Mrpl43 (NM_053164) Mouse Tagged ORF Clone

CAT#: MR201223

Mrpl43 (Myc-DDK-tagged) - Mouse mitochondrial ribosomal protein L43 (Mrpl43), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Mrpl43
• Synonyms: 4930442D21Rik; bMRP36a
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR201223 representing NM_053164
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCGGTCGCGGGACTTCGAGCCGCTTTCTGACCAGCGTCCTCCATAATGGGCTAGGGCGTTACGTGC
  AGCAGCTGCAGCGCCTCAGCCTCAGCCTCAGCCGGGATGCGCCCTCGTCCCGCGGCGCCAGGGAGTTCGT
  GGAACGCGAGGTGACCGACTTCGCCCGACGGAACCCAGGAGTCGTAGTATACGTGAACCCGCGCCCGTGC
  GCCATGCCACGAATAGTGGCCGAATACCTTAATGGGGCTGTGCGCGAGGAAAACGTCAACAGCAAGTCGG
  TGGAGGAGATCAAGTCGTTGGTGCAGAAGCTGGCAGACCAGTCCGGCTTGGATGTGATCCGCATCCGCAA
  GCCCTTCCACACGGACAACCCGAGCATCCAGGGCCAGTGGCACCCCTTCACCAACAAACGGACTGCCCTC
  CACGGGCTGCGGCCCAGAGAACTCCGGGATTCTGCTCCAGCTTCGATGCAAGCACAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR201223 representing NM_053164
  Red=Cloning site Green=Tags(s)
  
  MTGRGTSSRFLTSVLHNGLGRYVQQLQRLSLSLSRDAPSSRGAREFVEREVTDFARRNPGVVVYVNPRPC
  AMPRIVAEYLNGAVREENVNSKSVEEIKSLVQKLADQSGLDVIRIRKPFHTDNPSIQGQWHPFTNKRTAL
  HGLRPRELRDSAPASMQAQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_053164
• ORF Size: 477 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_053164.1, NM_053164.2, NM_053164.3, NP_444394.1
• RefSeq Size: 1213
• RefSeq ORF: 480
• Locus ID: 94067
• MW: 17.9 kDa
• Gene Summary: Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. [provided by RefSeq, Jul 2008]