Clcn1 (NM_013491) Mouse Tagged ORF Clone

CAT#: MG220414

Clcn1 (GFP-tagged) - Mouse chloride channel 1 (Clcn1), (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Clcn1
• Synonyms: adr; Clc-1; Clc1; mto; myotonia; nmf355; SMCC1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG220414 representing NM_013491
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCGGTCCCAGTCCCAGCGGCATGGAGGGGAACAGAGCTGGTGGGGCAGTGCCCCCCAGTACCAGT
  ACATGCCCTTTGAACATTGTACCAGCTACGGACTGCCCTCAGAGAATGGGGGCCTTCAGCACCGGCCCCG
  AAAGGACATGGGTCCCAGGCACAATGCCCACCCAACACAGATATATGGCCATCAAAAAGAACAATATTCA
  TATAAGGCACAGGACGGGGGAATGCCCAAGAAGATGGGCTCCAGTTCTACCATGGACAGCTTGGATGAGG
  ACCACTATTCTAAATGTCAAGACTGTGTCCATCGCCTGGGACGTGTGCTGAGAAGGAAGCTGGGGGAAGA
  TTGGATCTTTCTTGTGCTCCTGGGCCTCCTGATGGCTCTAGTCAGCTGGTGCATGGACTATGTTAGTGCC
  AAGAGCCTTCAGGCCTACAAGTGGACCTATGCCCAGATGAAGCCTAGCCTTCCTCTGCAGTACCTGGCCT
  GGGTCACCTTCCCACTTATCCTGATCCTCTTCAGCGCCCTCTTTTGCCAACTCATCTCTCCCCAGGCTGT
  GGGCTCTGGAATCCCTGAGATGAAAACAATTCTTCGTGGTGTTGTCTTGAAGGAATACCTCACACTCAAG
  GCCTTTGTAGCCAAGGTGGTAGCTTTGACAGCTGGACTGGGCAGTGGCATCCCTGTGGGGAAAGAGGGTC
  CCTTTGTTCACATCGCCAGCATCTGTGCTGCTGTCCTCAGCAAGTTTATGTCCATGTTCTCTGGTGTCTA
  TGAGCAGCCATACTATTACACTGACATCCTGACAGTGGGCTGTGCCGTGGGGGTCGGTTGCTGTTTTGGA
  ACACCACTTGGAGGAGTGCTATTTAGCATCGAGGTCACCTCTACCTACTTTGCTGTTCGGAATTACTGGC
  GAGGATTCTTTGCAGCCACATTCAGTGCCTTTGTGTTCCGTGTCCTGGCCGTTTGGAACAAGGATGCTGT
  CACCATCACTGCTCTGTTCAGAACGAATTTCCGAATGGATTTCCCCTTTGACCTGAAGGAACTCCCAGCT
  TTTGCTGTCATTGGGATTTGCTGCGGGTTCTTGGGAGCTGTTTTCGTTTATCTGCATCGCCAAGTCATGC
  TCGGTGTCCGAAAGCACAAGTGTCTCAGCCAATTTCTTGCTAAGCACCGCCTGCTATATCCTGGAATTGT
  TACCTTTGTCATCGCCTCGCTCACATTTCCACCAGGAATGGGTCAATTCATGGCTGGAGAGCTGATGCCC
  CGTGAAGCTATCAGTACCCTCTTTGACAACAACACATGGGTAAAGCACATAGGTGACCCCCAAAGCTTGG
  GCCAGTCAGCTGTGTGGCTTCACCCCCAGGTCAACGTGATCATCATCATCCTTCTCTTCTTCGTTATGAA
  GTTTTGGATGTCCATTGTAGCCACCACTATGCCCATACCCTGTGGAGGCTTCATGCCTGTTTTTGTGCTA
  GGAGCTGCATTTGGAAGGCTGGTAGGAGAGATCATGGCCATGCTATTCCCTGAGGGTATCTTATTTGATG
  ATATCATCTATAAGATCTTACCTGGGGGCTATGCAGTAATTGGAGCAGCAGCTTTGACAGGGGCTGTCTC
  CCACACAGTCTCCACAGCCGTCATTTGCTTCGAATTAACCGGTCAGATTGCTCACATCCTGCCCATGATG
  GTGGCTGTTATCTTGGCCAACATGGTGGCTCAGAGTCTGCAGCCCTCCCTCTATGACAGCATCATCCAGG
  TCAAGAAGCTTCCCTATCTGCCAGACCTTGGTTGGAACCAGCTCAGCAAATTTACAATTTTTGTTGAGGA
  CATCATGGTACGTGATGTGAAGTTCGTTTCAGCTTCTTGTACATATGGGGAACTGAGAAACCTACTCCAG
  GCCACCACAGTCAAGACTTTACCATTGGTTGACTCCAAAGATTCAATGATCCTGCTGGGCTCTGTGGAAC
  GCTCAGAACTGCAGTCCCTCCTGCAGCGCCACCTGTGTGCAGAGCGAAGGTTGAAGGCTGCCCAGGACAT
  GGCTCGAAAGTTATCAGAGCTGCCTTATAATGGCAAGGCTCAGCTGGCTGGGGACTGGCATCCTGGTGGT
  CGACCTGAGTCCTTTGCCTTCGTAGATGAAGATGAAGATGAGGACCTCTCCAGGAAGATGGAGCTTCCAC
  TGACTCCAGCTCCTCCCCCTCCTTCCCCTCCTCCTCCACCTTCCCAGTTTCCTATTGCTCCATCAAACCC
  TGAAGAACCTAATGGGCCACTGCCCAGCCATAAACAGCCACCTGAAGCCTCAGACTCTGCAGATCAAAGA
  TCATCCACCTTCCAACGCCTGCTGCACTGCTTGCTAGGAAAAGCTCACTCCAAAAAGAAGAAAATAACAC
  AGGATTCAACAGATTTAGTGGATAACATGTCACCTGAAGAGATTGAGGCCTGGGAGCGGGAGCAGCTGAG
  CCAGCCTGTGTGCTTTGATTGCTGCTGCATCGACCAGTCTCCCTTCCAGCTGGTGGAGCAGACAACCCTG
  CACAAGACTCATACACTCTTCTCACTTCTTGGCCTCCATCTTGCCTATGTGACCAGCATGGGGAAGCTCA
  GAGGTGTCTTGGCACTAGAAGAGCTACAGAAAGCTATTGAGGGCCACACCAAATCTGGGGTGCAGCTTCG
  CCCTCCACTTGCCAGCTTCCGGAATACAACTTCAATCCGGAAGACTCCTGGGGGGCCACCCCCTCCTGCA
  GAGGGCTGGAATGTACCTGAGGATGGAGATGGGGCTCCTGGAAGAGAAGTCATGGTTCCTACCATGCCAG
  AGACTCCTGTCCCACCACCATCTCCAGAGGCCCCTTCCTGCCTGGCCCCAGCCAGAGCGGAGGGTGAGCT
  GGAGGAACTGGAGATGGTGGGGAGCCTAGAGCCTGAGGAGGAGCTGGCTGACATCTTGCATGGCCCCAGT
  CTGCGGTCCACTGATGAGGAAGATGAGGACGAGCTGATCCTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG220414 representing NM_013491
  Red=Cloning site Green=Tags(s)
  
