Peg10 (NM_001040611) Mouse Tagged ORF Clone

CAT#: MG225144

Peg10 (GFP-tagged) - Mouse paternally expressed 10 (Peg10) transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Peg10
• Synonyms: AA407948; Edr; HB-1; Mar2; Mart2; MEF3L; MyEF-3; Rtl2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG225144 representing NM_001040611
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGCTGCGGGTGGTTCCTCCAACTGCCCGCCCCCTCCCCCTCCCCCTCCTCCCAACAACAACAACA
  ACAACAACACCCCAAAGAGCCCAGGCGTGCCTGACGCCGAAGATGATGATGAACGCAGACACGATGAGCT
  CCCTGAAGACATCAACAACTTTGACGAAGACATGAACAGGCAGTTTGAGAATATGAACCTGCTGGATCAG
  GTGGAGTTGCTTGCACAGAGCTACAGTCTGCTGGATCATTTAGATGACTTTGATGATGATGATGAAGACG
  ATGACTTTGATCCAGAACCTGACCAGGATGAGCTCCCTGAGTACAGTGACGATGATGACCTGGAGCTTCA
  GGGTGCTGCAGCAGCCCCTATCCCAAACTTTTTCTCCGATGATGACTGCCTTGAAGACCTTCCTGAGAAG
  TTCGATGGCAACCCTGACATGCTGGGTCCTTTCATGTATCAGTGCCAGCTCTTCATGGAAAAGAGCACCA
  GAGATTTCTCAGTTGACCGCATCCGTGTGTGCTTCGTGACAAGCATGCTGATCGGCCGTGCCGCCCGCTG
  GGCTACTGCCAAGCTGCAAAGATGTACTTACCTGATGCACAACTACACTGCCTTTATGATGGAGCTGAAG
  CATGTCTTTGAAGACCCTCAGAGACGTGAAGCTGCCAAACGCAAGATCAGACGTCTGCGCCAGGGCCCTG
  GGCCTGTTGTGGACTACTCCAATGCATTCCAGATGATTGCCCAGGACCTGGATTGGACTGAGCCTGCCCT
  GATGGATCAGTTCCAGGAAGGTCTCAACCCAGACATTCGCGCAGAGCTGTCTCGCCAGGAGGCCCCCAAG
  ACCCTGGCTGCTCTGATTACTGCCTGTATTCACATCGAGAGAAGGCTGGCTCGTGACGCTGCTGCAAAGC
  CCGATCCTTCACCCAGAGCCTTGGTGATGCCTCCAAACAGCCAGACCGATCCCACCGAGCCTGTGGGAGG
  TGCCCGCATGCGCCTGTCCAAGGAAGAAAAGGAGAGACGCCGCAAAATGAATTTGTGTCTCTACTGTGGC
  AATGGAGGCCATTTCGCCGACACGTGTCCAGCGAAAGCCTCCAAGAATTCGCCGCCGGGAAACTCCCCGG
  CCCCGCTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG225144 representing NM_001040611
  Red=Cloning site Green=Tags(s)
  
  MAAAGGSSNCPPPPPPPPPNNNNNNNTPKSPGVPDAEDDDERRHDELPEDINNFDEDMNRQFENMNLLDQ
  VELLAQSYSLLDHLDDFDDDDEDDDFDPEPDQDELPEYSDDDDLELQGAAAAPIPNFFSDDDCLEDLPEK
  FDGNPDMLGPFMYQCQLFMEKSTRDFSVDRIRVCFVTSMLIGRAARWATAKLQRCTYLMHNYTAFMMELK
  HVFEDPQRREAAKRKIRRLRQGPGPVVDYSNAFQMIAQDLDWTEPALMDQFQEGLNPDIRAELSRQEAPK
  TLAALITACIHIERRLARDAAAKPDPSPRALVMPPNSQTDPTEPVGGARMRLSKEEKERRRKMNLCLYCG
  NGGHFADTCPAKASKNSPPGNSPAPL
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001040611
• ORF Size: 1128 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001040611.1, NP_001035701.1
• RefSeq Size: 6677
• RefSeq ORF: 1131
• Locus ID: 170676
• Gene Summary: This is a paternally expressed imprinted gene that is thought to have been derived from the Ty3/Gypsy family of retrotransposons. It contains two overlapping open reading frames, RF1 and RF2, and expresses two proteins: a shorter, gag-like protein (with a CCHC-type zinc finger domain) from RF1; and a longer, gag/pol-like fusion protein (with an additional aspartic protease motif) from RF1/RF2 by -1 translational frameshifting (-1 FS). While -1 FS has been observed in RNA viruses and transposons in both prokaryotes and eukaryotes, this gene represents the first example of -1 FS in a eukaryotic cellular gene. This gene is highly conserved across mammalian species and retains the heptanucleotide (GGGAAAC) and pseudoknot elements required for -1 FS. It is expressed in adult and embryonic tissues (most notably in placenta) and reported to have a role in cell proliferation, differentiation, apoptosis and cancer development. Knockout mice lacking this gene showed early embryonic lethality with placental defects, indicating the importance of this gene in embryonic development. [provided by RefSeq, Oct 2014]