Crbn (BC086488) Mouse Tagged ORF Clone

CAT#: MR206875

Crbn (Myc-DDK-tagged) - Mouse cereblon (cDNA clone MGC:103113 IMAGE:30443656)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Crbn
• Synonyms: piL
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR206875 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAAACCACCTGCCGCTTCTGCCTGACAGTGAAGATGAAGATGATGAAATTGAAATGGAAGTTGAAG
  ACCAAGATAGTAAAGAAGCCAGAAAACCGAATATCATAAACTTTGACACCAGTCTGCCAACCTCACATAC
  ATACCTGGGAGCTGATATGGAGGAGTTCCACGGGAGAACTTTGCATGACGACGACAGCTGCCAGGTGATC
  CCAGTCCTTCCTGAGGTGCTGATGATCCTGATTCCTGGGCAGACACTCCCACTGCAGCTCTCTCACCCAC
  AGGAAGTCAGCATGGTGCGGAACTTAATCCAGAAAGACAGGACCTTTGCAGTCCTTGCATACAGTAATGT
  GCAAGAAAGGGAAGCACAGTTTGGGACAACAGCAGAGATCTATGCCTATCGAGAAGAGCAGGAGTTTGGA
  ATTGAAGTAGTGAAAGTGAAAGCAATTGGAAGGCAGCGGTTCAAGGTCCTCGAACTTCGAACACAGTCAG
  ATGGAATCCAGCAAGCTAAAGTGCAGATTTTGCCAGAGTGTGTGTTGCCGTCAACCATGTCTGCAGTGCA
  GGTAGAATCACTCAATAAGTGCCAGGTATTTCCTTCAAAACCCATCTCCTGGGAAGACCAGTATTCATGT
  AAATGGTGGCAGAAATACCAGAAGAGAAAGTTTCACTGTGCAAATCTAACATCATGGCCTCGCTGGCTGT
  ATTCATTATATGATGCTGAAACATTAATGGATAGAATTAAGAAACAGCTACGTGAATGGGATGAAAATCT
  CAAAGATGATTCTCTTCCTGAAAATCCAATAGACTTTTCTTACAGAGTAGCTGCTTGTCTTCCTATTGAT
  GATGTATTGAGAATTCAGCTCCTTAAAATCGGCAGTGCTATTCAACGGCTTCGCTGTGAATTGGACATCA
  TGAACAAATGTACTTCCCTTTGCTGTAAACAATGTCAAGAAACAGAAATAACGACAAAGAATGAAATATT
  TAGTTTATCCTTATGTGGTCCAATGGCAGCATATGTGAATCCTCATGGATATGTACATGAGACACTGACT
  GTGTATAAAGCGTCCAACCTGAATCTGATAGGCCGGCCTTCTACAGTGCACAGCTGGTTTCCCGGGTATG
  CATGGACCATTGCCCAGTGCAAGATCTGTGCAAGCCATATTGGATGGAAATTTACAGCCACAAAAAAAGA
  CATGTCACCTCAAAAATTTTGGGGCTTAACTCGCTCTGCTCTGTTACCCACAATTCCAGAGACTGAAGAT
  GAAATAAGTCCAGACAAAGTAATACTTTGTTTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR206875 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGNHLPLLPDSEDEDDEIEMEVEDQDSKEARKPNIINFDTSLPTSHTYLGADMEEFHGRTLHDDDSCQVI
  PVLPEVLMILIPGQTLPLQLSHPQEVSMVRNLIQKDRTFAVLAYSNVQEREAQFGTTAEIYAYREEQEFG
  IEVVKVKAIGRQRFKVLELRTQSDGIQQAKVQILPECVLPSTMSAVQVESLNKCQVFPSKPISWEDQYSC
  KWWQKYQKRKFHCANLTSWPRWLYSLYDAETLMDRIKKQLREWDENLKDDSLPENPIDFSYRVAACLPID
  DVLRIQLLKIGSAIQRLRCELDIMNKCTSLCCKQCQETEITTKNEIFSLSLCGPMAAYVNPHGYVHETLT
  VYKASNLNLIGRPSTVHSWFPGYAWTIAQCKICASHIGWKFTATKKDMSPQKFWGLTRSALLPTIPETED
  EISPDKVILCL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC086488
• ORF Size: 1293 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC086488, AAH86488
• RefSeq Size: 2107
• RefSeq ORF: 1295
• Locus ID: 58799
• MW: 49.5 kDa
• Gene Summary: This gene encodes a protein with a Lon protease domain, a "regulators of G protein-signaling" (RGS)-like domain and a leucine zipper. It has been proposed to regulate the assembly and surface expression of large-conductance calcium-activated potassium channels in brain and to bind thalidomide. In humans mutation in this gene causes autosomal recessive nonsyndromic cognitive disability. In mouse deficiency of this gene serves as a model to study the molecular mechanisms governing learning and memory as they relate to intellectual disability. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Jan 2013]