Aanat (NM_009591) Mouse Tagged ORF Clone

CAT#: MR218438

Aanat (Myc-DDK-tagged) - Mouse arylalkylamine N-acetyltransferase (Aanat), transcript variant 1

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Aanat
• Synonyms: AA-NAT; Nat-2; Nat4; Snat
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR218438 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTGAACATCAACTCCCTGAAACCTGAGGCCCTGCACCTGCCACTTGGGACCTCAGAGTTCCTAGGCT
  GCCAGCGGCGCCACACACTCCCTGCCAGTGAGTTCCGCTGCCTCACACCTGAGGATGCCACCAGTGCGTT
  TGAGATTGAGCGGGAAGCCTTTATCTCTGTCTCCGGTACCTGTCCCCTCTACTTGGATGAGATCCGGCAC
  TTCCTAACCCTGTGTCCAGAGCTGTCACTGGGCTGGTTTGAGGAAGGCTGCCTTGTGGCCTTCATCATTG
  GCTCGCTGTGGGACAAGGAGAGACTTACTCAGGAGTCGCTGACACTACACAGGCCCGGAGGCCGCACAGC
  CCACCTGCACGTACTGGCGGTGCACAGAACCTTCCGGCAGCAGGGCAAGGGCTCTGTCCTCCTGTGGAGA
  TACCTTCACCACCTGGGCAGTCAGCCGGCCGTGCGCCGGGCTGTGCTCATGTGTGAGGATGCCCTGGTAC
  CCTTCTATGAGAAATTTGGCTTCCAGGCTGTGGGCCCATGTGCCATCACCGTGGGCTCTCTCACCTTCAC
  GGAGCTACAGTGTTCCTTACGATGCCACGCCTTCCTGCGCAGGAACAGCGGCTGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR218438 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MLNINSLKPEALHLPLGTSEFLGCQRRHTLPASEFRCLTPEDATSAFEIEREAFISVSGTCPLYLDEIRH
  FLTLCPELSLGWFEEGCLVAFIIGSLWDKERLTQESLTLHRPGGRTAHLHVLAVHRTFRQQGKGSVLLWR
  YLHHLGSQPAVRRAVLMCEDALVPFYEKFGFQAVGPCAITVGSLTFTELQCSLRCHAFLRRNSGC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009591
• ORF Size: 618 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_009591.1, NM_009591.2, NM_009591.3, NP_033721.1
• RefSeq Size: 1370
• RefSeq ORF: 618
• Locus ID: 11298
• MW: 23.1 kDa
• Gene Summary: The protein encoded by this gene belongs to the acetyltransferase superfamily. It is the penultimate enzyme in melatonin synthesis and controls the night/day rhythm in melatonin production in the vertebrate pineal gland. Melatonin is essential for the function of the circadian clock that influences activity and sleep. This enzyme is regulated by cAMP-dependent phosphorylation that promotes its interaction with 14-3-3 proteins and thus protects the enzyme against proteasomal degradation. This gene may contribute to numerous genetic diseases such as delayed sleep phase syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2010]