  MERSQSQRHGGEQSWWGSAPQYQYMPFEHCTSYGLPSENGGLQHRPRKDMGPRHNAHPTQIYGHQKEQYS
  YKAQDGGMPKKMGSSSTMDSLDEDHYSKCQDCVHRLGRVLRRKLGEDWIFLVLLGLLMALVSWCMDYVSA
  KSLQAYKWTYAQMKPSLPLQYLAWVTFPLILILFSALFCQLISPQAVGSGIPEMKTILRGVVLKEYLTLK
  AFVAKVVALTAGLGSGIPVGKEGPFVHIASICAAVLSKFMSMFSGVYEQPYYYTDILTVGCAVGVGCCFG
  TPLGGVLFSIEVTSTYFAVRNYWRGFFAATFSAFVFRVLAVWNKDAVTITALFRTNFRMDFPFDLKELPA
  FAVIGICCGFLGAVFVYLHRQVMLGVRKHKCLSQFLAKHRLLYPGIVTFVIASLTFPPGMGQFMAGELMP
  REAISTLFDNNTWVKHIGDPQSLGQSAVWLHPQVNVIIIILLFFVMKFWMSIVATTMPIPCGGFMPVFVL
  GAAFGRLVGEIMAMLFPEGILFDDIIYKILPGGYAVIGAAALTGAVSHTVSTAVICFELTGQIAHILPMM
  VAVILANMVAQSLQPSLYDSIIQVKKLPYLPDLGWNQLSKFTIFVEDIMVRDVKFVSASCTYGELRNLLQ
  ATTVKTLPLVDSKDSMILLGSVERSELQSLLQRHLCAERRLKAAQDMARKLSELPYNGKAQLAGDWHPGG
  RPESFAFVDEDEDEDLSRKMELPLTPAPPPPSPPPPPSQFPIAPSNPEEPNGPLPSHKQPPEASDSADQR
  SSTFQRLLHCLLGKAHSKKKKITQDSTDLVDNMSPEEIEAWEREQLSQPVCFDCCCIDQSPFQLVEQTTL
  HKTHTLFSLLGLHLAYVTSMGKLRGVLALEELQKAIEGHTKSGVQLRPPLASFRNTTSIRKTPGGPPPPA
  EGWNVPEDGDGAPGREVMVPTMPETPVPPPSPEAPSCLAPARAEGELEELEMVGSLEPEEELADILHGPS
  LRSTDEEDEDELIL
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013491
• ORF Size: 2982 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_013491.2, NP_038519.1
• RefSeq Size: 3545
• RefSeq ORF: 2985
• Locus ID: 12723