Srp54 (BC005543) Mouse Tagged ORF Clone

CAT#: MR208026

Srp54 (Myc-DDK-tagged) - Mouse signal recognition particle 54 (cDNA clone MGC:6231 IMAGE:3585216)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Srp54
• Synonyms: 54kDa
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR208026 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTTCTAGCAGATCTTGGAAGAAAAATAACATCAGCATTACGCTCATTGAGCAATGCCACCATTATCA
  ATGAAGAGGTATTAAATGCTATGCTGAAAGAAGTATGTACAGCATTATTGGAAGCAGATGTTAATATTAA
  ACTAGTGAAGCAACTCAGAGAAAATGTTAAGTCTGCAATTGATCTTGAAGAGATGGCATCTGGACTGAAC
  AAAAGAAAAATGATTCAGCATGCTGTATTTAAAGAACTTGTAAAGCTTGTAGACCCTGGAGTTAAAGCGT
  GGACACCGACTAAGGGAAAGCAAAATGTGATCATGTTTGTTGGATTGCAAGGGAGTGGTAAAACGACAAC
  ATGTTCAAAGTTAGCATATTATTACCAGAGGAAAGGTTGGAAGACCTGTTTGATATGTGCAGATACATTC
  AGAGCAGGAGCCTTTGACCAGCTAAAACAGAATGCCACCAAAGCAAGAATTCCGTTCTATGGCAGCTATA
  CTGAAATGGACCCTGTCATCATTGCTTCTGAAGGAGTGGAGAAATTCAAAAATGAAAATTTTGAAATTAT
  TATTGTTGATACAAGTGGTCGTCACAAACAAGAAGACTCTTTATTTGAAGAAATGCTTCAAGTTTCTAAT
  GCTATACAACCTGATAACATTGTTTATGTGATGGATGCATCCATCGGACAGGCTTGTGAGGCCCAGGCGA
  AGGCCTTTAAAGACAAAGTAGATGTAGCTTCAGTAATAGTGACAAAACTCGACGGTCACGCGAAAGGAGG
  CGGTGCTCTTAGTGCAGTTGCTGCCACAAAAAGTCCAATTATTTTCATTGGTACAGGGGAACATATAGAT
  AATTTTGAACCTTTCAAAACACAACCTTTCATCAGCAAACTCCTTGGAATGGGTGATATTGAAGGACTGA
  TTGATAAAGTCAATGAATTGAAGTTGGATGATAACAAGGCACTTATAGAGAAGTGGAAGCACGGTCAGTT
  TACATTGCGAGACATGTATGAACAGTTTCAGAATATTATGAAAATGGGCCCATTCAGTCAGATATTGGGG
  ATGATTCCTGGCTTTGGCACAGATTTTATGAGCAAAGGGAATGAGCAGGAGTCAATGGCAAGGCTGAAGA
  AACTGATGACAATCATGGACAGTATGAACGATCAAGAACTGGACAGTACAGATGGTGCCAAGGTTTTCAG
  TAAGCATCCAGGGAGAATCCAAAGAGTTGCCCGGGGATCAGGTGTGTCAACAAGAGATATTCAAGAACTT
  CTGACCCAGTATACCAAGTTTGCACAGATGGTCAAAAAGATGGGAGGTATCAAAGGACTTTTCAAAGGCG
  GTGATATGTCTAAGAATGTGAGTCAGTCACAGATGGCAAAATTAAACCAACAAATGGCCAAAATGATGGA
  CCCACGAGTTCTTCATCACATGGTGTGTGACTTTCTTGGAGAGAAGATTGCGTCAGTTTTGGGCATCAGC
  ACCCCAAAATACCAGTACGCCATTGATGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR208026 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MVLADLGRKITSALRSLSNATIINEEVLNAMLKEVCTALLEADVNIKLVKQLRENVKSAIDLEEMASGLN
  KRKMIQHAVFKELVKLVDPGVKAWTPTKGKQNVIMFVGLQGSGKTTTCSKLAYYYQRKGWKTCLICADTF
  RAGAFDQLKQNATKARIPFYGSYTEMDPVIIASEGVEKFKNENFEIIIVDTSGRHKQEDSLFEEMLQVSN
  AIQPDNIVYVMDASIGQACEAQAKAFKDKVDVASVIVTKLDGHAKGGGALSAVAATKSPIIFIGTGEHID
  NFEPFKTQPFISKLLGMGDIEGLIDKVNELKLDDNKALIEKWKHGQFTLRDMYEQFQNIMKMGPFSQILG
  MIPGFGTDFMSKGNEQESMARLKKLMTIMDSMNDQELDSTDGAKVFSKHPGRIQRVARGSGVSTRDIQEL
  LTQYTKFAQMVKKMGGIKGLFKGGDMSKNVSQSQMAKLNQQMAKMMDPRVLHHMVCDFLGEKIASVLGIS
  TPKYQYAIDE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC005543
• ORF Size: 1500 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: BC005543, AAH05543
• RefSeq Size: 1986
• RefSeq ORF: 1502
• Locus ID: 24067
• MW: 55.5 kDa