Abi3bp (NM_001014424) Mouse Tagged ORF Clone

CAT#: MR215137

Abi3bp (Myc-DDK-tagged) - Mouse ABI gene family, member 3 (NESH) binding protein (Abi3bp), transcript variant 2

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Abi3bp
• Synonyms: 5033411B22Rik; AI506287; BG172926; D930038M13Rik; eratin; TARSH
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR215137 representing NM_001014424
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTCTCCAGCCTGGGGTGTCTACTTCTCTGTGGAAGTATTGCACTTGCTCTGGGAAATGCACAGAAAT
  TGCCAAAAGGTAAAAAGCCAAGCTTGAAAGTGCACATCAATACCACCAGCGACTCCATCCTCTTGAAGTT
  CCTGCGTCCAAATGCAAACGTAAAGCTGGAAGGTTTTCTCCTGGGATATGGCAGCAATGTGTCTCCAAAC
  CAGTACTTCCCTCTTCCCACAGAAGGGAAATTCACAGAGGCTGTAGTTGATGCAGAGCCGAAGTATCTGA
  TAGTCGTACGACCTGCTCCTCCTCCCAGCCAGAAGAAGTCATGTTCAGGGAAATCTCGCCCTCGCAAACC
  CCTCCAGCTGGTGGTGGGTACGTTGACACCGAGTTCTGTCTTCCTGTCCTGGGGATTCCTCATCAACCCA
  CACCATGACTGGACATTGCCAAGCCACTGTCCCAGTGACAGATTTTATACAATTCGATATAGAGAAAAAG
  ATAAAGAAAAGAAATGGATTTTTCAACTCTGCCCAGCTACTGAAACAATTGTGGAAAATCTAAAGCCCAA
  CACAGTTTATGAATTTGGTGTGAAAGACAATATAGAAGGTGGAATTTGGAGTAAGATTTTCAATCATAAG
  ACTATTGTTGGAAGTAAAAACAAAGTAAACGGGAAAATCCAAAGCACTTATGACCAGGTCCACTCAGTGC
  CAGTCCCGAGGAAGCTAATCCCACTAACGATCATTAAGCAAGTGATTCAGAATGTTACTCACAGGGCTTC
  AACCAAATCCCCAGACAAGACTCCTTTTGGGGGAACAATATTAGTTCATCTGATTATTCCGGGTCTTAAT
  GAATCCACTGTAAAACTTCCCACATCCATAATGCTTGAGATTTCCGATGCTCTCAAGGCACAATTAGCTA
  AGAATGAAACATTGGCTTTACCAGCAGAATCTAAAACACCAGAGGTCGAAAAATTGGCAGGGCAGCCAGT
  AACAGTGACTCCGGAATCAGTTTCAAGAAGCACTAAGCCCACCCTGTCTAGTGCTTTAGACACTGCAGAG
  ACAGCACTGGTCCTCAGTGAGAAGACGTCAGAAACAGCCCGCTCTGTTCTAATACCTGAGTTTGAATTGC
  CCTTGAGCACTTTAGCTCCAAAAAGGTTCCCAGAGTTTCCTGAGGCAAAAACAGCCTTCCCATTGGAGAA
  ACCTAGGGGTTCCTGGGCTTCAAGTGAGGAGCCATGGGTGGTACCTGGAGCTAAAACATCTGAAGATTCC
  AGAGTCGTACAGCCTCAAACTGCAACTTATGATGTTATCTCAAGCTCAACAACTTCTGATGAGACTGAAA
  TAGAGATTCACACAGCAACAAGAGATCCTATTCTGGACTCTGTCCCACCTAAAACTTCTAGAACTGCTGA
  ACAGCCAAGGGCAACACTGGCTCCAATCGAAGCGTTATTTGAATCCCGAAATGTGGAAATCTTCACCAGT
  CCTGAAGTGCGACCTACAACGGCTGCTCCTCAGCAAACTACATCTATCCCTTCCACACCCAAACGACAAT
  CAACACCCAAACCGCCAAGAGTCAAGCCTGCACCCAGGCAGACAACCAGCATGCCTCCCAAGCTCAAAAC
  ACCACACTCAAGGATGCCAGCAAAAGAGCCAGTTCCTAAGGAACCCCTGCATACGACTTCAAAGCCAAAA
  ATGCCACCAAGTCCTGAAGTGGCAGACACTACATCTGTTCCAAAAGATGAACGACTTTCCCTTAAACCAG
  ATCCAGAGGTCACTCACAGTGAAACTGTTCTGCCTCCTGTCACCTTTAGAGTTGAGCCACCAAAGACAAC
  AATAGCACCTTTAGAGACACGGGGCATTCCTCTCATACCTGTGATTTCACCAAGACCTAGTCAAGAGGAA
  CTACAAACCGCCATGGAAGAAACAGACCAGTCTACCCAAGAGCTCTTCACGACTAAGATTCCACGAACAA
  CTGAATTGGCAAAGACAACTCAGGCACCACACAGATTGCATACAGCTCCTGTGAGGCCCAGAATACCTGG
  CAGGCCACATGGCAGGCCTGCTCTGAACAAAACAACTACAAGGCCTGATAAAACCAAACCCAGAGGGACG
  TCCCATAAAAACGGAGTAGGAACAGGAACCAAGCAAGCACCTAAGCCACCAAGTCCTGGGAGAAATGCGT
  CAGTGGATTCACATGCCACAAGAAAACCAGGCTCAGTCTCAGGGACGCGTCGTCCACCCATACCACATAG
  GCATTCGTCCACTAGACCTGTGTCTCCAGAGAGAAGGCCTTTACCCCCGAATAATGTCACCGGGAAGCCA
  GGACGTGCGGGAATTGTTTCCTCCAGCCGGGTAACGTCCCCACCCCTGAAGGCAACACTCCACCCTATCG
  GAACAGCCACAGCGAGACCAGGGGCAGAACAAAAGGAACCAACTGCTCCTGCTTCAGAAGAAGAGTTTGG
  TACTACAACTGACTTCAGTTCAAGTCCCACCAAAGAAACTGATCCCCTTGGGAAGCCCAGGTTCATAGGT
  CCCCATGTGCGATATATCCCAAAGCCTGAAAACAAGCCCTGTTCCATCACTGACTCAGTCAGACGGTTCC
  CTACAGAGGAAGCCACGGAGGGGAATGCCACCAGCCCACCACAGAACCCACCCACCAACCTCACTGTGGT
  CACTGTCGAAGGCTGCCCCTCATTTGTCATCTTGGACTGGGAAAAACCTCTAAATGACACTGTCACTGAA
  TATGAAGTCATATCCAGAGAAAATGGGTCATTCAGTGGGAAGAACAAGTCCATTCAAATTACCAATCAAA
  CGTTCTCTACAGTAGAAAACCTGAAACCAGATACAAGCTATGAATTCCAGGTGAAACCCAAAAATCCACT
  TGGTGAGGGCCCCGCCAGCAACACGGTAGCGTTCAGTACTGAATCAGCTGACCCAAGAGTGAGTGAGCCA
  ATTTCTGCAGGAAGAGATGCCATCTGGACTGAAAGACCTTTTAACTCAGACTCTTACTCAGAATGCAAAG
  GCAAACAGTATGTTAAAAGGACGTGGTATAAAAAATTTGTCGGAGTGCAGCTATGTAACTCTCTCAGATA
  CAAGATTTATTTGAGCGATTCCCTCACAGGCAAGTTTTACAACATAGGAGATCAGAGGGGCCACGGAGAA
  GATCACTGCCAGTTTGTGGATTCATTTTTAGATGGTCGCACAGGACAGCAACTCACCTCTGAACAGTTAC
  CCACCAAAGAAGGCTATTTCAGAGCTGTTCGTCAGGAACCTGTCCAATTTGGAGAAATAGGTGGTCATAC
  CCAAATCAATTATGTCCAGTGGTATGAATGTGGGACCACAATACCTGGAAAATGG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR215137 representing NM_001014424
  Red=Cloning site Green=Tags(s)
  
