Ncoa4 (BC031528) Mouse Tagged ORF Clone

CAT#: MR209447

Ncoa4 (Myc-DDK-tagged) - Mouse nuclear receptor coactivator 4 (cDNA clone MGC:28270 IMAGE:4008986)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ncoa4
• Synonyms: Rfg
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR209447 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACACATCCCTGGAACAGAGTGGCTGCTACAGTAATAGAGAAACTCTTCTGAGGTGCAGTGATGCAA
  GGAGGGAGCTGGAGCTTGCTATTGGTGGAGTACTTCGGGCTGAACAGCAAATTAAAGATAATTTACGAGA
  GGTAAAAGCTCAGATTCACAGCTGCATAAGTCGCCACCTGGAATGCCTTCGGAGCCGAGAGGTGTGGCTC
  AATGAACAGGTCGATCTCATCTATCAGCTTAAAGAGGAGACACTGCAGCAGCAGGCTCAGCAGCTCTATT
  GGCTAATGGGCCAATTCAATTGTCTTATTCATCAACTGGAGTATACTCAGAACAAAGATCTTGCCAATCA
  AGTCTCTGTGTGCTTGGAGAGACTGGGCAGTTTGGCCCTGAAGCCTGAAGATTCAACTGTCCTACTCTTT
  GAAGCAGATACATCTGCTCTGCGCCAGACCATCACCACATTTGGATCCCTTAAGACCATTCAAATCCCTG
  AGCATCTGATGGCTCACGCGAGCTCCTCAAGTATTGGGCCTTTCCTAGAGAAAAGAGGCTATATCCAGGT
  GCCAGAGCAGAAGTCAGCATCCAGTGGCACAGCTGTATCTCTGAGTGAGTGGCTTCTTGTAAGCAAACCT
  GCCATTGGTCTTCAGGCTCCTTATGTACCCAGTACCAACCCACAGGACTGGCTTATCCCAAAACAGACTT
  CAGAGAATAGTCAGACTTCTGCCAGAGCCTGCAGTTTCTTCAGCGATGCCTGGGGCAATCTGAAGGGCTT
  AGAAAATTGGCTCCTTAACAGTCATCAACAAGAAATTGCTGGAAAACCAAGTTCCTCAAAATGTAACAGC
  CATTGTAGCACCAGTTCTTTCTCTCCTGAAGCTGAAAAGGCTGAAGATGTAGAGCTCCTTGATCAAGACG
  AGCTGGACCTGTCTGATTGGTTGGTGACTCCTCAGGAACCCTGTGAGCTGGAGAAGCCTGTGGATGGCAG
  CTGGGAAACCAGCGAGAAGTTTAAACTCTTGTTCCAGGTGTTCAGAGAGCCCTACAATGTGAGTGATTGG
  CTTGTCAAGCCTGACTCCTGTACCAACTGTCAGGGCAACCAGCCTAGAGGTGTGGAGATTGAGAACCTGG
  GCAATCTGAAATGCCTAAATGACCACTTGGAGGCCAAAAAATCAGTGTCGGTCCCCAGCGCAACAATCAG
  CGAGGGCTGGCTTGCCCAGAACCATCAGGACACATGGAAAGTAGAGGAAGTGTGTAAAGCCAACGAGCCT
  TGCACCAGTTTTGCAGAGTGTGTGTGTGATGACAACTGTGAGAAGGAAGCCGTGTATAAATGGCTTCTGA
  AGAAGGGAGGAAAGGACAAGAATGGAATGCCTATGGAACCTAAATCTGAGCCTGAGAAACACAGAGAGTC
  CCTGACACTGTGGCTCTGCCCTTCCAGAAATGAGCTAACAGAACAAGCTAAGGCACCCAAGGCCTTGGCT
  CCTGCTAGAATTGCTGATTCCTTCCACGTCATAAAGAATAGTTCCTTGTCAGAGTGGCTTATAGGGCCTA
  CGTGCAAAGGAGGCCCTAAGGACGTGCCTAATACAGAGGAGAGAGCTGGCAAAGAGATGCTTCAAAGCTC
  CATGGCCACTTCCTGGTGTCCCTTTAACACTGCCGACTGGGTTTTACCAGGAAAGAAAGTGGGAAGCCTC
  AGCCAGTTCCCCTCTGGAGAAGATAAGTGGCTACTTCGAAAGAAGGCACAGGAAGAACGTAACTTTCGCC
  CCGACTGTTACGGCCTCCCAGCAGTTTGTGATCTCTTTGCCTGTATGCAGCTTAAGGTTGATAAAGAAAA
  ATGGCTGTACAGAACTCCTCTACAGATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR209447 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MNTSLEQSGCYSNRETLLRCSDARRELELAIGGVLRAEQQIKDNLREVKAQIHSCISRHLECLRSREVWL
  NEQVDLIYQLKEETLQQQAQQLYWLMGQFNCLIHQLEYTQNKDLANQVSVCLERLGSLALKPEDSTVLLF
  EADTSALRQTITTFGSLKTIQIPEHLMAHASSSSIGPFLEKRGYIQVPEQKSASSGTAVSLSEWLLVSKP
  AIGLQAPYVPSTNPQDWLIPKQTSENSQTSARACSFFSDAWGNLKGLENWLLNSHQQEIAGKPSSSKCNS
  HCSTSSFSPEAEKAEDVELLDQDELDLSDWLVTPQEPCELEKPVDGSWETSEKFKLLFQVFREPYNVSDW
  LVKPDSCTNCQGNQPRGVEIENLGNLKCLNDHLEAKKSVSVPSATISEGWLAQNHQDTWKVEEVCKANEP
  CTSFAECVCDDNCEKEAVYKWLLKKGGKDKNGMPMEPKSEPEKHRESLTLWLCPSRNELTEQAKAPKALA
  PARIADSFHVIKNSSLSEWLIGPTCKGGPKDVPNTEERAGKEMLQSSMATSWCPFNTADWVLPGKKVGSL
  SQFPSGEDKWLLRKKAQEERNFRPDCYGLPAVCDLFACMQLKVDKEKWLYRTPLQM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC031528
• ORF Size: 1848 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC031528, AAH31528
• RefSeq Size: 3378 bp
• RefSeq ORF: 1850 bp
• Locus ID: 27057
• MW: 69.4 kDa