Piwil1 (NM_021311) Mouse Tagged ORF Clone

CAT#: MG222848

Piwil1 (GFP-tagged) - Mouse piwi-like homolog 1 (Drosophila) (Piwil1), (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Piwil1
• Synonyms: MIWI
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG222848 representing NM_021311
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTGGCCGAGCCCGAGCTCGGGCCCGCGGCAGGGCACGAGGTCAGGAGACGGTGCAGCATGTTGGGG
  CTGCTGCGAGCCAGCAACCTGGGTACATCCCACCGAGACCTCAACAGTCCCCCACAGAGGGGGACTTGGT
  TGGCCGAGGACGACAGAGGGGGATGGTAGTCGGAGCCACATCCAAGTCACAAGAACTGCAGATCTCAGCT
  GGGTTTCAGGAGCTGTCACTGGCAGAGAGAGGAGGGCGTCGCCGAGACTTCCATGACCTTGGTGTGAACA
  CCAGACAGAACCTTGACCATGTCAAAGAGTCAAAGACAGGCTCCTCTGGCATCATTGTGAAGCTGAGCAC
  CAACCACTTCCGGCTGACCTCGCGCCCACAGTGGGCCCTGTATCAGTACCACATCGACTACAATCCCCTG
  ATGGAGGCCCGAAGGCTTCGCTCCGCACTGCTCTTCCAGCATGAAGACCTCATTGGAAGGTGTCATGCTT
  TCGATGGGACAATATTGTTTTTACCTAAGAGACTACAGCACAAGGTCACAGAAGTATTCAGTCAGACTCG
  GAATGGGGAACACGTGAGGATCACCATCACCCTGACCAACGAGCTGCCGCCCACCTCGCCCACCTGCCTG
  CAGTTCTATAACATCATCTTCAGGAGGCTCTTGAAAATCATGAATTTGCAACAAATTGGACGGAATTATT
  ACAATCCAAGTGACCCGATTGATATTCCAAACCACAGGTTGGTGATCTGGCCCGGCTTCACCACCTCCAT
  CCTTCAGTATGAGAACAACATCATGCTCTGCACAGACGTCAGCCACAAGGTGCTCCGCAGCGAGACTGTC
  CTAGACTTCATGTTCAATCTATACCAGCAGACAGAGGAGCACAAGTTCCAGGAGCAAGTGTCGAAGGAGC
  TCATAGGCCTCATCGTTCTCACCAAGTACAATAACAAGACCTACCGGGTGGATGACATTGACTGGGACCA
  GAATCCAAAGAGCACCTTCAAGAAGGCGGATGGCTCGGAGGTCAGCTTCCTGGAGTACTACAGGAAGCAA
  TACAACCAGGAGATCACGGACCTGAAGCAGCCGGTGCTGGTGAGCCAACCCAAGCGGAGGAGAGGCCCCG
  GCGGCACCCTGCCTGGCCCAGCTATGCTCATCCCTGAACTCTGCTATCTCACAGGCCTGACTGATAAAAT
  GCGCAATGATTTCAATGTGATGAAGGACCTGGCAGTGCACACGCGGCTGACCCCTGAGCAGCGGCAGCGG
  GAGGTGGGCCGCCTCATCGACTACATCCACAAGGATGACAATGTGCAGAGAGAGCTTCGAGACTGGGGCC
  TGAGCTTCGACTCAAACTTGCTGTCCTTCTCTGGAAGAATCTTACAATCTGAGAAGATCCACCAGGGCGG
  AAAGACGTTTGATTACAACCCACAATTTGCAGACTGGTCCAAAGAAACAAGAGGCGCGCCGCTGATCAGC
  GTGAAGCCATTGGATAACTGGCTGCTGATCTATACCCGCAGGAATTATGAAGCAGCCAACTCACTGATAC
  AGAACCTGTTCAAAGTGACTCCAGCCATGGGCATCCAGATGAAAAAGGCAATCATGATCGAGGTGGATGA
  CAGAACAGAAGCTTATCTGAGAGCCTTGCAGCAGAAGGTGACGTCAGACACTCAGATAGTTGTCTGTCTC
  TTGTCAAGTAATCGGAAGGACAAATATGATGCCATCAAGAAGTACTTGTGTACAGACTGCCCCACCCCAA
  GTCAGTGTGTGGTGGCCCGGACCCTGGGCAAGCAGCAAACAGTCATGGCCATTGCCACCAAGATCGCCCT
  GCAGATGAACTGCAAGATGGGAGGCGAGCTCTGGCGGGTGGACATGCCCCTGAAACTGGCAATGATCGTG
  GGCATCGACTGTTACCATGACACCACAGCTGGGCGGAGGTCCATCGCAGGATTCGTCGCCAGCATCAATG
  AAGGGATGACCCGCTGGTTCTCCCGCTGCGTCTTTCAGGACCGCGGGCAGGAGCTGGTGGATGGTCTCAA
  GGTGTGCTTGCAAGCTGCTCTGAGGGCTTGGAGTGGCTGCAATGAATACATGCCCAGCCGTGTCATCGTG
  TACCGAGACGGTGTGGGGGACGGGCAGCTGAAGACCCTGGTCAATTATGAGGTCCCACAGTTCCTAGATT
  GCCTCAAGTCAGTCGGGAGAGGTTACAACCCAAGACTGACTGTAATCGTGGTGAAGAAGCGTGTCAATGC
  CAGGTTTTTTGCTCAGTCTGGGGGAAGACTTCAGAACCCTCTTCCAGGGACAGTCATCGATGTGGAAGTC
  ACCAGACCAGAGTGGTATGACTTTTTCATCGTGAGCCAGGCAGTGAGAAGCGGGAGTGTGTCCCCAACAC
  ACTACAATGTCATCTATGACAGCAGTGGCCTGAAGCCCGACCACATCCAGCGGCTGACATACAAGCTCTG
  CCACGTGTACTATAATTGGCCTGGAGTCATCCGAGTCCCTGCACCTTGCCAGTATGCACACAAGCTGGCC
  TTCCTCGTGGGCCAGAGCATCCACAGAGAGCCAAACCTCTCCCTGTCCAACCGCCTCTACTACCTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG222848 representing NM_021311
  Red=Cloning site Green=Tags(s)
  