  MLSSLGCLLLCGSIALALGNAQKLPKGKKPSLKVHINTTSDSILLKFLRPNANVKLEGFLLGYGSNVSPN
  QYFPLPTEGKFTEAVVDAEPKYLIVVRPAPPPSQKKSCSGKSRPRKPLQLVVGTLTPSSVFLSWGFLINP
  HHDWTLPSHCPSDRFYTIRYREKDKEKKWIFQLCPATETIVENLKPNTVYEFGVKDNIEGGIWSKIFNHK
  TIVGSKNKVNGKIQSTYDQVHSVPVPRKLIPLTIIKQVIQNVTHRASTKSPDKTPFGGTILVHLIIPGLN
  ESTVKLPTSIMLEISDALKAQLAKNETLALPAESKTPEVEKLAGQPVTVTPESVSRSTKPTLSSALDTAE
  TALVLSEKTSETARSVLIPEFELPLSTLAPKRFPEFPEAKTAFPLEKPRGSWASSEEPWVVPGAKTSEDS
  RVVQPQTATYDVISSSTTSDETEIEIHTATRDPILDSVPPKTSRTAEQPRATLAPIEALFESRNVEIFTS
  PEVRPTTAAPQQTTSIPSTPKRQSTPKPPRVKPAPRQTTSMPPKLKTPHSRMPAKEPVPKEPLHTTSKPK
  MPPSPEVADTTSVPKDERLSLKPDPEVTHSETVLPPVTFRVEPPKTTIAPLETRGIPLIPVISPRPSQEE
  LQTAMEETDQSTQELFTTKIPRTTELAKTTQAPHRLHTAPVRPRIPGRPHGRPALNKTTTRPDKTKPRGT
  SHKNGVGTGTKQAPKPPSPGRNASVDSHATRKPGSVSGTRRPPIPHRHSSTRPVSPERRPLPPNNVTGKP
  GRAGIVSSSRVTSPPLKATLHPIGTATARPGAEQKEPTAPASEEEFGTTTDFSSSPTKETDPLGKPRFIG
  PHVRYIPKPENKPCSITDSVRRFPTEEATEGNATSPPQNPPTNLTVVTVEGCPSFVILDWEKPLNDTVTE
  YEVISRENGSFSGKNKSIQITNQTFSTVENLKPDTSYEFQVKPKNPLGEGPASNTVAFSTESADPRVSEP
  ISAGRDAIWTERPFNSDSYSECKGKQYVKRTWYKKFVGVQLCNSLRYKIYLSDSLTGKFYNIGDQRGHGE
  DHCQFVDSFLDGRTGQQLTSEQLPTKEGYFRAVRQEPVQFGEIGGHTQINYVQWYECGTTIPGKW
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001014424
• ORF Size: 3345 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001014424.1, NM_001014424.2, NP_001014424.1
• RefSeq Size: 4502 bp
• RefSeq ORF: 3348 bp
• Locus ID: 320712
• Cytogenetics: 16 C1.1
• MW: 122.9 kDa