Mlkl (NM_029005) Mouse Tagged ORF Clone

CAT#: MR207406

Mlkl (Myc-DDK-tagged) - Mouse mixed lineage kinase domain-like (Mlkl)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Mlkl
• Synonyms: 9130019I15Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR207406 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATAAATTGGGACAGATCATCAAGTTAGGCCAGCTCATCTATGAACAGTGTGAAAAGATGAAATACT
  GCCGGAAACAATGCCAGCGTCTAGGAAACCGTGTGCACGGCCTGCTACAGCCTCTCCAGAGACTCCAGGC
  CCAAGGAAAGAAGAACCTGCCCGATGACATTACTGCTGCCCTGGGCCGTTTTGATGAAGTCCTGAAGGAG
  GCTAACCAGCAGATAGAAAAGTTCAGCAAGAAGTCCCATATTTGGAAGTTTGTGAGTGTGGGCAATGATA
  AGATCCTCTTCCATGAAGTGAATGAGAAGCTGAGAGACGTCTGGGAGGAGCTGTTGCTGCTGCTTCAGGT
  TTATCATTGGAATACCGTTTCAGATGTCAGCCAGCCAGCATCCTGGCAGCAGGAAGATCGACAGGATGCA
  GAGGAAGACGGAAATGAAAATATGAAAGTTATCCTGATGCAGTTGCAAATTAGCGTGGAAGAAATCAACA
  AAACCCTGAAGCAATGCTCACTAAAACCCACACAGGAGATCCCACAAGATCTCCAAATCAAGGAGATTCC
  AAAGGAACATCTTGGACCTCCGTGGACCAAACTGAAGACAAGTAAAATGAGCACCATTTATAGAGGAGAG
  TATCACAGATCTCCAGTTACCATCAAAGTATTCAACAACCCCCAGGCCGAAAGTGTTGGAATAGTGAGGT
  TCACTTTCAATGACGAGATCAAAACCATGAAGAAATTCGATTCTCCCAACATCTTGCGTATATTTGGGAT
  TTGCATTGATCAAACAGTGAAGCCCCCTGAGTTCTCCATTGTCATGGAGTACTGTGAACTTGGAACCCTG
  AGGGAACTGCTGGATAGAGAAAAAGACCTCACAATGAGTGTGCGCAGCCTCCTAGTCCTGAGGGCAGCCA
  GAGGCTTATACAGGCTACACCATTCGGAAACACTCCACAGAAACATCAGCAGCTCCAGTTTCCTCGTAGC
  CGGAGGCTACCAAGTAAAGCTTGCAGGATTTGAGTTAAGCAAAACACAGAATTCCATCAGCCGGACAGCA
  AAGAGCACTAAAGCAGAGAGATCCAGTTCAACGATATATGTCTCCCCTGAGAGACTGAAAAATCCATTTT
  GCCTTTATGACATAAAAGCTGAAATATATAGCTTTGGAATTGTACTCTGGGAAATTGCCACTGGAAAGAT
  CCCATTTGAAGGCTGTGATTCTAAGAAGATCCGGGAGCTGGTGGCTGAGGACAAGAAGCAGGAACCAGTG
  GGTCAGGATTGCCCTGAGTTGTTGCGGGAAATCATTAATGAGTGTCGTGCCCATGAGCCCTCCCAACGGC
  CCTCTGTGGACGGAATCTTGGAGAGACTGTCTGCGGTTGAAGAATCCACGGACAAGAAGGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR207406 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDKLGQIIKLGQLIYEQCEKMKYCRKQCQRLGNRVHGLLQPLQRLQAQGKKNLPDDITAALGRFDEVLKE
  ANQQIEKFSKKSHIWKFVSVGNDKILFHEVNEKLRDVWEELLLLLQVYHWNTVSDVSQPASWQQEDRQDA
  EEDGNENMKVILMQLQISVEEINKTLKQCSLKPTQEIPQDLQIKEIPKEHLGPPWTKLKTSKMSTIYRGE
  YHRSPVTIKVFNNPQAESVGIVRFTFNDEIKTMKKFDSPNILRIFGICIDQTVKPPEFSIVMEYCELGTL
  RELLDREKDLTMSVRSLLVLRAARGLYRLHHSETLHRNISSSSFLVAGGYQVKLAGFELSKTQNSISRTA
  KSTKAERSSSTIYVSPERLKNPFCLYDIKAEIYSFGIVLWEIATGKIPFEGCDSKKIRELVAEDKKQEPV
  GQDCPELLREIINECRAHEPSQRPSVDGILERLSAVEESTDKKV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_029005
• ORF Size: 1395 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_029005.1, NM_029005.2, NM_029005.3, NP_083281.1
• RefSeq Size: 1702 bp
• RefSeq ORF: 1395 bp
• Locus ID: 74568
• MW: 53.4 kDa
• Gene Summary: This gene belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain; however, is thought to lack protein kinase activity. This protein plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (Rip3), which is a key signaling molecule in necroptosis pathway. Knockout of this gene in mice showed that it is essential for necroptosis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2015]