Nfia (BC075702) Mouse Tagged ORF Clone

CAT#: MR207901

Nfia (Myc-DDK-tagged) - Mouse nuclear factor I/A (cDNA clone MGC:76704 IMAGE:30087137)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Nfia
• Synonyms: NF1-A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR207901 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTATTCTCCGCTCTGTCTCACCCAGGATGAGTTTCATCCTTTCATTGAAGCACTTCTGCCCCATGTCC
  GCGCCTTCGCCTACACATGGTTCAACCTGCAGGCCCGAAAGCGGAAATACTTCAAAAAACATGAGAAGCG
  CATGTCGAAAGAAGAGGAGAGGGCCGTGAAGGATGAACTGCTAAGCGAGAAGCCCGAGGTCAAGCAGAAG
  TGGGCTTCCCGACTTCTGGCCAAGTTACGGAAAGATATCCGACCCGAGTACCGAGAGGATTTTGTTCTTA
  CAGTTACAGGGAAAAAACCTCCATGCTGTGTTCTTTCCAACCCTGATCAGAAAGGCAAGATGCGGAGAAT
  TGACTGCCTCCGCCAGGCAGATAAAGTATGGAGGTTGGACCTCGTCATGGTGATCTTGTTCAAAGGTATT
  CCGCTGGAAAGTACTGATGGCGAGCGCCTTGTAAAGAGTCCACAGTGCTCTAATCCAGGGCTCTGTGTCC
  AGCCCCATCACATAGGGGTTTCTGTAAAGGAACTCGATTTATATTTGGCATACTTTGTACATGCAGCAGA
  TTCAAGTCAATCTGAAAGTCCCAGCCAGCCAAGTGAAGCTGACATTAAGGACCAGCCAGAAAATGGACAT
  TTGGGCTTCCAGGACAGCTTTGTCACATCAGGTGTTTTCAGTGTGACTGAGCTAGTAAGAGTGTCACAAA
  CACCAATAGCTGCAGGAACCGGCCCCAATTTTTCTCTCTCTGATTTGGAAAGTTCTTCATACTACAGCAT
  GAGTCCAGGAGCAATGAGGAGGTCTCTGCCCAGCACATCCTCTACCAGCTCTACAAAGCGCCTCAAGTCT
  GTGGAGGATGAAATGGACAGTCCTGGTGAAGAACCATTTTACACAGGCCAAGGGCGCTCCCCAGGGAGTG
  GCAGCCAGTCCAGTGGATGGCATGAAGTAGAGCCAGGCTTGCCATCTCCAAGCACACTGAAGAAGTCTGA
  GAAGTCTGGTTTCAGCAGCCCCTCCCCTTCGCAGACCTCCTCCCTGGGAACAGCATTCACACAGCATCAC
  CGACCTGTCATTACAGGACCCAGAGCAAGTCCACATGCGACGCCATCGACTCTCCACTTTCCAACGTCAC
  CCATCATCCAGCAGCCTGGGCCTTACTTCTCACACCCAGCCATCCGTTACCACCCTCAGGAGACGCTGAA
  AGAGTTTGTCCAACTTGTCTGTCCTGATGCTGGTCAGCAAGCTGGACAGGTGGGGTTCCTCAATCCCAAT
  GGAAGCAGTCAAGGCAAGGTGCACAACCCATTCCTCCCCACCCCAATGTTGCCGCCGCCGCCACCACCAC
  CGATGGCCAGACACCAAGCCTCCAACCACACCTCACCGACCTACTCGACACCCAGCACCTCCCCCGCAAA
  CCGATTCGTCAGTGTTGGACCACGGGATCCAAGCTTTGTAAATATCCCTCAACAGACACAGTCCTGGTAC
  CTGGGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR207901 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MYSPLCLTQDEFHPFIEALLPHVRAFAYTWFNLQARKRKYFKKHEKRMSKEEERAVKDELLSEKPEVKQK
  WASRLLAKLRKDIRPEYREDFVLTVTGKKPPCCVLSNPDQKGKMRRIDCLRQADKVWRLDLVMVILFKGI
  PLESTDGERLVKSPQCSNPGLCVQPHHIGVSVKELDLYLAYFVHAADSSQSESPSQPSEADIKDQPENGH
  LGFQDSFVTSGVFSVTELVRVSQTPIAAGTGPNFSLSDLESSSYYSMSPGAMRRSLPSTSSTSSTKRLKS
  VEDEMDSPGEEPFYTGQGRSPGSGSQSSGWHEVEPGLPSPSTLKKSEKSGFSSPSPSQTSSLGTAFTQHH
  RPVITGPRASPHATPSTLHFPTSPIIQQPGPYFSHPAIRYHPQETLKEFVQLVCPDAGQQAGQVGFLNPN
  GSSQGKVHNPFLPTPMLPPPPPPPMARHQASNHTSPTYSTPSTSPANRFVSVGPRDPSFVNIPQQTQSWY
  LG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC075702
• ORF Size: 1476 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC075702, AAH75702
• RefSeq Size: 3001
• RefSeq ORF: 1478
• Locus ID: 18027
• MW: 54.4 kDa
• Gene Summary: Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication. [UniProtKB/Swiss-Prot Function]