Adam12 (NM_007400) Mouse Tagged ORF Clone

CAT#: MG225285

Adam12 (GFP-tagged) - Mouse a disintegrin and metallopeptidase domain 12 (meltrin alpha) (Adam12), (10ug)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Adam12
• Synonyms: Mltna
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG225285 representing NM_007400
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGAGCGCCCGGCGCGGCGCGCGCCCCCCGCCCGCGCCCTCCTGCTGGCCCTGGCTGGGGCCCTGC
  TGGCGCCCCGTGCAGCCCGAGGGATGAGTTTGTGGGACCAGAGAGGAACTTACGAAGTGGCCAGAGCCTC
  CCTTCTGAGCAAGGACCCTGGGATCCCAGGACAGAGCATCCCAGCCAAGGATCATCCAGACGTGCTGACT
  GTGCAACTGCAGCTGGAGAGCCGAGACCTGATCCTCAGCCTGGAAAGGAATGAGGGACTCATTGCCAATG
  GCTTCACGGAGACCCATTATCTGCAAGATGGTACTGATGTCTCTCTCACTCGAAATCACACGGATCATTG
  TTACTACCATGGACATGTGCAAGGAGATGCTGCATCAGTGGTCAGCCTCAGTACTTGCTCTGGTCTCCGG
  GGACTTATCATGTTTGAAAATAAAACGTACAGCTTAGAGCCAATGAAAAACACCACTGACAGCTACAAAC
  TCGTCCCAGCTGAGAGCATGACGAACATCCAAGGGCTGTGTGGGTCACAGCATAACAAGTCCAACCTCAC
  CATGGAAGATGTCTCCCCTGGAACCTCTCAAATGCGGGCAAGAAGGCATAAGAGAGAGACCCTTAAGATG
  ACCAAGTACGTAGAGCTGGTTATTGTGGCAGACAACAGAGAGTTTCAGAGGCAAGGAAAAGACCTGGAGA
  AAGTTAAGCAGCGATTAATAGAGATCGCCAATCACGTTGACAAGTTTTACAGACCACTGAACATCCGGAT
  CGTGCTGGTAGGAGTGGAAGTGTGGAATGACATCGACAAATGCTCTATAAGCCAGGACCCATTCACCAGC
  CTCCATGAGTTTCTAGACTGGAGAAAGATAAAGCTTCTACCTCGAAAATCCCACGACAATGCTCAGCTTA
  TCAGTGGGGTTTATTTCCAAGGAACCACCATCGGCATGGCACCCATCATGAGCATGTGCACTGCAGAACA
  GTCTGGAGGAGTTGTCATGGACCATTCAGACAGCCCCCTTGGTGCCGCAGTGACCTTGGCACATGAGCTG
  GGCCACAACTTCGGGATGAACCATGACACACTGGAGAGGGGCTGCAGCTGCAGAATGGCCGCAGAGAAAG
  GAGGCTGCATCATGAACCCGTCCACGGGGTTCCCATTCCCCATGGTGTTCAGCAGCTGCAGCAGGAAGGA
  CCTGGAGGCTAGCCTGGAGAAGGGCATGGGGATGTGCCTCTTCAACCTACCAGAGGTCAAGCAGGCCTTT
  GGGGGCCGGAAGTGTGGAAATGGCTATGTGGAAGAGGGAGAAGAGTGTGACTGCGGAGAACCGGAGGAAT
  GCACGAATCGCTGCTGTAACGCTACCACCTGTACTCTGAAGCCAGATGCTGTGTGCGCGCACGGGCAGTG
  CTGTGAAGACTGTCAGCTGAAGCCTCCAGGAACTGCATGCAGGGGCTCCAGCAACTCCTGTGACCTCCCA
  GAATTCTGCACAGGGACTGCCCCTCACTGTCCAGCCAATGTGTACCTACATGATGGCCACCCGTGTCAGG
  GCGTGGATGGTTACTGCTACAACGGCATCTGCCAGACCCATGAGCAGCAGTGTGTCACGCTCTGGGGACC
  AGGTGCTAAACCGGCTCCTGGCATCTGCTTTGAGCGAGTCAACTCTGCAGGAGATCCTTATGGTAACTGT
  GGCAAAGACTCCAAGAGCGCCTTCGCCAAATGTGAGCTGAGAGATGCCAAGTGTGGGAAAATCCAGTGTC
  AAGGTGGTGCAAGCCGACCTGTCATTGGTACCAATGCTGTTTCCATAGAAACAAATATCCCACAGCAGGA
  AGGAGGTCGGATTCTGTGCCGGGGGACCCATGTGTACTTGGGTGATGACATGCCAGACCCAGGGCTTGTG
  CTTGCAGGAACAAAGTGTGCAGAAGGAAAAATCTGCCTCAATCGTCGATGTCAGAATATCAGTGTCTTCG
  GCGTTCACAAGTGTGCCATGCAGTGCCACGGCCGAGGGGTATGTAACAACAGGAAGAATTGCCACTGTGA
  AGCCCACTGGGCTCCACCCTTCTGTGACAAGTTTGGCTTTGGAGGAAGCACAGACAGTGGTCCCATCAGG
  CAAGCAGATAACCAGGGCTTGACTGTAGGAATCCTGGTGAGCATCCTGTGTCTGCTTGCTGCTGGATTTG
  TGGTGTATCTCAAAAGGAAGACGTTGATGCGGCTGCTGTTCACACATAAAAAAACCACCATGGAAAAGCT
  AAGGTGTGTGCACCCTTCCCGGACACCCAGTGGCCCTCACCTTGGCCAGGCTCACCACACCCCCGGGAAA
  GGCCTGCTGATGAACCGGGCACCACATTTCAATACCCCCAAGGACAGGCACTCGCTGAAATGCCAGAACA
  TGGACATCAGCAGGCCCCTCGACGCTCGAGCCGTCCCACAGCTTCAGTCACCTCAGCGAGTGCTCCTGCC
  TCTCCACCAGACCCCACGTGCACCCAGTGGCCCTGCCAGGCCCCTGCCCGCCAGTCCTGCAGTCAGGCAG
  GCCCAGGGCATTCGAAAACCCAGTCCTCCTCAGAAGCCTCTGCCTGCTGATCCACTGAGCAGGACTTCTC
  GGCTCACTAGTGCCTTGGTGAGGACCCCAGGGCAGCAGGAACCTGGGCACCGCCCAGCCCCCATCAGACC
  TGCCCCTAAGCATCAAGTACCCAGACCTTCCCACAATGCCTATATCAAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG225285 representing NM_007400
  Red=Cloning site Green=Tags(s)
  
