Fgfr2 (NM_201601) Mouse Tagged ORF Clone

CAT#: MR225121

Fgfr2 (Myc-DDK-tagged) - Mouse fibroblast growth factor receptor 2 (Fgfr2), transcript variant 2

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Fgfr2
• Synonyms: AU043015; AW556123; Bek; Fgfr-2; Fgfr-7; Fgfr7; KGFR; KGFRTr; svs
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR225121 representing NM_201601.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGGATTACCGTCCACGTGGAGATATGGAAGAGGACCAGGGATTGGCACTGTGACCATGGTCAGCTGG
  GGGCGCTTCATCTGCCTGGTCTTGGTCACCATGGCAACCTTGTCCCTGGCCCGGCCCTCCTTCAGTTTA
  GTTGAGGATACCACTTTAGAACCAGAAGGAGCACCGTACTGGACCAACACCGAGAAGATGGAGAAGCGG
  CTCCACGCTGTCCCTGCCGCCAACACTGTGAAGTTCCGCTGTCCGGCTGGGGGGAATCCAACGCCCACA
  ATGAGGTGGTTAAAAAACGGGAAGGAGTTTAAGCAGGAGCATCGCATTGGAGGCTATAAGGTACGAAAC
  CAGCACTGGAGCCTTATTATGGAAAGTGTGGTCCCGTCAGACAAAGGCAACTACACCTGCCTGGTGGAG
  AATGAATACGGGTCCATCAACCACACCTACCACCTCGATGTCGTTGAACGGTCACCACACCGGCCCATC
  CTCCAAGCTGGACTGCCTGCAAATGCCTCCACGGTGGTCGGAGGGGATGTGGAGTTTGTCTGCAAGGTT
  TACAGCGATGCCCAGCCCCACATCCAGTGGATCAAGCACGTGGAAAAGAACGGCAGTAAATACGGGCCT
  GATGGGCTGCCCTACCTCAAGGTCCTGAAGCACTCGGGGATAAATAGCTCCAATGCAGAAGTGCTGGCT
  CTGTTCAATGTGACGGAGATGGATGCTGGGGAATATATATGTAAGGTCTCCAATTATATAGGGCAGGCC
  AACCAGTCTGCCTGGCTCACTGTCCTGCCCAAACAGCAAGCGCCTGTGAGAGAGAAGGAGATCACGGCT
  TCCCCAGATTATCTGGAGATAGCTATTTACTGCATAGGGGTCTTCTTAATCGCCTGCATGGTGGTGACA
  GTCATCTTTTGCCGAATGAAGACCACGACCAAGAAGCCAGACTTCAGCAGCCAGCCAGCTGTGCACAAG
  CTGACCAAGCGCATCCCCCTGCGGAGACAGGTAACAGTTTCGGCCGAGTCCAGCTCCTCCATGAACTCC
  AACACCCCGCTGGTGAGGATAACAACGCGTCTGTCCTCAACAGCGGACACCCCGATGCTAGCAGGGGTC
  TCCGAGTATGAGTTGCCAGAGGATCCAAAGTGGGAATTCCCCAGAGATAAGCTGACGCTGGGCAAACCC
  CTGGGGGAAGGTTGCTTCGGGCAAGTAGTCATGGCTGAAGCAGTGGGAATCGATAAAGACAAACCCAAG
  GAGGCGGTCACCGTGGCAGTGAAGATGTTGAAAGATGATGCCACAGAGAAGGACCTGTCTGATCTGGTA
  TCAGAGATGGAGATGATGAAGATGATTGGGAAACATAAGAACATTATCAACCTCCTGGGGGCCTGCACG
  CAGGATGGACCTCTCTACGTCATAGTTGAATATGCATCGAAAGGCAACCTCCGGGAATACCTCCGAGCC
  CGGAGGCCACCTGGCATGGAGTACTCCTATGACATTAACCGTGTCCCCGAGGAGCAGATGACCTTCAAG
  GACTTGGTGTCCTGCACCTACCAGCTGGCTAGAGGCATGGAGTACTTGGCTTCCCAAAAATGTATCCAT
  CGAGATTTGGCTGCCAGAAACGTGTTGGTAACAGAAAACAATGTGATGAAGATAGCAGACTTTGGCCTG
  GCCAGGGATATCAACAACATAGACTACTATAAAAAGACCACAAATGGGCGACTTCCAGTCAAGTGGATG
  GCTCCTGAAGCCCTTTTTGATAGAGTTTACACTCATCAGAGCGATGTCTGGTCCTTCGGGGTGTTAATG
  TGGGAGATCTTTACTTTAGGGGGCTCACCCTACCCAGGGATTCCCGTGGAGGAACTTTTTAAGCTGCTC
  AAAGAGGGACACAGGATGGACAAGCCCACCAACTGCACCAATGAACTGTACATGATGATGAGGGATTGC
  TGGCATGCTGTACCCTCACAGAGACCCACATTCAAGCAGTTGGTCGAAGACTTGGATCGAATTCTGACT
  CTCACAACCAATGAGGAATACTTGGATCTCACCCAGCCTCTCGAACAGTATTCTCCTAGTTACCCCGAC
  ACAAGGAGCTCTTGTTCTTCAGGGGACGATTCTGTGTTTTCTCCAGACCCCATGCCTTATGAACCCTGT
  CTGCCTCAGTATCCACACATAAACGGCAGTGTTAAAACA
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGAT
  ATCCTGGATTACAAGGATGACGACGATAAGGTTTAA
  
