Acadvl (NM_017366) Mouse Tagged ORF Clone

CAT#: MR209811

Acadvl (Myc-DDK-tagged) - Mouse acyl-Coenzyme A dehydrogenase, very long chain (Acadvl), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Acadvl
• Synonyms: vlcad
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR209811 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGTCGGCTCGGATGACCCCGAGTGTGGGGCGACAGCTGCTGCGGCTGGGGGCCCGAAGCTCGCGAT
  CTACTACTGTGCTTCAGGGACAACCCCGGCCTATCTCTGCCCAGCGACTTTATGCCAGGGAGGCCACTCA
  GGCAGTTCTGGACAAGCCAGAAACCCTCTCCTCTGATGCTTCCACCAGAGAAAAACCAGCCAGGGCAGAA
  TCGAAGTCCTTTGCTGTGGGGATGTTCAAAGGCCAGCTTACCATTGATCAGGTGTTCCCATACCCATCTG
  TGCTCAGTGAAGAACAGGCACAATTTCTCAAAGAGCTGGTGGGACCAGTGGCCCGGTTCTTTGAGGAAGT
  GAATGACCCTGCCAAGAACGACGCCTTGGAGAAGGTGGAGGACGACACTTTGCAGGGACTCAAGGAACTG
  GGGGCATTTGGCCTGCAAGTACCCAGCGAGCTGGGTGGTTTGGGCCTCTCTAATACCCAGTATGCTCGCT
  TGGCAGAGATTGTGGGCATGCATGACCTTGGTGTTAGCGTTACCCTGGGAGCTCATCAGAGCATCGGTTT
  CAAAGGCATCTTGCTCTATGGCACAAAGGCCCAGAGAGAAAAATACCTTCCCAGAGTGGCATCTGGGCAG
  GCTTTGGCTGCTTTCTGCCTAACAGAGCCCTCGAGTGGATCCGATGTAGCCTCCATCCGAAGCTCAGCCA
  TACCCAGCCCCTGTGGAAAATATTACACTCTCAATGGGAGCAAGATCTGGATCAGTAATGGGGGCCTGGC
  CGACATTTTCACTGTCTTTGCCAAGACGCCAATTAAAGATGCAGCCACGGGGGCCGTGAAAGAGAAGATC
  ACAGCTTTTGTAGTGGAAAGGAGCTTCGGAGGGGTTACCCATGGGCTCCCTGAAAAGAAGATGGGCATCA
  AAGCATCTAACACGTCAGAGGTGTACTTTGATGGAGTGAAGGTGCCATCAGAGAATGTGCTAGGAGAGGT
  GGGAGATGGCTTCAAGGTTGCTGTCAACATCCTCAACAACGGAAGATTTGGGATGGCTGCAACCCTCGCA
  GGCACCATGAAATCCCTCATTGCCAAGGCGGTTGATCATGCTACTAATCGTACCCAGTTTGGGGACAAAA
  TTCACAACTTTGGGGTGATCCAGGAAAAGCTGGCTCGGATGGCTATTCTGCAGTATGTGACTGAGTCCAT
  GGCTTACATGCTGAGTGCCAACATGGACCAGGGATTCAAAGACTTCCAGATAGAAGCCGCCATCAGCAAA
  ATCTTTTGCTCGGAGGCGGCCTGGAAAGTGGCAGATGAGTGCATCCAAATAATGGGGGGCATGGGCTTCA
  TGAAGGAACCAGGGGTGGAGCGTGTGCTCCGAGATATTCGAATCTTCCGGATCTTTGAAGGGGCAAATGA
  CATTCTTCGACTGTTTGTGGCTCTCCAAGGCTGTATGGACAAAGGAAAGGAACTCACTGGGCTGGGCAAT
  GCCCTGAAGAATCCTTTTGGAAACGTTGGCCTCCTGATGGGAGAAGCAGGCAAACAGCTGAGACGGAGGA
  CAGGAATCGGCAGTGGTTTGAGTCTGTCAGGGATTGTCCACCCAGAGTTAAGTCGCAGTGGTGAACTGGC
  AGTGCAGGCTCTGGATCAATTTGCCACCGTGGTGGAGGCCAAGCTGGTGAAACACAAGAAAGGGATTGTC
  AACGAGCAGTTCCTGCTGCAGCGACTGGCGGACGGCGCCATTGACCTCTATGCCATGGTGGTGGTTCTGT
  CCAGAGCCTCAAGGTCCCTGAGTGAGGGCTACCCGACGGCACAGCATGAGAAAATGCTCTGTGATAGCTG
  GTGCATTGAGGCTGCAACTCGGATCCGAGAAAACATGGCCAGTCTGCAGTCCAGCCCTCAGCATCAGGAG
  CTCTTCCGGAACTTCAGAAGCATCTCCAAGGCCATGGTGGAGAATGGTGGCCTGGTCACCGGTAACCCCC
  TGGGAATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR209811 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MQSARMTPSVGRQLLRLGARSSRSTTVLQGQPRPISAQRLYAREATQAVLDKPETLSSDASTREKPARAE
  SKSFAVGMFKGQLTIDQVFPYPSVLSEEQAQFLKELVGPVARFFEEVNDPAKNDALEKVEDDTLQGLKEL
  GAFGLQVPSELGGLGLSNTQYARLAEIVGMHDLGVSVTLGAHQSIGFKGILLYGTKAQREKYLPRVASGQ
  ALAAFCLTEPSSGSDVASIRSSAIPSPCGKYYTLNGSKIWISNGGLADIFTVFAKTPIKDAATGAVKEKI
  TAFVVERSFGGVTHGLPEKKMGIKASNTSEVYFDGVKVPSENVLGEVGDGFKVAVNILNNGRFGMAATLA
  GTMKSLIAKAVDHATNRTQFGDKIHNFGVIQEKLARMAILQYVTESMAYMLSANMDQGFKDFQIEAAISK
  IFCSEAAWKVADECIQIMGGMGFMKEPGVERVLRDIRIFRIFEGANDILRLFVALQGCMDKGKELTGLGN
  ALKNPFGNVGLLMGEAGKQLRRRTGIGSGLSLSGIVHPELSRSGELAVQALDQFATVVEAKLVKHKKGIV
  NEQFLLQRLADGAIDLYAMVVVLSRASRSLSEGYPTAQHEKMLCDSWCIEAATRIRENMASLQSSPQHQE
  LFRNFRSISKAMVENGGLVTGNPLGI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017366
• ORF Size: 1971 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017366.1, NM_017366.2, NM_017366.3, NP_059062.1
• RefSeq Size: 2205 bp
• RefSeq ORF: 1971 bp
• Locus ID: 11370
• MW: 70.9 kDa
• Gene Summary: This gene encodes a homodimeric mitochondrial flavoprotein and is a member of the acyl-CoA dehydrogenase family. Members of this family catalyze the first step of fatty acid beta-oxidation, forming a C2-C3 trans-double bond in a FAD-dependent reaction. As beta-oxidation cycles through its four steps, each member of the acyl-CoA dehydrogenase family works at an optimum fatty acid chain-length. This enzyme has its optimum length between C16- and C20-acylCoA and localizes to the inner mitochondrial membrane (unlike related acyl-CoA dehydrogenases). In mice, deficiency of this gene can cause ventricular arrhythmias as well as fasting and cold intolerance. [provided by RefSeq, Nov 2012]