Timm50 (BC010303) Mouse Tagged ORF Clone

CAT#: MR202741

Timm50 (Myc-DDK-tagged) - Mouse translocase of inner mitochondrial membrane 50 homolog (yeast) (cDNA clone MGC:11857 IMAGE:3597676)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Timm50
• Synonyms: TIM50L
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR202741 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATCATCGAGCCCACCAGTCCCTGCCTTCTCCCAGACCCTCTGCGGGAGCCCTACTACCAGCCACCTT
  ATACACTGGTCCTGGAGCTTACCGGGGTCCTTCTGCACCCAGAGTGGTCGCTGGCCACTGGCTGGAGGTT
  TAAGAAGCGTCCAGGCATCGAGACCTTGTTCCAGCAGCTTGCCCCTCTGTATGAAATAGTCATCTTCACA
  TCAGAGACTGGCATGACTGCATTTCCACTCATTGATAGTGTGGACCCTCACGGTTTCATCTCCTACCGTC
  TGTTCCGGGACGCCACCAGATATATGGAGGGACATCATGTGAAGGACATCTCCTGTCTGAATCGGGACCC
  AGCCCGAGTAGTAGTCGTGGACTGCAAGAAGGAAGCTTTCCGCCTCCAGCCCTTCAACGGTGTTGCCCTG
  CGGCCCTGGGATGGCAACTCAGATGACCGGGTCTTGCTGGACCTGTCTGCCTTCCTCAAGACCATTGCAC
  TGAACCAAGTGGAGGACGTCCGGACGGTGCTGGAGCACTATGCTCTAGAGGATGACCCACTGGAGGCATT
  CAAACAGCGGCAGAGCCGGCTTGAGCAGGAGGAGCAGCAGCGCCTGGCCGAGCTCTCCAAGTCCAACAGG
  CAGGGCCTCTCCTTTGGCTCACTCGCCAGCCGACTGTGGCCTCGCTCCAAGCAGCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR202741 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MIIEPTSPCLLPDPLREPYYQPPYTLVLELTGVLLHPEWSLATGWRFKKRPGIETLFQQLAPLYEIVIFT
  SETGMTAFPLIDSVDPHGFISYRLFRDATRYMEGHHVKDISCLNRDPARVVVVDCKKEAFRLQPFNGVAL
  RPWDGNSDDRVLLDLSAFLKTIALNQVEDVRTVLEHYALEDDPLEAFKQRQSRLEQEEQQRLAELSKSNR
  QGLSFGSLASRLWPRSKQP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC010303
• ORF Size: 687 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC010303, AAH10303
• RefSeq Size: 1487 bp
• RefSeq ORF: 689 bp
• Locus ID: 66525
• MW: 26.4 kDa