Dll3 (NM_007866) Mouse Tagged ORF Clone

CAT#: MR209139

Dll3 (Myc-DDK-tagged) - Mouse delta-like 3 (Drosophila) (Dll3)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Dll3
• Synonyms: pu; pudgy
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR209139 representing NM_007866.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGTCTCTCTGCAGGTGTCTCCGCTTTCCCAGACGCTGATCCTGGCTTTTCTTCTTCCTCAGGCACTG
  CCAGCTGGTGTCTTCGAGCTACAAATTCATTCTTTCGGGCCAGGCCCAGGCCTCGGGACCCCACGCTCC
  CCCTGCAACGCCCGAGGCCCTTGCCGCCTCTTCTTCAGGGTCTGCCTGAAGCCCGGAGTCTCCCAGGAG
  GCCACCGAGTCCCTGTGCGCCCTGGGCGCAGCACTGAGCACGAGCGTCCCGGTCTATACGGAGCACCCC
  GGAGAGTCAGCGGCTGCCCTGCCGCTGCCTGATGGCCTCGTACGTGTGCCCTTCCGCGATGCTTGGCCG
  GGCACCTTCTCCCTCGTCATTGAAACCTGGAGAGAGCAGCTGGGAGAGCATGCTGGAGGGCCCGCCTGG
  AACCTGCTAGCACGTGTGGTCGGCCGTAGACGCCTGGCGGCTGGGGGCCCGTGGGCCCGCGATGTGCAG
  CGCACAGGCACATGGGAGTTGCACTTCTCCTACCGCGCGCGGTGCGAGCCGCCCGCCGTCGGGGCCGCC
  TGCGCGCGCCTGTGCCGCTCACGCAGTGCCCCCTCGCGGTGTGGCCCGGGACTGCGACCCTGCACGCCA
  TTCCCAGACGAGTGCGAAGCCCCGTCTGTGTGTCGACCAGGCTGCAGCCCCGAGCACGGCTACTGTGAA
  GAGCCTGATGAATGCCGTTGCCTGGAGGGCTGGACTGGACCCCTCTGCACGGTCCCTGTCTCCACCAGT
  AGCTGCCTGAACTCCAGGGTTCCTGGTCCTGCCAGCACTGGATGCCTTTTACCTGGGCCTGGACCTTGT
  GATGGGAACCCATGTGCCAATGGGGGCAGCTGTAGTGAAACCTCTGGCTCCTTTGAATGTGCCTGTCCC
  CGGGGATTCTACGGGCTTCGATGTGAGGTGAGCGGGGTCACGTGCGCAGATGGACCCTGCTTCAATGGC
  GGCTTGTGTGTTGGCGGTGAAGATCCTGACTCTGCCTATGTCTGTCATTGCCCACCTGGTTTCCAAGGC
  TCTAACTGTGAGAAGAGGGTGGACCGCTGTAGCCTGCAGCCATGTCAGAATGGCGGCCTCTGCCTGGAC
  CTGGGCCACGCGTTGCGCTGCCGCTGTCGCGCGGGATTCGCCGGGCCGCGCTGCGAGCACGACCTGGAC
  GACTGCGCCGGCCGCGCCTGTGCCAACGGCGGCACGTGCGTGGAGGGCGGCGGCTCGCGCCGCTGCTCC
  TGTGCGCTGGGCTTCGGCGGGCGCGACTGCCGAGAACGCGCCGACCCCTGCGCCTCCCGCCCCTGCGCG
  CATGGAGGCCGTTGCTACGCCCACTTCTCTGGCCTGGTCTGCGCCTGCGCGCCCGGCTACATGGGCGTG
  AGATGCGAGTTCGCTGTGCGCCCGGACGGCGCGGACGCGGTGCCCGCCGCCCCGCGGGGCCTGAGGCAG
  GCGGATCCACAGCGCTTTCTTCTGCCTCCCGCCTTGGGGCTGCTGGTGGCCGCCGGTTTGGCTGGCGCC
  GCACTCTTGGTCATCCACGTTCGCCGCCGAGGTCCTGGCCAGGATACCGGGACTCGCCTGCTTTCTGGG
  ACCCGGGAGCCTTCGGTCCACACGCTCCCGGATGCACTCAACAACCTGAGGTTACAAGACGGTGCTGGG
  GATGGCCCCAGTTCGTCGGCTGACTGGAATCATCCTGAAGATGGAGACTCTAGATCCATTTATGTCATA
  CCAGCCCCTTCCATTTATGCACGAGAGGCC
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGAT
  ATCCTGGATTACAAGGATGACGACGATAAGGTTTAA
  
  Protein Sequence

  >Peptide sequence encoded by MR209139
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MVSLQVSPLSQTLILAFLLPQALPAGVFELQIHSFGPGPGLGTPRSPCNARGPCRLFFRVCLKPGVSQE
  ATESLCALGAALSTSVPVYTEHPGESAAALPLPDGLVRVPFRDAWPGTFSLVIETWREQLGEHAGGPAW
  NLLARVVGRRRLAAGGPWARDVQRTGTWELHFSYRARCEPPAVGAACARLCRSRSAPSRCGPGLRPCTP
  FPDECEAPSVCRPGCSPEHGYCEEPDECRCLEGWTGPLCTVPVSTSSCLNSRVPGPASTGCLLPGPGPC
  DGNPCANGGSCSETSGSFECACPRGFYGLRCEVSGVTCADGPCFNGGLCVGGEDPDSAYVCHCPPGFQG
  SNCEKRVDRCSLQPCQNGGLCLDLGHALRCRCRAGFAGPRCEHDLDDCAGRACANGGTCVEGGGSRRCS
  CALGFGGRDCRERADPCASRPCAHGGRCYAHFSGLVCACAPGYMGVRCEFAVRPDGADAVPAAPRGLRQ
  ADPQRFLLPPALGLLVAAGLAGAALLVIHVRRRGPGQDTGTRLLSGTREPSVHTLPDALNNLRLQDGAG
  DGPSSSADWNHPEDGDSRSIYVIPAPSIYAREA
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
  
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007866
• ORF Size: 1755 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007866.2, NP_031892.2
• RefSeq Size: 2163 bp
• RefSeq ORF: 1758 bp
• Locus ID: 13389
• UniProt ID: O88516
• Cytogenetics: 7 16.67 cM
• MW: 61.1 kDa
• Gene Summary: Inhibits primary neurogenesis. May be required to divert neurons along a specific differentiation pathway. Plays a role in the formation of somite boundaries during segmentation of the paraxial mesoderm.[UniProtKB/Swiss-Prot Function]