Nlrp6 (BC031139) Mouse Tagged ORF Clone

CAT#: MR207117

Nlrp6 (Myc-DDK-tagged) - Mouse NLR family, pyrin domain containing 6 (cDNA clone MGC:37241 IMAGE:4972485)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Nlrp6
• Synonyms: AI504961|Avr|Nalp6|Navr|Navr; Avr|Non-AVR|Pypaf5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR207117 representing BC031139
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGAAGTCTGCAGGCACCAATGGACCCCGGGTTCAGGGAGAGCTGAGAACGCTGTGTCGCCTGGCCA
  GGGAGGGCATCCTGGATCATCATAAAGCACAATTCTCAGAAGAGGACCTGGAGAAATTGAAGCTTCGGGG
  TTCCCAAGTTCAGACAATATTTCTCAACAAGAAAGAGATACCAGGAGTGCTAAAAACTGAGGTCACCTAC
  CAGTTCATTGACCAGAGCTTCCAGGAGTTCTTGGCTGCATTGTCATACCTACTAGAAGCTGAGCGAACTC
  CGGGGACGCCCGCAGGCGGTGTGCAGAAGCTCCTGAACTCTGACGCGGAGCTACGTGGTCATCTTGCACT
  GACCACCCGATTCCTCTTTGGCCTGCTAAATACAGAGGGGCTTCGTGACATTGGAAACCATTTTGGCTGT
  GTGGTGCCAGATCATGTGAAAAAGGACACCCTGCGGTGGGTACAAGGACAGAGCCACCCCAAGGGGCCAC
  CAGTAGGGGCAAAAAAGACTGCTGAGCTGGAGGACATCGAGGACGCAGAGGAGGAGGAGGAGGAAGAGGA
  GGACCTCAATTTTGGACTGGAGCTGCTGTACTGCCTGTATGAGACACAGGAGGAGGATTTTGTTCGACAG
  GCTCTCAGCAGCCTTCCAGAGATAGTACTGGAGCGAGTCAGGTTGACCCGCATGGACCTTGAAGTTCTGA
  ATTACTGCGTGCAGTGCTGCCCAGATGGCCAGGCTCTGAGACTGGTGAGCTGTGGGCTGGTGGCGGCAAA
  GGAGAAGAAGAAGAAGAAGAAGAGCCTGGTGAAGCGGCTGAAGGGCTCTCAAAGCACCAAGAAACAACCC
  CCAGTCTCCTTGCTGCGTCCACTCTGTGAGACAATGACTACCCCGAAATGTCATCTGAGTGTTCTGATCT
  TGTCACACTGCAGACTCCCTGATGCAGTTTGCCGAGACCTTTCCGAGGCCCTGAAGGTAGCTCCTGCCCT
  AAGGGAGTTGGGCCTCCTCCAGAGCCGGCTCACCAACACAGGCCTGCGTTTACTGTGCGAAGGCCTGGCT
  TGGCCCAAGTGCCAGGTGAAGACACTCAGGATGCAGCTGCCTGACCTCCAAGAGGTGATCAATTACTTGG
  TCATCGTGCTCCAGCAGAGCCCAGTCCTGACCACTCTGGACCTCAGTGGCTGTCAGCTGCCTGGCGTCAT
  TGTGGAACCTCTGTGTGCAGCCCTGAAGCACCCTAAATGCTCCCTAAAGACCCTCAGTCTGACCTCTGTG
  GAGCTGAGTGAGAACTCACTGAGGGATCTTCAAGCTGTGAAGACATCAAAGCCAGATCTGTCCATCATAT
  ATTCAAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR207117 representing BC031139
  Red=Cloning site Green=Tags(s)
  
  MLKSAGTNGPRVQGELRTLCRLAREGILDHHKAQFSEEDLEKLKLRGSQVQTIFLNKKEIPGVLKTEVTY
  QFIDQSFQEFLAALSYLLEAERTPGTPAGGVQKLLNSDAELRGHLALTTRFLFGLLNTEGLRDIGNHFGC
  VVPDHVKKDTLRWVQGQSHPKGPPVGAKKTAELEDIEDAEEEEEEEEDLNFGLELLYCLYETQEEDFVRQ
  ALSSLPEIVLERVRLTRMDLEVLNYCVQCCPDGQALRLVSCGLVAAKEKKKKKKSLVKRLKGSQSTKKQP
  PVSLLRPLCETMTTPKCHLSVLILSHCRLPDAVCRDLSEALKVAPALRELGLLQSRLTNTGLRLLCEGLA
  WPKCQVKTLRMQLPDLQEVINYLVIVLQQSPVLTTLDLSGCQLPGVIVEPLCAALKHPKCSLKTLSLTSV
  ELSENSLRDLQAVKTSKPDLSIIYSK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC031139
• ORF Size: 1338 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC031139, AAH31139
• RefSeq Size: 3454 bp
• RefSeq ORF: 1340 bp
• Locus ID: 101613
• MW: 126.6 kDa
• Gene Summary: The protein encoded by this gene binds arginine-vasopressin and may be involved in the arginine-vasopressin-mediated regulation of renal salt-water balance. The encoded protein also mediates inflammatory responses in the colon to allow recovery from intestinal epithelial damage and protects against tumorigenesis and the development of colitis. Finally, this protein can increase activation of NF-kappa-B, activation of CASP1 through interaction with ASC, and cAMP accumulation. [provided by RefSeq, Feb 2013]