Pdgfd (BC030896) Mouse Tagged ORF Clone

CAT#: MR203421

Pdgfd (Myc-DDK-tagged) - Mouse platelet-derived growth factor, D polypeptide (cDNA clone MGC:31518 IMAGE:4489485)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pdgfd
• Synonyms: 1110003I09Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR203421 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAACGGCTCGTTTTAGTCTCCATTCTCCTGTGCGCGAACTTTAGCTGCTATCCGGACACTTTTGCGA
  CTCCGCAGAGAGCATCCATCAAAGCTTTGCGCAATGCCAACCTCAGGAGAGATGAGAGCAATCACCTCAC
  AGACTTGTACCAGAGAGAGGAGAACATTCAGGTGACAAGCAATGGCCATGTGCAGAGTCCTCGCTTCCCG
  AACAGCTACCCAAGGAACCTGCTTCTGACATGGTGGCTCCGTTCCCAGGAGAAAACACGGATACAACTGT
  CCTTTGACCATCAATTCGGACTAGAGGAAGCAGAAAATGACATTTGTAGGTATGACTTTGTGGAAGTTGA
  AGAAGTCTCAGAGAGCAGCACTGTTGTCAGAGGAAGATGGTGTGGCCACAAGGAGATCCCTCCAAGGATA
  ACGTCAAGAACAAACCAGATTAAAATCACATTTAAGTCTGATGACTACTTTGTGGCAAAACCTGGATTCA
  AGATTTATTATTCATTTGTGGAAGATTCCCAACCGGAAGCAGCCTCAGAGACCAACTGGGAATCAGTCAC
  AAGCTCTTTCTCTGGGGTGTCCTATCACTCTCCATCAATAACGGACCCCACTCTCACTGCTGATGCCCTG
  GACAAAACTGTCGCAGAATTCGATACCGTGGAAGATCTACTTAAGCACTTCAATCCAGTGTCTTGGCAAG
  ATGATCTGGAGAATTTGTATCTGGACACCCCTCATTATAGAGGCAGGTCATACCATGATCGGAAGTCCAA
  AGGTATTGAAGTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR203421 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MQRLVLVSILLCANFSCYPDTFATPQRASIKALRNANLRRDESNHLTDLYQREENIQVTSNGHVQSPRFP
  NSYPRNLLLTWWLRSQEKTRIQLSFDHQFGLEEAENDICRYDFVEVEEVSESSTVVRGRWCGHKEIPPRI
  TSRTNQIKITFKSDDYFVAKPGFKIYYSFVEDSQPEAASETNWESVTSSFSGVSYHSPSITDPTLTADAL
  DKTVAEFDTVEDLLKHFNPVSWQDDLENLYLDTPHYRGRSYHDRKSKGIEV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC030896
• ORF Size: 783 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC030896, AAH30896
• RefSeq Size: 2870 bp
• RefSeq ORF: 785 bp
• Locus ID: 71785
• MW: 30.2 kDa