Mbd3 (NM_013595) Mouse Tagged ORF Clone

CAT#: MG222862

Mbd3 (GFP-tagged) - Mouse methyl-CpG binding domain protein 3 (Mbd3), (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Mbd3
• Synonyms: AI181826; AU019209
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG222862 representing NM_013595
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCGGAAGAGGTGGGAGTGCCCGGCGCTCCCGCAGGGCTGGGAAAGGGAAGAAGTGCCCAGGAGGT
  CGGGGCTGTCGGCCGGCCACAGGGATGTCTTTTACTATAGCCCCAGCGGGAAGAAGTTCCGCAGCAAGCC
  ACAACTGGCACGTTACCTGGGCGGATCCATGGACCTCAGCACCTTCGACTTCCGCACCGGAAAGATGTTG
  ATGAACAAGATGAATAAGAGTCGCCAGCGTGTGCGCTATGATTCTTCCAACCAGGTCAAGGGCAAGCCTG
  ACCTGAACACCGCGCTGCCTGTACGGCAGACTGCATCCATCTTCAAGCAACCGGTGACCAAGATCACCAA
  CCACCCCAGCAACAAGGTCAAGAGCGACCCGCAGAAGGCAGTGGACCAGCCGAGGCAGCTTTTCTGGGAG
  AAGAAGCTAAGTGGATTGAGTGCCTTTGACATTGCAGAAGAACTGGTCAGGACCATGGACTTGCCCAAGG
  GCCTGCAGGGAGTGGGCCCTGGCTGTACAGATGAGACGCTGCTGTCAGCCATTGCGAGTGCTCTACACAC
  CAGCACCCTGCCCATTACAGGCCAGCTCTCTGCAGCCGTGGAGAAGAACCCTGGTGTGTGGCTGAACACT
  GCACAGCCACTGTGCAAAGCCTTCATGGTGACAGATGACGACATCAGGAAGCAGGAGGAGCTGGTACAGC
  AGGTACGGAAGCGCCTGGAGGAGGCACTGATGGCCGACATGCTAGCTCATGTGGAGGAGCTTGCCCGAGA
  CGGGGAGGCACCACTGGACAAGGCCTGTGCAGAGGAGGAAGAGGAGGAGGAAGAGGAGGAGGAAGAGCCG
  GAGCCAGAGCGAGTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG222862 representing NM_013595
  Red=Cloning site Green=Tags(s)
  
  MERKRWECPALPQGWEREEVPRRSGLSAGHRDVFYYSPSGKKFRSKPQLARYLGGSMDLSTFDFRTGKML
  MNKMNKSRQRVRYDSSNQVKGKPDLNTALPVRQTASIFKQPVTKITNHPSNKVKSDPQKAVDQPRQLFWE
  KKLSGLSAFDIAEELVRTMDLPKGLQGVGPGCTDETLLSAIASALHTSTLPITGQLSAAVEKNPGVWLNT
  AQPLCKAFMVTDDDIRKQEELVQQVRKRLEEALMADMLAHVEELARDGEAPLDKACAEEEEEEEEEEEEP
  EPERV
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013595
• ORF Size: 855 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_013595.2, NP_038623.1
• RefSeq Size: 1589
• RefSeq ORF: 858
• Locus ID: 17192
• Gene Summary: This gene encodes a member of the MBD family of nuclear proteins that contain a methyl-CpG binding domain (MBD). The encoded protein is a component of the nucleosome remodeling and histone deacetylation (NuRD) complex. Deletion of this gene causes embryonic lethality in mice. Embryonic stem cells lacking the encoded protein are severely compromised in their ability to differentiate and fail to commit to developmental lineages in the absence of leukemia inhibitory factor. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2015]