Fabp7 (NM_021272) Mouse Tagged ORF Clone

CAT#: MR200772

Fabp7 (Myc-DDK-tagged) - Mouse fatty acid binding protein 7, brain (Fabp7)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Fabp7
• Synonyms: B-FABP; BFABP; Blbp; MRG
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR200772 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTAGATGCTTTCTGCGCAACCTGGAAGCTGACAGACAGTCAGAATTTTGATGAGTACATGAAAGCTC
  TGGGCGTGGGCTTTGCCACTAGGCAAGTGGGAAACGTGACCAAACCAACTGTGATTATCAGTCAGGAAGG
  TGGCAAAGTGGTGATCCGGACACAATGCACATTCAAGAACACAGAGATCAATTTCCAGCTGGGAGAAGAG
  TTTGAAGAAACCAGCATAGATGACAGAAACTGTAAGTCTGTGGTTCGGTTGGATGGAGACAAGCTCATTC
  ATGTGCAGAAGTGGGATGGCAAAGAAACAAATTGTACCAGAGAAATTAAGGATGGCAAGATGGTCGTGAC
  TCTTACCTTTGGGGATATCGTTGCTGTTCGCTGTTATGAAAAGGCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR200772 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MVDAFCATWKLTDSQNFDEYMKALGVGFATRQVGNVTKPTVIISQEGGKVVIRTQCTFKNTEINFQLGEE
  FEETSIDDRNCKSVVRLDGDKLIHVQKWDGKETNCTREIKDGKMVVTLTFGDIVAVRCYEKA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021272
• ORF Size: 399 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021272.1, NM_021272.2, NM_021272.3, NP_067247.1
• RefSeq Size: 762 bp
• RefSeq ORF: 399 bp
• Locus ID: 12140
• MW: 14.9 kDa
• Gene Summary: B-FABP could be involved in the transport of a so far unknown hydrophobic ligand with potential morphogenic activity during CNS development. It is required for the establishment of the radial glial fiber system in developing brain, a system that is necessary for the migration of immature neurons to establish cortical layers. [UniProtKB/Swiss-Prot Function]