Dmtf1 (BC045141) Mouse Tagged ORF Clone

CAT#: MR206436

Dmtf1 (Myc-DDK-tagged) - Mouse cyclin D binding myb-like transcription factor 1 (cDNA clone MGC:54617 IMAGE:6307203)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Dmtf1
• Synonyms: Dimp, Dmp1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR206436 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCACAGTTGAAGAGGATTCTGACACAGTAACAGTAGAAACTGTGAACTCTGTGACGTTTACTCAGG
  ACACGGACGGGAATCTCATTCTTCATTGCCCTCAGAATGTTTCAGAAAATGATCAGAGCTTTGAGGTGAC
  CATGACGGCAACTACAGAGGTGGCAGATGATGAACTTTCTGAGGGAACTGTGACACAAATTCAGATTTTA
  CAGAATGATCAACTAGATGAAATATCTCCATTGGGTACTGAGGAAGTCTCAGCAGTTAGCCAAGCGTGGT
  TTACAACTAAAGAAGATAAGGATTCTCTCACTAACAAAGGACATAAATGGAAGCAGGGGATGTGGTCCAA
  GGAAGAAATTGATATTTTAATGAACAACATCGAGCGCTATCTGAAGGCTCGCGGAATAAAAGATGCTACA
  GAAATCATCTTTGAGATGTCAAAAGACGAAAGAAAAGATTTCTACAGGACTATAGCGTGGGGGCTGAACC
  GGCCTTTGTTTGCAGTTTATAGAAGAGTGCTGCGCATGTATGATGACAGGAACCATGTGGGAAAATACAC
  TCCTGAAGAGATCGAGAAGCTCAAGGAGCTCCGGATAAAACACGGCAATGACTGGGCAACAATAGGGGCG
  GCCCTAGGAAGAAGTGCCTCTTCTGTCAAAGACCGCTGCCGGCTGATGAAGGATACCTGCAACACAGGGA
  AATGGACAGAAGAAGAAGAAAAGAGACTTGCAGAGGTAGTTCATGAATTAACAAGCACGGAGCCAGGTGA
  CATCGTCACACAGGGTGTGTCTTGGGCAGCTGTAGCTGAAAGAGTGGGTACCCGCTCAGAAAAGCAATGC
  CGTTCTAAATGGCTCAACTACCTGAACTGGAAGCAGAGTGGGGGTACTGAATGGACCAAGGAAGATGAAA
  TCAATCTCATCCTAAGGATAGCTGAGCTTGATGTGGCCGATGAAAATGACATAAACTGGGATCTTTTAGC
  TGAAGGATGGAGCAGTGTCCGTTCACCACAGTGGCTTCGAAGTAAATGGTGGACCATCAAAAGGCAAATT
  GCAAACCATAAGGATGTTTCATTTCCTGTGTACAGCATGTTCAGATCAGAGTCGCCCGCTTGGAAGATAA
  TACAGCCATCTCTCCAAGCCCCATGGCAGCGTTGCAGATTCCAGTCCAGATCACCCACGTCTCTTCAACA
  GACTCCCCTGCTGCTTCTGTTGACTCAGAAACAATCACAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR206436 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSTVEEDSDTVTVETVNSVTFTQDTDGNLILHCPQNVSENDQSFEVTMTATTEVADDELSEGTVTQIQIL
  QNDQLDEISPLGTEEVSAVSQAWFTTKEDKDSLTNKGHKWKQGMWSKEEIDILMNNIERYLKARGIKDAT
  EIIFEMSKDERKDFYRTIAWGLNRPLFAVYRRVLRMYDDRNHVGKYTPEEIEKLKELRIKHGNDWATIGA
  ALGRSASSVKDRCRLMKDTCNTGKWTEEEEKRLAEVVHELTSTEPGDIVTQGVSWAAVAERVGTRSEKQC
  RSKWLNYLNWKQSGGTEWTKEDEINLILRIAELDVADENDINWDLLAEGWSSVRSPQWLRSKWWTIKRQI
  ANHKDVSFPVYSMFRSESPAWKIIQPSLQAPWQRCRFQSRSPTSLQQTPLLLLLTQKQSH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC045141
• ORF Size: 1230 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC045141, AAH45141
• RefSeq Size: 3510 bp
• RefSeq ORF: 1232 bp
• Locus ID: 23857
• MW: 47.3 kDa