Hyou1 (BC019785) Mouse Tagged ORF Clone

CAT#: MR208104

Hyou1 (Myc-DDK-tagged) - Mouse hypoxia up-regulated 1 (cDNA clone MGC:30561 IMAGE:5151731)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Hyou1
• Synonyms: Grp170, CBP-140
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR208104 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGCCACAGTAAGAAGGCAGAGGCCAAGGAGGCTACTCTGTTGGGCCTTGGTGGCTGTCCTCTTGG
  CAGACCTGTTGGCACTGAGCGACACATTGGCTGTGATGTCTGTAGACCTGGGCAGTGAATCCATGAAGGT
  GGCCATTGTCAAGCCTGGAGTGCCCATGGAGATTGTATTGAACAAGGAATCTCGGAGGAAAACTCCAGTG
  ACTGTGACCTTGAAAGAAAATGAAAGGTTTTTAGGTGATAGTGCAGCCGGCATGGCCATCAAGAACCCAA
  AGGCTACGCTCCGTTATTTCCAGCACCTCCTTGGAAAACAGGCGGATAACCCTCATGTGGCCCTTTACCG
  GTCCCGTTTCCCAGAACATGAGCTAATTGTTGACCCACAGAGGCAGACTGTGCGCTTCCAGATCAGTCCG
  CAGCTGCAGTTCTCTCCCGAGGAGGTACTGGGCATGGTTCTGAACTACTCCCGTTCCTTGGCTGAAGATT
  TTGCTGAACAACCCATTAAGGATGCAGTGATCACCGTGCCAGCCTTTTTCAACCAGGCTGAGCGCCGAGC
  TGTGCTGCAGGCTGCTCGGATGGCTGGCCTCAAGGTGCTGCAGCTCATCAATGACAACACTGCCACAGCC
  CTCAGCTACGGTGTCTTCCGCCGGAAAGATATCAATTCCACTGCACAGAACGTCATGTTCTATGACATGG
  GCTCGGGCAGCACTGTGTGCACCATCGTCACCTACCAGACAGTGAAGACTAAGGAGGCTGGGATGCAACC
  ACAGCTGCAGATCCGGGGCGTGGGATTTGACCGCACCCTGGGTGGCCTGGAGATGGAGCTTCGGCTTCGA
  GAACACCTGGCTAAGCTCTTCAATGAGCAGCGCAAGGGCCAGAAAGCCAAGGATGTTCGGGAAAACCCCC
  GGGCCATGGCCAAACTGCTTCGGGAAGCCAACCGGCTTAAAACCGTCCTGAGTGCCAACGCTGATCACAT
  GGCACAGATTGAGGGCTTGATGGATGATGTGGACTTCAAGGCCAAAGTAACTCGGGTGGAATTCGAGGAG
  CTGTGTGCAGATTTGTTTGACCGTGTGCCTGGACCTGTGCAGCAGGCCTTGCAGAGTGCAGAGATGAGCT
  TGGATCAAATTGAGCAGGTGATCCTGGTGGGCGGGGCCACTCGTGTTCCCAAAGTTCAAGAAGTGCTGCT
  CAAGGCCGTGGGCAAGGAGGAACTAGGAAAGAACATCAATGCGGACGAAGCTGCTGCCATGGGGGCTGTG
  TACCAGGCAGCGGCGCTCAGCAAGGCCTTCAAAGTGAAGCCATTTGTTGTGCGGGATGCTGTCATTTACC
  CAATCCTGGTGGAGTTCACAAGGGAGGTGGAGGAGGAGCCTGGGCTCCGAAGCCTGAAACACAATAAGCG
  TGTGCTCTTCTCCCGAATGGGGCCCTACCCTCAGCGCAAAGTCATCACCTTTAACCGCTACAGCCATGAT
  TTCAACTTCCACATCAACTACGGGTATTTGGCTCCCAGAATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR208104 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAATVRRQRPRRLLCWALVAVLLADLLALSDTLAVMSVDLGSESMKVAIVKPGVPMEIVLNKESRRKTPV
  TVTLKENERFLGDSAAGMAIKNPKATLRYFQHLLGKQADNPHVALYRSRFPEHELIVDPQRQTVRFQISP
  QLQFSPEEVLGMVLNYSRSLAEDFAEQPIKDAVITVPAFFNQAERRAVLQAARMAGLKVLQLINDNTATA
  LSYGVFRRKDINSTAQNVMFYDMGSGSTVCTIVTYQTVKTKEAGMQPQLQIRGVGFDRTLGGLEMELRLR
  EHLAKLFNEQRKGQKAKDVRENPRAMAKLLREANRLKTVLSANADHMAQIEGLMDDVDFKAKVTRVEFEE
  LCADLFDRVPGPVQQALQSAEMSLDQIEQVILVGGATRVPKVQEVLLKAVGKEELGKNINADEAAAMGAV
  YQAAALSKAFKVKPFVVRDAVIYPILVEFTREVEEEPGLRSLKHNKRVLFSRMGPYPQRKVITFNRYSHD
  FNFHINYGYLAPRI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC019785
• ORF Size: 1512 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC019785, AAH19785
• RefSeq Size: 2027 bp
• RefSeq ORF: 1514 bp
• Locus ID: 12282
• MW: 56.6 kDa