Cybb (NM_007807) Mouse Tagged ORF Clone

CAT#: MR208975

Cybb (Myc-DDK-tagged) - Mouse cytochrome b-245, beta polypeptide (Cybb)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cybb
• Synonyms: C88302; Cgd; CGD91-phox; Cyd; gp91-1; gp91phox; Nox2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR208975 representing NM_007807
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGAACTGGGCTGTGAATGAAGGACTCTCCATCTTTGTCATTCTGGTGTGGTTGGGGCTGAATGTCT
  TCCTCTTTATCAACTACTACAAGGTTTATGATGATGGGCCTAAGTATAATTATACTCGAAAACTCCTTGG
  GTCAGCACTGGCTCTGGCCCGGGCACCTGCAGCCTGCCTGAATTTCAACTGTATGCTGATCCTGCTGCCA
  GTGTGTCGAAATCTGCTCTCCTTTCTCAGGGGTTCCAGTGCGTGTTGCTCGACAAGGATTCGAAGACAAC
  TGGACAGGAACCTCACTTTCCATAAGATGGTAGCTTGGATGATAGCACTGCACACCGCCATCCACACAAT
  TGCACATCTCTTCAACGTTGAGTGGTGTGTGAATGCCAGAGTCGGGATTTCTGACCGGTATTCAATAGCT
  CTCTCTGACATCGGTGACAATGAGAACGAAGAGTATCTCAATTTTGCTAGAGAAAAAATCAAGAACCCTG
  AAGGGGGCCTGTATGTGGCCGTGACTCGGTTGGCTGGGATCACAGGAATTGTCATCACACTGTGTCTCAT
  ATTGATTATCACATCCTCTACCAAAACCATTCGGAGGTCTTATTTTGAAGTGTTCTGGTATACACACCAT
  CTCTTTGTGATCTTCTTCATCGGCCTTGCCATCCATGGAGCTGAACGAATTGTACGTGGACAGACTGCGG
  AGAGTTTGGAAGAGCATAATTTAGATATCTGTGCAGACAAAATCGAAGAATGGGGAAAAATAAAGGAGTG
  CCCAGTACCAAAGTTCGCTGGAAACCCTCCTATGACTTGGAAATGGATAGTGGGTCCTATGTTCCTGTAC
  CTTTGTGAGAGGTTGGTTCGGTTTTGGCGATCTCAGCAAAAGGTGGTCATCACCAAGGTGGTCACCCACC
  CTTTCAAAACCATTGAGCTTCAGATGAAGAAGAAGGGATTCAAGATGGAGGTGGGACAGTACATTTTTGT
  CAAGTGCCCCAAGGTATCCAAGTTAGAATGGCACCCATTCACACTGACCTCTGCTCCTGAGGAAGACTTT
  TTTAGCATCCATATCCGTATTGTGGGAGACTGGACGGAGGGGCTATTCAATGCTTGTGGCTGTGATAAGC
  AGGAGTTCCAAGATGCCTGGAAACTACCTAAGATAGCAGTTGATGGACCCTTTGGTACAGCCAGTGAAGA
  TGTGTTCAGCTATGAGGTGGTGATGTTAGTGGGAGCCGGGATTGGAGTCACGCCCTTTGCCTCCATTCTC
  AAGTCTGTCTGGTACAAATATTGTGATAATGCCACCAGTCTGAAACTCAAAAAGATCTACTTCTACTGGC
  TGTGCAGGGACACGCATGCCTTTGAGTGGTTTGCAGACCTGCTGCAGCTGCTGGAGACCCAGATGCAGGA
  AAGGAACAATGCCAACTTCCTCAGCTACAATATCTACCTTACTGGCTGGGATGAATCTCAGGCCAATCAC
  TTTGCTGTGCACCATGATGAGGAGAAAGATGTGATCACAGGCCTAAAACAGAAGACTCTGTATGGACGGC
  CCAACTGGGATAACGAGTTCAAGACCATTGCAAGTGAACACCCTAACACCACAATAGGCGTTTTCCTGTG
  TGGCCCTGAAGCCTTGGCTGAAACTCTCAGTAAACAGAGCATCTCCAACTCAGAATCCGGCCCGCGTGGA
  GTCCACTTCATTTTCAACAAGGAAAACTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR208975 representing NM_007807
  Red=Cloning site Green=Tags(s)
  
  MGNWAVNEGLSIFVILVWLGLNVFLFINYYKVYDDGPKYNYTRKLLGSALALARAPAACLNFNCMLILLP
  VCRNLLSFLRGSSACCSTRIRRQLDRNLTFHKMVAWMIALHTAIHTIAHLFNVEWCVNARVGISDRYSIA
  LSDIGDNENEEYLNFAREKIKNPEGGLYVAVTRLAGITGIVITLCLILIITSSTKTIRRSYFEVFWYTHH
  LFVIFFIGLAIHGAERIVRGQTAESLEEHNLDICADKIEEWGKIKECPVPKFAGNPPMTWKWIVGPMFLY
  LCERLVRFWRSQQKVVITKVVTHPFKTIELQMKKKGFKMEVGQYIFVKCPKVSKLEWHPFTLTSAPEEDF
  FSIHIRIVGDWTEGLFNACGCDKQEFQDAWKLPKIAVDGPFGTASEDVFSYEVVMLVGAGIGVTPFASIL
  KSVWYKYCDNATSLKLKKIYFYWLCRDTHAFEWFADLLQLLETQMQERNNANFLSYNIYLTGWDESQANH
  FAVHHDEEKDVITGLKQKTLYGRPNWDNEFKTIASEHPNTTIGVFLCGPEALAETLSKQSISNSESGPRG
  VHFIFNKENF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007807
• ORF Size: 1710 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_007807.1, NM_007807.2, NM_007807.3, NM_007807.4, NM_007807.5, NP_031833.3
• RefSeq Size: 4750
• RefSeq ORF: 1713
• Locus ID: 13058
• MW: 65.8 kDa
• Gene Summary: This gene encodes the heavy chain component of a heterodimeric transmembrane ion transporter composed of both a heavy and a light chain. This transporter mediates the transfer of electrons from nicotinamide adenine dinucleotide phosphate (NADPH) to oxygen to generate superoxide. This reaction is important in the innate immune response to pathogens. However, increased activity of the encoded protein also leads to the generation of reactive oxygen species that result in oxidative stress and can cause tissue damage. Conversely, loss of function of the related gene in human causes chronic granulomatous disease. Alternative splicing results in multiple transcript variants, although the full-length nature of some of these variants has not been determined. [provided by RefSeq, May 2013]