Gpbp1 (BC067075) Mouse Tagged ORF Clone

CAT#: MR207554

Gpbp1 (Myc-DDK-tagged) - Mouse GC-rich promoter binding protein 1 (cDNA clone MGC:91361 IMAGE:30614988)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Gpbp1
• Synonyms: mGPBP, Gpbp
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR207554 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCAGCATGACTTTGCTCCAGCCTGGCTTAATTTTCCTACTCCTCCATCATCAACAAAGTCATCAT
  TGAATTTTGAGAAGCACTCTGAAAATTTTTCATGGACAGAAAATCGTTATGATGTGAGTCGTCGACGACA
  CAATTCTTCAGATGGCTTTGATTCTGGTATTGGACGTCCTAATGGAGGTAATTTTGGGAGGAAAGAGAAA
  AATGGATGGCGTACGCATGGCAGAAATGGTACAGAAAACATAAATCATCGTGGGGGATACCATGGTGGAA
  ATTCCCGTTCTCGTAGCAGTATTTTCCATTCTGGAAAAAGCCAAGGACTACATGAAAACAGCATCCCTGA
  CAATGAAACTGGGAGGAAAGAAGACAAAAGAGAACGCAGACAGTTTGAGGCTGAGGATTTTCCATCTTTA
  AATCCTGAATATGAGAGAGAACCAAATCAGAATAAATCTTTAGCTGCGGGTGTTTGGGACTATCCCCCGA
  ATCCTAAATCTAGAACTCCAAGAATGCTGGTCATTAAGAAAGGTAATACAAAAGACTTACAGCTATCTGG
  ATTCCCAGTAGCAGGAAACCTCCAGTCACAGCCAGTTAAGAATGGAACTGGTCCAAGTGTTTATAAAGGC
  TTAGTCCCCAAACCTGCTGTTCCACCTACAAAACCTACACAATGGAAAAGCCAAACTAAAGAAAACAAAG
  TCGGGACTTCTTTTTCTCATGAATCTACATATGGTGTTGGCAACTTTAATACTTTTAAGTCAACGGCCAA
  GAATATTAGTCCATCAACAAATTCAGTGAAAGAGTGTAATCGTTCAAATTCTTCTTCGCCTGTTGACAAA
  CTTAATCAGCAGCCTCGTTTAACTAAACTGACACGAATGCGCAGCGATAAAAAGAGTGAATTTTTGAAAG
  CATTGAAAAGGGACAGAGTGGAGGAGGAACATGAAGATGAAAGTCATGCTGGCTCAGAGAAGGACGACGA
  CTCATTTAATTTGCATAACAGCAATACTACTCACCAAGAAAGAGATATAAACAGAAACTTTGATGAAAAT
  GAAATTCCACAGGAGAACGGCAATGCCTCGATGATTTCTCAACAGATCATTCGTTCTTCGACTTTTCCAC
  AAACTGATGTTCTTTCCAGTTCACTAGAGGCAGAACACAGATTATTAAAAGAAATGGGCTGGCAGGAAGA
  CAGTGAAAATGATGAAACATGTGCTCCCTTAACTGAGGATGAAATGAGAGAATTCCAAGTTATTAGTGAA
  CAGTTACAGAAGAATGGTCTGAGAAAAAATGGTATTTTGAAAAATGGCCTGATCTGTGACTTCAAGTTTG
  GACCCTGGAAAAACAGCACTTTCAAACCCACAATTGAGAATGATGACACAGAGACAAGTAGCAGCGACAC
  GTCGGATGATGACGATGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR207554 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAQHDFAPAWLNFPTPPSSTKSSLNFEKHSENFSWTENRYDVSRRRHNSSDGFDSGIGRPNGGNFGRKEK
  NGWRTHGRNGTENINHRGGYHGGNSRSRSSIFHSGKSQGLHENSIPDNETGRKEDKRERRQFEAEDFPSL
  NPEYEREPNQNKSLAAGVWDYPPNPKSRTPRMLVIKKGNTKDLQLSGFPVAGNLQSQPVKNGTGPSVYKG
  LVPKPAVPPTKPTQWKSQTKENKVGTSFSHESTYGVGNFNTFKSTAKNISPSTNSVKECNRSNSSSPVDK
  LNQQPRLTKLTRMRSDKKSEFLKALKRDRVEEEHEDESHAGSEKDDDSFNLHNSNTTHQERDINRNFDEN
  EIPQENGNASMISQQIIRSSTFPQTDVLSSSLEAEHRLLKEMGWQEDSENDETCAPLTEDEMREFQVISE
  QLQKNGLRKNGILKNGLICDFKFGPWKNSTFKPTIENDDTETSSSDTSDDDDV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC067075
• ORF Size: 1419 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC067075.1
• RefSeq Size: 2880 bp
• RefSeq ORF: 1421 bp
• Locus ID: 73274
• MW: 53.4 kDa