Parp8 (BC021315) Mouse Tagged ORF Clone

CAT#: MR208030

Parp8 (Myc-DDK-tagged) - Mouse poly (ADP-ribose) polymerase family, member 8 (cDNA clone MGC:29205 IMAGE:5027417)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Parp8
• Synonyms: 2810430O08Rik|ARTD16|D13Ertd275e
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR208030 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCACCATTAAATCGCACAAACTTTTGGGCCGGCCCTGCCCCTCAGCCGGCAAGCAAGAGGACTGCC
  TCACACTGAAGTCCCATAAGTTATTGACTCGATCTTGTTCTGGAGACCCACGATGTGAGCACAACACCAA
  CTTGAAGCCCCACAAACTGCTAAGCAGATCTTACTCCAGTAATCTCAGAATGGAAGAGTTATATGGCCTG
  AAAAATCACAAATTGCTCAGCAAGTCTTACTCCAGTGCCCCCAAGACATCGAAAATGGAGCATTTCAAGG
  AACCCAACGCAGAGGGCAGGAGGCTCTCGCTTACCTCAGGGCTTATCGGTATTTTAACACCATCATCGTC
  TTCTTCCCAGCCTCCTACAAATGGTGCAAAAAGCATTCCAATCCGAGACCGCGGCTTCCTGGTGCAGACC
  ATTGAGTTTGCTGAGCAGCGGATCCCTGTACTGAATGAATATTGTGTGGTGTGTGATGAACCACACGTGT
  TTCAAAATGGCCCCATGCTTAGGCCCACAGTATGTGAGCGAGAGCTGTGTGTGTTTGCTTTTCAAACCCT
  GGGAGTGATGAATGAAGCTGCCGACGAGATCGCTACCGGAGCTCAGGTGGTAGATCTGCTTGTGTCCATG
  TGTAGATCTGCGTTGGAATCTCCTAGAAAAGTTGTCATTTTTGAGCCATATCCTTCTGTGGTAGATCCTA
  ATGACCCTCAGATGTTAGCCTTCAATCCCAGGAAGAAGAACTATGATCGTGTAATGAAAGCTCTGGACAG
  CATAACTTCCATCAGAGAAATGACACAGGCACCCTATCTGGAAATCAAGAAGCAGATGGATAAACAGGAC
  CCCCTTGCTCATCCACTTCTGCAATGGGTCATCTCAAGTAATAGGTCACATATTGTGAAACTGCCAGTCA
  ACAGGCAACTGAAGTTCATGCACACGCCACACCAGTTCCTCCTCCTCAGCAGCCCACCAGCCAAAGAGTC
  CAACTTCAGAGCCGCCAAGAAGCTCTTCGGGAGCACCTTCGCATTTCATGGCTCTCACATTGAAAACTGG
  CACTCTATCCTGAGGAATGGTCTGGTCGTTGCCTCTAACACGCGTCTGCAGCTCCACGGTGCAATGTACG
  GAAGTGGGATCTATCTTAGCCCGATGTCCAGCATATCATTTGGTTACTCAGGGATGAACAAGAAGCAGAA
  GGTGTCAAGCAAGGACGAGCCAGCTTCCAGTAGTAAAAGCAGCAATGCATCACAGTCTCAGAAAAAAGGA
  CAGCAATCCCAGTTCCTGCAAAGCCGTAACTTGAAATGCATAGCCTTATGTGAAGTGATCACGTCACCTG
  ACCTGCACAAACATGGCGAGATATGGGTTGTCCCGAACACGGATCATGTCTGTACAAGATTCTTTTTTGT
  TTATGAAGATGGCCAGGTGGGAGATGCCAATATCAACACACAAGAAGGAGGTATTCACAAAGAGATACTC
  CGTGTGATTGGTAATCAGACTGCGACTGGT
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR208030 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSTIKSHKLLGRPCPSAGKQEDCLTLKSHKLLTRSCSGDPRCEHNTNLKPHKLLSRSYSSNLRMEELYGL
  KNHKLLSKSYSSAPKTSKMEHFKEPNAEGRRLSLTSGLIGILTPSSSSSQPPTNGAKSIPIRDRGFLVQT
  IEFAEQRIPVLNEYCVVCDEPHVFQNGPMLRPTVCERELCVFAFQTLGVMNEAADEIATGAQVVDLLVSM
  CRSALESPRKVVIFEPYPSVVDPNDPQMLAFNPRKKNYDRVMKALDSITSIREMTQAPYLEIKKQMDKQD
  PLAHPLLQWVISSNRSHIVKLPVNRQLKFMHTPHQFLLLSSPPAKESNFRAAKKLFGSTFAFHGSHIENW
  HSILRNGLVVASNTRLQLHGAMYGSGIYLSPMSSISFGYSGMNKKQKVSSKDEPASSSKSSNASQSQKKG
  QQSQFLQSRNLKCIALCEVITSPDLHKHGEIWVVPNTDHVCTRFFFVYEDGQVGDANINTQEGGIHKEIL
  RVIGNQTATG
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: BC021315
• ORF Size: 1500 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC021315, AAH21315
• RefSeq Size: 2143 bp
• RefSeq ORF: 1502 bp
• Locus ID: 52552
• MW: 55.7 kDa