Nlrp6 (BC031139) Mouse Tagged ORF Clone

CAT#: MR207117L4

  • LentiORF®

Lenti ORF clone of Nlrp6 (mGFP-tagged) - Mouse NLR family, pyrin domain containing 6 (cDNA clone MGC:37241 IMAGE:4972485)


  "BC031139" in other vectors (4)


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Reconstitution Protocol

USD 470.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag mGFP
Symbol Nlrp6
Synonyms AI504961|Avr|Nalp6|Navr|Navr; Avr|Non-AVR|Pypaf5
Vector pLenti-C-mGFP-P2A-Puro
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection Puromycin
Sequence Data
The ORF insert of this clone is exactly the same as(MR207117).
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN BC031139
ORF Size 1338 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq BC031139, AAH31139
RefSeq Size 3454 bp
RefSeq ORF 1340 bp
Locus ID 101613
Cytogenetics 7 F5
Gene Summary The protein encoded by this gene binds arginine-vasopressin and may be involved in the arginine-vasopressin-mediated regulation of renal salt-water balance. The encoded protein also mediates inflammatory responses in the colon to allow recovery from intestinal epithelial damage and protects against tumorigenesis and the development of colitis. Finally, this protein can increase activation of NF-kappa-B, activation of CASP1 through interaction with ASC, and cAMP accumulation. [provided by RefSeq, Feb 2013]

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