Bcl11b (NM_021399) Mouse Tagged ORF Clone

CAT#: MR210782

Bcl11b (Myc-DDK-tagged) - Mouse B-cell leukemia/lymphoma 11B (Bcl11b), transcript variant 2

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Bcl11b
• Synonyms: 9130430L19Rik; AI604821; B630002E05Rik; BCL-11B; Ctip2; Rit1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210782 representing NM_021399.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGTCCCGCCGCAAACAGGGCAACCCGCAGCACTTGTCCCAGAGGGAACTCATCACGCCAGAGGCTGAC
  CATGTGGAGGCTACCATCCTCGAGGAAGACGAGGGTCTGGAGATAGAGGAGCCTAGCAGCCTGGGGCTG
  ATGGTGGGAGGCCCCGACCCTGATCTACTCACCTGTGGCCAGTGTCAGATGAACTTCCCGCTGGGGGAC
  ATCCTGGTTTTTATAGAGCACAAGAAGAAACAGTGTGGAGGCCTGGGCCCCTGCTACGACAAGGTCCTG
  GACAAGAGCAGTCCACCTCCCTCCTCTCGCTCTGAGCTCAGGAGAGTATCTGAGCCAGTGGAGATCGGG
  ATCCAGGTCACCCCTGATGAAGATGACCACCTACTGTCACCCACGAAAGGCATCTGTCCCAAGCAGGAG
  AACATTGCAGGTAAAGATGAGCCTTCCAGCTACATTTGCACAACATGCAAGCAGCCCTTCAACAGCGCC
  TGGTTCCTGCTGCAGCACGCACAGAACACGCATGGCTTCCGAATCTACCTGGAGCCTGGGCCGGCCAGC
  ACCTCGCTCACGCCCAGGCTCACCATCCCGCCACCGCTCGGGCCGGAGACCGTGGCGCAGTCCCCACTC
  ATGAATTTCCTGGGGGACAGCAATCCTTTCAACCTGCTGCGCATGACGGGCCCCATCCTGCGGGACCAC
  CCTGGCTTCGGTGAGGGCCGCTTGCCAGGTACGCCACCGCTCTTCAGCCCACCGCCACGCCATCACTTG
  GACCCACACCGCCTCAGTGCAGAGGAGATGGGGCTCGTGGCCCAGCACCCCAGTGCCTTCGACCGAGTC
  ATGCGCCTGAACCCCATGGCCATAGACTCTCCTGCCATGGACTTCTCCCGGCGGCTGCGAGAACTGGCC
  GGCAACAGCTCCACGCCGCCGCCCGTGTCCCCAGGCCGTGGCAACCCTATGCACCGGCTGCTGAACCCT
  TTCCAGCCCAGTCCCAAGTCCCCGTTCCTCAGCACGCCACCGCTGCCACCCATGCCTGCGGGCACACCG
  CCACCGCAGCCGCCTGCCAAGAGCAAGTCCTGTGAGTTCTGCGGCAAGACCTTCAAGTTCCAGAGCAAT
  CTCATCGTGCACCGGCGCAGCCACACGGGCGAGAAGCCCTACAAGTGCCAGCTGTGCGACCATGCGTGC
  TCGCAGGCGAGCAAGCTCAAGCGCCACATGAAGACGCACATGCACAAGGCGGGCTCTCTGGCTGGCCGC
  TCAGACGACGGGCTCTCAGCTGCCAGCTCCCCTGAGCCGGGCACCAGCGAGCTGCCAGGTGACCTGAAA
  GCGGCCGATGGCGACTTCCGCCACCATGAGAGCGACCCATCTCTGGGCCCCGAGCCTGAGGACGACGAG
  GACGAGGAGGAGGAAGAAGAGGAGCTGCTGCTGGAGAACGAGAGCCGGCCTGAGTCGAGCTTCAGCATG
  GACTCGGAGCTGGGCCGTGGCCGCGAGAACGGAGGTGGTGTGCCACCGGGGGTGGCGGGCGCAGGGGCT
  GCAGCTGCGGCTCTGGCGGATGAGAAGGCTCTGGCCCTGGGCAAGGTGATGGAGGACGCAGGGCTGGGC
  GCACTGCCGCAGTATGGGGAGAAGCGGGGCGCCTTCCTGAAGCGTGCAGGCGACACGGGTGATGCCGGA
  GCTGTTGGCTGTGGGGACGCGGGTGCACCGGGTGCAGTGAACGGGCGCGGCGGGGCCTTCGCGCCAGGC
  GCAGAGCCCTTTCCAGCTCTCTTCCCACGCAAGCCAGCACCGCTGCCCAGCCCTGGGCTCGGTGGTCCC
  GCGCTGCACGCGGCCAAGCGCATCAAGGTGGAGAAAGACCTGGAGCTGCCACCTGCCGCCCTCATCCCA
  TCTGAGAACGTGTACTCGCAGTGGCTCGTGGGCTACGCAGCATCGCGCCACTTCATGAAGGACCCATTC
  CTGGGCTTCACGGATGCGCGCCAGTCGCCTTTCGCCACATCGTCGGAACATTCCTCTGAGAACGGCAGC
  CTGCGCTTCTCAACGCCACCCGGGGACCTGCTGGACGGCGGGCTGTCCGGGCGCAGTGGCACGGCGAGC
  GGGGGCAGCACACCTCACCTGGGTGGTCCGGGTCCTGGGAGGCCGAGCTCCAAGGAGGGCCGCCGCAGC
  GACACATGTGAGTACTGCGGCAAGGTCTTCAAGAACTGTAGCAACCTGACGGTGCACCGGAGGAGCCAC
  ACCGGCGAGCGGCCTTACAAGTGCGAGCTGTGCAACTACGCGTGTGCGCAGAGCAGCAAGCTCACGCGC
  CACATGAAGACGCACGGGCAGATCGGCAAGGAGGTGTACCGCTGCGACATCTGCCAGATGCCCTTCAGC
  GTCTACAGCACCCTGGAGAAACACATGAAAAAGTGGCACGGTGAACACTTGCTGACTAATGATGTCAAA
  ATCGAGCAGGCTGAGAGGAGC
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGAT
  ATCCTGGATTACAAGGATGACGACGATAAGGTTTAA
  
