Pde3a (NM_018779) Mouse Tagged ORF Clone

CAT#: MG224945

Pde3a (GFP-tagged) - Mouse phosphodiesterase 3A cGMP inhibited (Pde3a), (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Pde3a
• Synonyms: A930022O17Rik; C87899
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG224945 representing NM_018779
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGTGCGGGGCGAGGCCGCCCAAGACCTAGCCAAGCCGGGCCTCGGAGGGGCGAGTCCCGCCCGTG
  TGGCAAGAGGGAACCACCGCCATCGTGGCGAGTCCTCTCCATCCCCTCGGGGCTCCGGCTGTTGTTGGAG
  AGCCCTGGCGCTGCAGCCGCTCCGGCGCTCGCCTCAACTCTCCTCGGCGCTGTGCGCGGGCTCCCTGTCC
  GTGCTGCTGGCGCTGCTGGTGAGGCTGGTCGGCGGGGAGGTCGGAGGCGAGCTGGAGAAGAGTCAGGAGG
  CGGCGGCCGAGGAGGAGGAGGAGGAAGGAGCCCGAGGGGGCGTCTTCCCGGGCCCTCGGGGAGGTGCTCC
  CGGGGGCGGCGCGCAGCTCAGCCCTTGGCTGCAGCCGGCCGCGCTGCTCTTCAGTCTCCTGTGCGCCTTC
  TTCTGGATGGGCTTGTGCCTGCTGCGCGCCGGGGTGCGCCTGCCTCTGGCCGTCGCGCTGTTGGCCGCCT
  GCTGCGCCGGGGAAGCGCTGGTGCAGCTCTCCTTGGGCGTGGGGGACGGTCGCCTGCTGTCGCTGCCCGC
  CGCGGGGGTCCTGCTCAGCTGCCTAGGTGGCGCGACATGGCTGGTGCTGAGGCTGAGGCTGGGCGTCCTC
  ATGGTCGCCTGGACTAGCGTGCTTAGGACGGTGGCCCTGGTTTCTCTGGAGAGGTTCAAGGTCGCCTGGA
  GACCTTACCTGGCATACTTGGCGGCCGTGCTTGGGCTGCTGCTGGCCAGGTACGCCGAGCAGATCCTGCC
  GCAGTGCTCCGGGCCGGCTCCGCCCAGGGAGCGGTTTGGGTCCCAGTTGAGCGCGAGGACCAAGGAAGAG
  ATTCCGGGGTGGAAGAGGAGGAGGCGGTCCAGCTCGGTGGTGGCCGGCGAGATGTCCGGCTGCAGTGGCA
  AGTCTCACCGGAGGACCTCCCTGCCCTGCATACCCAGGGAGCAGCTCATGGGCCATTCAGAATGGGACCA
  CAAGAGAGGGCCACGAGGATCCCAGTCAGGAACCAGCATCACTGTGGATATTGCTGTGATGGGTGAGGCC
  CATGGCCTCATTACCGACCTCCTGGCCGACCCTTCTCTGCCCCCGAATGTGTGTACATCCTTGAGGGCCG
  TAAGCAACTTGCTCAGCACCCAGCTCACCTTCCAGGCCATCCACAAGCCCAGAGTGAACCCCACCGTGAC
  CTTCAGTGAGAACTATACCTGCTCGGACTCTGAGGAAGGCTTGGAGAAAGACAAGCAGGCCATTTCCAAG
  CGCCTGAGAAGAAGTTTGCCTCCAGGCTTACTGAGAAGAGTCTCGTCAACTTGGACAACTACCACCTCTG
  CCACAGGTCTGCCCACCTTGGAGCCTGCGCCAGTGCGGAGGGACCGCAGTGCCAGCATAAAGCCGCACGA
  AGCCCCTTCACCCAGTGCTGTCAATCCTGACTCTTGGAATGCCCCAGGGTTGACGACTCTCACCAAAAGC
  AGATCCTTCACTTCATCCTATGCTGTCTCTGCAGCTAACCATGTAAAAGCTAAAAAGCAAAACAGGCCAG
  GTGGCCTTGCTAAAATTTCCCCCGTCCCCTCACCCTCCTCCTCGCCTCCCCAGGGATCACCTGCCAGCAG
  TCCTGTCAGCAACAGTGCTTCACAACAGTTCCCAGAATCCCCTGAAGTAACCATCAAGCGAGGGCCTGGA
  TCTCACAGGGCCTTAACTTACACCCAAAGTGCCCCTGACCTGTCCCCTCAGATCCCACCCCCGTCTGTCA
  TATGTAGCAGCTGTGGCAGACCTTATTCGCAAGGGAATCCTGCTGATGGACCCTCAGAGAGAAGTGGCCC
  AGCCATGCTGAAACCAAACAGAACAGATGACACTTCTCAAGTTACCTCCGATTATGAGACCAACAACAAC
  AGTGACAGCAGTGACATTCTGCAGAATGAAGAGGAAGCTGAGTGCCAGAGAGAACCCCAGAGGAAAGCAT
  CGGCTTGTGGCACCTATACTTCCCAGACCATGATCTTCCTGGACAAACCAATTCTTGCTCCAGAACCCCT
  GGTCATGGATAACCTGGACTCAATTATGGATCAGTTGAACACCTGGAATTTTCCAATTTTTGATTTAATG
  GAAAATATAGGAAGAAAATGTGGCCGTATTCTGAGCCAGGTATCATACAGACTGTTTGAAGACATGGGGC
  TCTTTGAAGCCTTTAAAATCCCGGTTAGGGAGTTTATGAATTACTTTCATGCTTTGGAGATCGGATACAG
  GGACATTCCTTATCATAACAGAATCCATGCCACCGATGTTCTACACGCTGTGTGGTATCTCACAACACAG
  CCGATTCCTGGCCTCCCAAGTGTGATTGGTGATCACGGCTCAGCAAGCGACTCCGATTCTGACAGTGGAT
  TTACACACGGACACATGGGATATGTGTTTTCAAAAATGTATCACGTGCCAGATGACAAATATGGATGCCT
  GTCTGGGAATATTCCAGCCCTAGAGTTGATGGCTCTGTACGTTGCTGCAGCCATGCATGACTACGATCAC
  CCAGGGAGGACAAATGCCTTCCTGGTTGCCACTAGTGCCCCTCAGGCGGTGCTATACAACGACCGTTCTG
  TTCTGGAGAACCATCACGCCGCTGCAGCCTGGAATCTCTTCATGTCCCGGCCAGAGTATAACTTCTTAGT
  TAACCTGGACCACGTGGAATTCAAGCACTTCCGCTTCCTAGTCATTGAAGCGATTTTGGCTACTGACCTG
  AAGAAACACTTTGACTTTGTAGCCAAATTTAACGCCAAGGTAAACGATGATGTCGGGATAGATTGGACCA
  ATGAAAACGACCGTCTCCTGGTGTGTCAGATGTGTATAAAGCTGGCTGACATCAACGGGCCTGCTAAATG
  CAAAGAACTCCACCTGCGGTGGACAGAGGGCATCGCCAGCGAGTTTTATGAGCAGGGCGATGAAGAAGCC
  AGCCTCGGCTTGCCCATAAGTCCCTTTATGGACCGCTCTGCCCCGCAGCTGGCCAATCTTCAGGAATCCT
  TTATCTCACACATCGTGGGTCCTCTCTGCCACTCCTATGACTCTGCGGGACTCATGCCAGGAAAATGGGT
  AGATGACAGTGATGATTCCGGAGACACCGATGACCCGGAAGAAGAGGAGGAAGAGGCTGAAACACCACAC
  GAGGACGAAGCCTGTGAAAGCAGTATAGCTCCCCGAAAGAAAAGTTTCAAAAGAAGGAGAATCTACTGCC
  AAATAACTCAGCACCTTCTGCAGAACCACATGATGTGGAAGAAAGTGATTGAAGAGGAACAGTGTTTATC
  AGGCACAGAGAACCAGTCCCTGGACCAGGTCCCTCTGCAGCATCCCTCAGAGCAGATCCAGGCTATCAAG
  GAGGAAGAGGAAGAGAAGGGGAAGCCAAGAGCAGAGGAGACCCTGGCTCCACAGCCAGACCTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG224945 representing NM_018779
  Red=Cloning site Green=Tags(s)
  
