007268
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGATGCCTCCATCCCAAAACCTGGGGAAGAAAAAGAAGCCACACCTTGCCCCAGTATCCTGCAGC
  TAGAGGAGCTCCTGAGGGCCGGGAGAGCCTCTTGTAGTAGAGTGGATGAAGTCTGGCCCAACCTTTTCAT
  CGGAGATGCGGCCACGGCAAATAACCGATTTGAGCTGTGGAAGTTGGGGATCACCCATGTGCTGAATGCC
  GCCCACGGAGGACTCTACTGTCAGGGGGGTCCTGACTTCTACGGCAGCAGTGTGTGCTACCTGGGGATCC
  CAGCCCACGACCTCCCTGATTTCAATATCAGCCCCTACTTCTCCTCAGCAGCTGACTTCATCCACCGAGC
  CCTCACCGTACCTGGAGCTAAGGTGCTGGTGCACTGCGTGGTGGGTGTGAGCCGATCTGCCACACTGGTC
  CTGGCTTACCTCATGCTCCACCAGCAGCTGTCCTTGCAGCAGGCCATAATCACTGTGAGGGAGCGCCGAT
  GGATCTTCCCCAATCGTGGCTTCCTCCGACAGCTCTGCCAGCTGGACCAGCAACTTCGGGGTGCAGGTCA
  GAGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG219056 representing NM_001007268
  Red=Cloning site Green=Tags(s)
  
  MADASIPKPGEEKEATPCPSILQLEELLRAGRASCSRVDEVWPNLFIGDAATANNRFELWKLGITHVLNA
  AHGGLYCQGGPDFYGSSVCYLGIPAHDLPDFNISPYFSSAADFIHRALTVPGAKVLVHCVVGVSRSATLV
  LAYLMLHQQLSLQQAIITVRERRWIFPNRGFLRQLCQLDQQLRGAGQS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001007268
• ORF Size: 564 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001007268.1, NP_001007269.1
• RefSeq Size: 1745 bp
• RefSeq ORF: 567 bp
• Locus ID: 27389
• Cytogenetics: 14 A3
• Gene Summary: Members of the protein-tyrosine phosphatase superfamily cooperate with protein kinases to regulate cell proliferation and differentiation. This superfamily is separated into two families based on the substrate that is dephosphorylated. One family, the dual specificity phosphatases (DSPs) acts on both phosphotyrosine and phosphoserine/threonine residues. This gene encodes different but related DSP proteins through the use of non-overlapping open reading frames, alternate splicing, and presumed different transcription promoters. Expression of the distinct proteins from this gene has been found to be tissue specific and the proteins may be involved in postnatal development of specific tissues. A protein encoded by the upstream ORF was found in skeletal muscle, whereas the encoded protein from the downstream ORF was found only in testis. In humans, a similar pattern of expression was found. Multiple alternatively spliced transcript variants were described, but the full-length sequence of only some were determined. [provided by RefSeq, Jul 2008]