  Protein Sequence

  >Peptide sequence encoded by MR210782
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MSRRKQGNPQHLSQRELITPEADHVEATILEEDEGLEIEEPSSLGLMVGGPDPDLLTCGQCQMNFPLGD
  ILVFIEHKKKQCGGLGPCYDKVLDKSSPPPSSRSELRRVSEPVEIGIQVTPDEDDHLLSPTKGICPKQE
  NIAGKDEPSSYICTTCKQPFNSAWFLLQHAQNTHGFRIYLEPGPASTSLTPRLTIPPPLGPETVAQSPL
  MNFLGDSNPFNLLRMTGPILRDHPGFGEGRLPGTPPLFSPPPRHHLDPHRLSAEEMGLVAQHPSAFDRV
  MRLNPMAIDSPAMDFSRRLRELAGNSSTPPPVSPGRGNPMHRLLNPFQPSPKSPFLSTPPLPPMPAGTP
  PPQPPAKSKSCEFCGKTFKFQSNLIVHRRSHTGEKPYKCQLCDHACSQASKLKRHMKTHMHKAGSLAGR
  SDDGLSAASSPEPGTSELPGDLKAADGDFRHHESDPSLGPEPEDDEDEEEEEEELLLENESRPESSFSM
  DSELGRGRENGGGVPPGVAGAGAAAAALADEKALALGKVMEDAGLGALPQYGEKRGAFLKRAGDTGDAG
  AVGCGDAGAPGAVNGRGGAFAPGAEPFPALFPRKPAPLPSPGLGGPALHAAKRIKVEKDLELPPAALIP
  SENVYSQWLVGYAASRHFMKDPFLGFTDARQSPFATSSEHSSENGSLRFSTPPGDLLDGGLSGRSGTAS
  GGSTPHLGGPGPGRPSSKEGRRSDTCEYCGKVFKNCSNLTVHRRSHTGERPYKCELCNYACAQSSKLTR
  HMKTHGQIGKEVYRCDICQMPFSVYSTLEKHMKKWHGEHLLTNDVKIEQAERS
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
  
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021399
• ORF Size: 2436 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq Size: 7707 bp
• RefSeq ORF: 2439 bp
• Locus ID: 58208
• UniProt ID: Q99PV8
• Cytogenetics: 12 59.1 cM
• MW: 87.3 kDa
• Gene Summary: Key regulator of both differentiation and survival of T-lymphocytes during thymocyte development in mammals (PubMed:12717433). Essential in controlling the responsiveness of hematopoietic stem cells to chemotactic signals by modulating the expression of receptors CCR7 and CCR9, which direct the movement of progenitor cells from the bone marrow to the thymus (By similarity). Is a regulator of IL2 promoter and enhances IL2 expression in activated CD4(+) T-lymphocytes (PubMed:16809611). Tumor-suppressor protein involved in T-cell lymphomas. May function on the P53-signaling pathway. Repress transcription through direct, TFCOUP2-independent binding to a GC-rich response element.[UniProtKB/Swiss-Prot Function]