Eif4b (BC007171) Mouse Tagged ORF Clone

CAT#: MR205574

  • TrueORF®

Eif4b (Myc-DDK-tagged) - Mouse eukaryotic translation initiation factor 4B (cDNA clone MGC:7530 IMAGE:3492114)


  "BC007171" in other vectors (4)


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Reconstitution Protocol

USD 220.00

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Size
    • 10 ug

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Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Symbol Eif4b
Synonyms Eif4a2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR205574 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACCGCTATGGGGGCCGGGATCGCTATGATGACCGAGGCAGCAGAGACTATGACCGAGGCTATGACT
CCAGGATAGGCAGTGGCAGAAGAGCATTTGGAAGTGGGTACCGGAGAGATGATGACTACAGAGGAGGTGG
GGACCGCTATGAAGACCGCTATGACAGACGGGATGATCGGTCGTGGAGCTCCAGGGATGACTACTCTCGG
GATGATTATAGGCGTGATGACAGAGGTCCCCCCCAGAGACCCAGACTGAACCTCAAGCCTCGCAGCGCTC
CTAAGGAGGATGACGCCTCCGCCAGCACCTCCCAGTCCAGCCGGGCAGCCTCCATCTTTGGAGGGGCGAA
GCCTGTTGACACAGCTGCTAGGGAAAGAGAAGTAGAGGAGCGGCTACAGAAGGAGCAGGAGAAGCTGCAG
CGTCAGCTGGATGAGCCAAAACTAGACCGCCGGCCCCGGGAGAGACACCCAAGCTGGCGAAGTGAAGAAA
CTCAGGAAAGAGAACGGTCAAGGACAGGAAGTGAGTCATCGCAGACTGGGGCCTCAGCCACATCTGGCAG
AAATACACGAAGGAGAGAGAGTGAGAAGTCTCTAGAAAATGAAACCCTCAATAAAGAAGAAGACTGTCAC
TCTCCAACCTCTAAGCCTCCTAAACCTGACCAGCCTCTAAAGGTAATGCCAGCCCCTCCACCAAAGGAGA
ATGCGTGGGTGAAGCGAAGCTCTAACCCTCCTGCCCGATCTCAGAGCTCAGACACAGAGCAGCCGTCCCC
TACAAGTGGTGGAGGGAAAGTAGCTGCAGTCCAGCCCCCTGAGGAAGGACCATCAAGAAAAGATGGAAAT
AAAGTGGATGTGGTGGGTGCCACACAAGGCCAAGCTGGAAGCTGCAGCCGTGGTCCCGGGGATGGAGGGA
GCAGAGACCACTGGAAGGACTTGGATAGGAAGGATGGCAAAAAAGATCAAGATTCCAGATCTGCACCTGA
GCCAAAGAAACCTGAGGAGAACCCAGCCTCCAAGTTCAGCTCTGCAAGCAAGTACGCTGCTCTGTCTGTG
GATGGCGAGGATGAGGATGAGGGCGACGACTGCACTGAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR205574 protein sequence
Red=Cloning site Green=Tags(s)

MDRYGGRDRYDDRGSRDYDRGYDSRIGSGRRAFGSGYRRDDDYRGGGDRYEDRYDRRDDRSWSSRDDYSR
DDYRRDDRGPPQRPRLNLKPRSAPKEDDASASTSQSSRAASIFGGAKPVDTAAREREVEERLQKEQEKLQ
RQLDEPKLDRRPRERHPSWRSEETQERERSRTGSESSQTGASATSGRNTRRRESEKSLENETLNKEEDCH
SPTSKPPKPDQPLKVMPAPPPKENAWVKRSSNPPARSQSSDTEQPSPTSGGGKVAAVQPPEEGPSRKDGN
KVDVVGATQGQAGSCSRGPGDGGSRDHWKDLDRKDGKKDQDSRSAPEPKKPEENPASKFSSASKYAALSV
DGEDEDEGDDCTE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC007171
ORF Size 1089 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq BC007171, AAH07171
RefSeq Size 3658 bp
RefSeq ORF 1091 bp
Locus ID 75705
MW 40.7 kDa

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