Sfmbt1 (BC066188) Mouse Tagged ORF Clone

CAT#: MR207437

Sfmbt1 (Myc-DDK-tagged) - Mouse Scm-like with four mbt domains 1 (cDNA clone MGC:76655 IMAGE:30476111)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Sfmbt1
• Synonyms: Sfmbt, Smr
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR207437 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCGGGGAGCAGCAGCTTGATGCAGATCTTGGGTCTGGTGTAGAAGTAGAAGAATTCAGCTGGGAAG
  ATTATCTAGAAGAGACAGGCTCCACCACAGTTCCCTACGCATCGTTTAAACATGTGGATATACGTTTGCA
  AAATGGATTTGCTCCTGGGATGAAGCTGGAGGTAGCTCTGAAAAATGACCCTGAGACTTATTGGGTTGCC
  ACCATCATCACCGCCTGTGAGCAGTTGCTCCTGCTTCGTTATGAAGGCTATGGGGAAGACCGAAAAGCAG
  ACTTCTGGTGTGACATCAGGAAAGCTGGCCTCTACCCCATTGGGTGGTGTCAGCAGAACAAGAAGACCCT
  CGAAGCTCCAGAAGGCATCAGAGATAAAGTGTCTGATTGGAATGCATTCCTACAGCAGACCCTGATAGGA
  GCCTGTGGTCCTCCCGTGTCTCTGCTAGAGGGCCTCCGTAATGGAAGGAATCCTTTAGATCTCATTGCCC
  CAGGGTCCAAACTAGAATGCCAGGATTTTCGGGACTCTTTAAGCACTTGGCTTGTTACTGTTGTGGAGAA
  CATTGGGGGACGGTTGAAGCTGCGGTATGAAGGCCTTGAGAGCCGTGACGGTTTTGAACACTGGTTGTAT
  TACTTAGATCCATTTCTTCATCACATTGGTTGGGCTGCTCAGCAAGGATGTGACCTTCAGCCCCCATTAG
  CCATCAAACATCTGAAGAGTGAAGCTGACTGGCAAGAGATTTTGGCCAAAGTGAAAGAGGAAGAGCCATT
  ACCCTCTTACTTATTTAAGGATAAGCAAGTTATTGGAACCCATGAATTTTCTATAAACATGAAGTTGGAA
  GCTGTGGACCCGTGGTCTCCTTTTGGGATTTCTCCTGCAACGATTGCTAAGGTGTTTGATGATAAGTACT
  TTCTAGTGGAAATGGATGACCTTCGGCCAGAAGACCATACAAGGCGATCATTTGTGTGCCATGCTAACAG
  TCCTGGTATCTTTCCTGTGCAGTGGAGTCTAAAGAATGGTTTACATATCAATCCTCCTCCAGGCTTCCGG
  AGCCAGGACTTTGACTGGGCCGACTACCTCAAGCAGTGTGGGGCTGAAGCTGCTCCCCAGAAGTGCTTCC
  CGCAGTCAATTTCTGAGCACCAGTTTAAGGAGAACATGAAACTGGAGGCAGTGAACCCTCTTTTCCCTGA
  AGAAGTGTGCATTGCCACCGTTACTGCAGTGAGAGGCTCGTACCTGTGGCTCCAGCTAGAAGGTTCTAAG
  AAGCCTGTACCCGAATTTATTGTAAGTGCAGAGTCGATGAATATATTTCCTTTGGGCTGGTGTGAAACCA
  ATGGCCATCCTCTCAGTACTCCTCGCCGGGCACGAGGGCATAAACTGAGGAAAATCGCAGTGGTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR207437 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSGEQQLDADLGSGVEVEEFSWEDYLEETGSTTVPYASFKHVDIRLQNGFAPGMKLEVALKNDPETYWVA
  TIITACEQLLLLRYEGYGEDRKADFWCDIRKAGLYPIGWCQQNKKTLEAPEGIRDKVSDWNAFLQQTLIG
  ACGPPVSLLEGLRNGRNPLDLIAPGSKLECQDFRDSLSTWLVTVVENIGGRLKLRYEGLESRDGFEHWLY
  YLDPFLHHIGWAAQQGCDLQPPLAIKHLKSEADWQEILAKVKEEEPLPSYLFKDKQVIGTHEFSINMKLE
  AVDPWSPFGISPATIAKVFDDKYFLVEMDDLRPEDHTRRSFVCHANSPGIFPVQWSLKNGLHINPPPGFR
  SQDFDWADYLKQCGAEAAPQKCFPQSISEHQFKENMKLEAVNPLFPEEVCIATVTAVRGSYLWLQLEGSK
  KPVPEFIVSAESMNIFPLGWCETNGHPLSTPRRARGHKLRKIAVV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC066188
• ORF Size: 1395 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC066188, AAH66188
• RefSeq Size: 2111 bp
• RefSeq ORF: 1397 bp
• Locus ID: 54650
• MW: 52.7 kDa