  MAVRGEAAQDLAKPGLGGASPARVARGNHRHRGESSPSPRGSGCCWRALALQPLRRSPQLSSALCAGSLS
  VLLALLVRLVGGEVGGELEKSQEAAAEEEEEEGARGGVFPGPRGGAPGGGAQLSPWLQPAALLFSLLCAF
  FWMGLCLLRAGVRLPLAVALLAACCAGEALVQLSLGVGDGRLLSLPAAGVLLSCLGGATWLVLRLRLGVL
  MVAWTSVLRTVALVSLERFKVAWRPYLAYLAAVLGLLLARYAEQILPQCSGPAPPRERFGSQLSARTKEE
  IPGWKRRRRSSSVVAGEMSGCSGKSHRRTSLPCIPREQLMGHSEWDHKRGPRGSQSGTSITVDIAVMGEA
  HGLITDLLADPSLPPNVCTSLRAVSNLLSTQLTFQAIHKPRVNPTVTFSENYTCSDSEEGLEKDKQAISK
  RLRRSLPPGLLRRVSSTWTTTTSATGLPTLEPAPVRRDRSASIKPHEAPSPSAVNPDSWNAPGLTTLTKS
  RSFTSSYAVSAANHVKAKKQNRPGGLAKISPVPSPSSSPPQGSPASSPVSNSASQQFPESPEVTIKRGPG
  SHRALTYTQSAPDLSPQIPPPSVICSSCGRPYSQGNPADGPSERSGPAMLKPNRTDDTSQVTSDYETNNN
  SDSSDILQNEEEAECQREPQRKASACGTYTSQTMIFLDKPILAPEPLVMDNLDSIMDQLNTWNFPIFDLM
  ENIGRKCGRILSQVSYRLFEDMGLFEAFKIPVREFMNYFHALEIGYRDIPYHNRIHATDVLHAVWYLTTQ
  PIPGLPSVIGDHGSASDSDSDSGFTHGHMGYVFSKMYHVPDDKYGCLSGNIPALELMALYVAAAMHDYDH
  PGRTNAFLVATSAPQAVLYNDRSVLENHHAAAAWNLFMSRPEYNFLVNLDHVEFKHFRFLVIEAILATDL
  KKHFDFVAKFNAKVNDDVGIDWTNENDRLLVCQMCIKLADINGPAKCKELHLRWTEGIASEFYEQGDEEA
  SLGLPISPFMDRSAPQLANLQESFISHIVGPLCHSYDSAGLMPGKWVDDSDDSGDTDDPEEEEEEAETPH
  EDEACESSIAPRKKSFKRRRIYCQITQHLLQNHMMWKKVIEEEQCLSGTENQSLDQVPLQHPSEQIQAIK
  EEEEEKGKPRAEETLAPQPDL
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018779
• ORF Size: 3423 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_018779.1, NP_061249.1
• RefSeq Size: 4205
• RefSeq ORF: 3426
• Locus ID: 54611