  MAERPARRAPPARALLLALAGALLAPRAARGMSLWDQRGTYEVARASLLSKDPGIPGQSIPAKDHPDVLT
  VQLQLESRDLILSLERNEGLIANGFTETHYLQDGTDVSLTRNHTDHCYYHGHVQGDAASVVSLSTCSGLR
  GLIMFENKTYSLEPMKNTTDSYKLVPAESMTNIQGLCGSQHNKSNLTMEDVSPGTSQMRARRHKRETLKM
  TKYVELVIVADNREFQRQGKDLEKVKQRLIEIANHVDKFYRPLNIRIVLVGVEVWNDIDKCSISQDPFTS
  LHEFLDWRKIKLLPRKSHDNAQLISGVYFQGTTIGMAPIMSMCTAEQSGGVVMDHSDSPLGAAVTLAHEL
  GHNFGMNHDTLERGCSCRMAAEKGGCIMNPSTGFPFPMVFSSCSRKDLEASLEKGMGMCLFNLPEVKQAF
  GGRKCGNGYVEEGEECDCGEPEECTNRCCNATTCTLKPDAVCAHGQCCEDCQLKPPGTACRGSSNSCDLP
  EFCTGTAPHCPANVYLHDGHPCQGVDGYCYNGICQTHEQQCVTLWGPGAKPAPGICFERVNSAGDPYGNC
  GKDSKSAFAKCELRDAKCGKIQCQGGASRPVIGTNAVSIETNIPQQEGGRILCRGTHVYLGDDMPDPGLV
  LAGTKCAEGKICLNRRCQNISVFGVHKCAMQCHGRGVCNNRKNCHCEAHWAPPFCDKFGFGGSTDSGPIR
  QADNQGLTVGILVSILCLLAAGFVVYLKRKTLMRLLFTHKKTTMEKLRCVHPSRTPSGPHLGQAHHTPGK
  GLLMNRAPHFNTPKDRHSLKCQNMDISRPLDARAVPQLQSPQRVLLPLHQTPRAPSGPARPLPASPAVRQ
  AQGIRKPSPPQKPLPADPLSRTSRLTSALVRTPGQQEPGHRPAPIRPAPKHQVPRPSHNAYIK
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007400
• ORF Size: 2709 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_007400.2, NP_031426.2
• RefSeq Size: 7675
• RefSeq ORF: 2712
• Locus ID: 11489
• Gene Summary: This gene encodes a member of a disintegrin and metalloprotease (ADAM) family of endoproteases that play important roles in various biological processes including cell signaling, adhesion and migration. The encoded preproprotein undergoes proteolytic processing to generate a mature, functional protein that localizes to the cell surface. About a third of the mice lacking the encoded protein die before weaning. Overexpression of the encoded protein in a mouse model of Duchenne muscular dystrophy alleviates the muscle pathology by preventing cell necrosis and inflammation. [provided by RefSeq, May 2016]