Sntb1 (BC003748) Mouse Tagged ORF Clone

CAT#: MR203713

Sntb1 (Myc-DDK-tagged) - Mouse syntrophin, basic 1 (cDNA clone MGC:5857 IMAGE:3600695)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Sntb1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR203713 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGTAGCGGCAGCGGCTGTGGCTGCGCCGGCTGGCGGTGGAGGCGCCCGGGCGCAGCGGAGCGGAC
  TGCTGGAAGTTTTGGTGCGGGATCGCTGGCATAAAGTTCTGGTGAACTTGAGCGAGGACGCCCTGGTGTT
  GAGCTGCGAGGAGGGCGCTGCGGCGTACAACGGCATAGGGGCTGCCACCAATGGTTCGTTCTGCAGGGGC
  TCCGGGACCGGGCACCCGGTCCCCGGTGTCGCTCAAGCCCCGGACTCTCCCGCCGGGGTCCGCACCGCCT
  TCACCGACCTCCCGGAGCAGGTACCTGAGTCCATCTCAAACCAGAAGAGGGGCGTGAAGGTGCTGAAGCA
  GGAGCTGGGCGGGCTGGGCATTAGCATCAAAGGGGGCAAGGAAAACAAGATGCCCATACTCATCAGCAAG
  ATCTTCAAGGGGCTGGCAGCGGATCAGACCCAAGCCCTGTATGTGGGCGATGCCATCCTGTCGGTGAACG
  GAGCAGACCTGCGGGACGCCACCCACGACGAGGCAGTGCAGGCATTGAAGCGCGCAGGCAAGGAAGTGCT
  GCTGGAAGTGAAGTATATGAGAGAAGCCACACCTTACGTGAAGAAAGGGTCCCCGGTGTCTGAGATTGGA
  TGGGAAACACCTCCACCTGAGTCTCCAAGATTAGGGGGTGGCTCTGCAGAGCCTCTGTCATCCCAGTCCT
  TCTCCTTCCATAGAGACCGGAAAAGCATCCCCCTCAAGATGTGTTATGTCACCCGGAACATGACCTTGGC
  TGACCCTGAAAACAGCACCCATCCTAGTGACCTCATACCAATACAAGGATGGGCTGCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR203713 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAVAAAAVAAPAGGGGARAQRSGLLEVLVRDRWHKVLVNLSEDALVLSCEEGAAAYNGIGAATNGSFCRG
  SGTGHPVPGVAQAPDSPAGVRTAFTDLPEQVPESISNQKRGVKVLKQELGGLGISIKGGKENKMPILISK
  IFKGLAADQTQALYVGDAILSVNGADLRDATHDEAVQALKRAGKEVLLEVKYMREATPYVKKGSPVSEIG
  WETPPPESPRLGGGSAEPLSSQSFSFHRDRKSIPLKMCYVTRNMTLADPENSTHPSDLIPIQGWAA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC003748
• ORF Size: 828 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC003748, AAH03748
• RefSeq Size: 1239 bp
• RefSeq ORF: 830 bp
• Locus ID: 20649
• MW: 28.9 kDa
• Gene Summary: Adapter protein that binds to and probably organizes the subcellular localization of a variety of membrane proteins. May link various receptors to the actin cytoskeleton and the dystrophin glycoprotein complex. [UniProtKB/Swiss-Prot Function]