Nfil3 (NM_017373) Mouse Tagged ORF Clone

CAT#: MG223366

Nfil3 (GFP-tagged) - Mouse nuclear factor interleukin 3 regulated (Nfil3), (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Nfil3
• Synonyms: AV225605; E4BP4
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG223366 representing NM_017373
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGGCGCGCCC
  
  ATGCAGCTGAGAAAAATGCAGACCATCAAAAAGGAGCCCGCACCCCTAGATCCTACCAGCAGCTCAGACA
  AGATGCTGCTGCTGAACTCTGCCTTAGCTGAGGTGGCCGAGGACCTAGCCTCAGGTGAAGATTTGCTCCT
  GAACGAAGGGAGCATGGGGAAAAACAAATCCTCGGCGTGTCGGAGAAAACGGGAATTCATTCCGGACGAG
  AAGAAAGACGCCATGTATTGGGAGAAACGGCGGAAAAACAACGAAGCTGCCAAAAGATCTCGGGAGAAGC
  GCCGCCTCAATGACCTGGTTTTGGAGAACAAGCTGATTGCCCTGGGAGAAGAAAATGCCACTTTAAAAGC
  TGAGCTGCTCTCCCTGAAATTAAAGTTTGGTTTAATTAGCTCCACGGCGTATGCCCAAGAAATCCAGAAA
  CTCAGTAATTCCACAGCTGTCTACTTTCAGGACTACCAGACATCCAAGGCTGCCGTGAGCTCTTTTGTGG
  ACGAGCATGAGCCTGCGATGGTAGCCGGAAGTTGCATCTCAGTCATCAAGCACTCTCCCCAGAGCTCGCT
  CTCCGATGTGTCAGAGGTGTCCTCGGTGGAGCACACTCAGGAAAGCCCCGCACAGGGAGGCTGCCGGAGC
  CCTGAGAACAAGTTCCCTGTGATCAAGCAGGAGCCCGTGGAGTTGGAGAGCTTTGCCAGGGAGGCCAGGG
  AGGAGCGGGGCACGTATTCCACCTCCATCTACCAGAGCTACATGGGAAGCTCTTTCTCCACTTACTCCCA
  CTCCCCACCCCTCTTGCAGGTCCATGGGTCCACTAGCAACTCCCCAAGAACCTCAGAGGCCGATGAGGGT
  GTAGTGGGCAAGTCTTCTGATGGGGAAGACGAACAACAGGTCCCTAAGGGCCCCATCCATTCTCCAGTGG
  AGCTGCAACGGGTTCACGCCACGGTGGTGAAGGTTCCGGAAGTGAACCCTTCTGCCTTACCGCACAAGCT
  TCGGATTAAAGCCAAGGCCATGCAGGTCAAAGTGGAGGCTTTGGACAGCGAGTTTGAAGGCATGCAGAAA
  CTCTCTTCACCCGCCGATGCGATCGCCAAAAGACATTTTGACCTGGAGAAACATGGAACCTCGGGTATGG
  CCCATTCCTCCCTCCCTCCTTTCTCAGTGCAGGTGACGAACATTCAAGATTGGTCCCTCAAATCGGAACA
  CTGGCATCACAAAGAACTGAGCAGCAAAACTCAGAGTAGCTTCAAAACAGGTGTGGTGGAAGTCAAAGAC
  GGTGGCTATAAGGTTTCCGAAGCTGAGAATTTGTATTTGAAGCAGGGAATAGCAAACTTATCTGCAGAGG
  TGGTCTCGCTCAAGAGATTCATAGCCACACAACCGATCTCGGCTTCGGACTCCAGG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG223366 representing NM_017373
  Red=Cloning site Green=Tags(s)
  
  MQLRKMQTIKKEPAPLDPTSSSDKMLLLNSALAEVAEDLASGEDLLLNEGSMGKNKSSACRRKREFIPDE
  KKDAMYWEKRRKNNEAAKRSREKRRLNDLVLENKLIALGEENATLKAELLSLKLKFGLISSTAYAQEIQK
  LSNSTAVYFQDYQTSKAAVSSFVDEHEPAMVAGSCISVIKHSPQSSLSDVSEVSSVEHTQESPAQGGCRS
  PENKFPVIKQEPVELESFAREAREERGTYSTSIYQSYMGSSFSTYSHSPPLLQVHGSTSNSPRTSEADEG
  VVGKSSDGEDEQQVPKGPIHSPVELQRVHATVVKVPEVNPSALPHKLRIKAKAMQVKVEALDSEFEGMQK
  LSSPADAIAKRHFDLEKHGTSGMAHSSLPPFSVQVTNIQDWSLKSEHWHHKELSSKTQSSFKTGVVEVKD
  GGYKVSEAENLYLKQGIANLSAEVVSLKRFIATQPISASDSR
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: AscI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017373
• ORF Size: 1386 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_017373.3, NP_059069.1
• RefSeq Size: 2019
• RefSeq ORF: 1389
• Locus ID: 18030
• Gene Summary: The protein encoded by this gene is a transcriptional regulator that binds as a homodimer to activating transcription factor (ATF) sites in many cellular and viral promoters. The encoded protein represses Per1 and Per2 expression and therefore plays a role in the regulation of circadian rhythm. [provided by RefSeq, Feb 2014]