  MTGRARARARGRARGQETVQHVGAAASQQPGYIPPRPQQSPTEGDLVGRGRQRGMVVGATSKSQELQISA
  GFQELSLAERGGRRRDFHDLGVNTRQNLDHVKESKTGSSGIIVKLSTNHFRLTSRPQWALYQYHIDYNPL
  MEARRLRSALLFQHEDLIGRCHAFDGTILFLPKRLQHKVTEVFSQTRNGEHVRITITLTNELPPTSPTCL
  QFYNIIFRRLLKIMNLQQIGRNYYNPSDPIDIPNHRLVIWPGFTTSILQYENNIMLCTDVSHKVLRSETV
  LDFMFNLYQQTEEHKFQEQVSKELIGLIVLTKYNNKTYRVDDIDWDQNPKSTFKKADGSEVSFLEYYRKQ
  YNQEITDLKQPVLVSQPKRRRGPGGTLPGPAMLIPELCYLTGLTDKMRNDFNVMKDLAVHTRLTPEQRQR
  EVGRLIDYIHKDDNVQRELRDWGLSFDSNLLSFSGRILQSEKIHQGGKTFDYNPQFADWSKETRGAPLIS
  VKPLDNWLLIYTRRNYEAANSLIQNLFKVTPAMGIQMKKAIMIEVDDRTEAYLRALQQKVTSDTQIVVCL
  LSSNRKDKYDAIKKYLCTDCPTPSQCVVARTLGKQQTVMAIATKIALQMNCKMGGELWRVDMPLKLAMIV
  GIDCYHDTTAGRRSIAGFVASINEGMTRWFSRCVFQDRGQELVDGLKVCLQAALRAWSGCNEYMPSRVIV
  YRDGVGDGQLKTLVNYEVPQFLDCLKSVGRGYNPRLTVIVVKKRVNARFFAQSGGRLQNPLPGTVIDVEV
  TRPEWYDFFIVSQAVRSGSVSPTHYNVIYDSSGLKPDHIQRLTYKLCHVYYNWPGVIRVPAPCQYAHKLA
  FLVGQSIHREPNLSLSNRLYYL
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021311
• ORF Size: 2586 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_021311.3, NP_067286.1
• RefSeq Size: 3944
• RefSeq ORF: 2589
• Locus ID: 57749