  Protein Sequence

  >Peptide sequence encoded by MR225121
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MGLPSTWRYGRGPGIGTVTMVSWGRFICLVLVTMATLSLARPSFSLVEDTTLEPEGAPYWTNTEKMEKR
  LHAVPAANTVKFRCPAGGNPTPTMRWLKNGKEFKQEHRIGGYKVRNQHWSLIMESVVPSDKGNYTCLVE
  NEYGSINHTYHLDVVERSPHRPILQAGLPANASTVVGGDVEFVCKVYSDAQPHIQWIKHVEKNGSKYGP
  DGLPYLKVLKHSGINSSNAEVLALFNVTEMDAGEYICKVSNYIGQANQSAWLTVLPKQQAPVREKEITA
  SPDYLEIAIYCIGVFLIACMVVTVIFCRMKTTTKKPDFSSQPAVHKLTKRIPLRRQVTVSAESSSSMNS
  NTPLVRITTRLSSTADTPMLAGVSEYELPEDPKWEFPRDKLTLGKPLGEGCFGQVVMAEAVGIDKDKPK
  EAVTVAVKMLKDDATEKDLSDLVSEMEMMKMIGKHKNIINLLGACTQDGPLYVIVEYASKGNLREYLRA
  RRPPGMEYSYDINRVPEEQMTFKDLVSCTYQLARGMEYLASQKCIHRDLAARNVLVTENNVMKIADFGL
  ARDINNIDYYKKTTNGRLPVKWMAPEALFDRVYTHQSDVWSFGVLMWEIFTLGGSPYPGIPVEELFKLL
  KEGHRMDKPTNCTNELYMMMRDCWHAVPSQRPTFKQLVEDLDRILTLTTNEEYLDLTQPLEQYSPSYPD
  TRSSCSSGDDSVFSPDPMPYEPCLPQYPHINGSVKT
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
  
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_201601
• ORF Size: 2178 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_201601.2, NP_963895.2
• RefSeq Size: 4881 bp
• RefSeq ORF: 2181 bp
• Locus ID: 14183
• UniProt ID: P21803
• Cytogenetics: 7 F3
• MW: 81.6 kDa
• Gene Summary: Tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of cell proliferation, differentiation, migration and apoptosis, and in the regulation of embryonic development. Required for normal embryonic patterning, trophoblast function, limb bud development, lung morphogenesis, osteogenesis and skin development. Plays an essential role in the regulation of osteoblast differentiation, proliferation and apoptosis, and is required for normal skeleton development. Promotes cell proliferation in keratinocytes and immature osteoblasts, but promotes apoptosis in differentiated osteoblasts. Phosphorylates PLCG1, FRS2 and PAK4. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. FGFR2 signaling is down-regulated by ubiquitination, internalization and degradation. Mutations that lead to constitutive kinase activation or impair normal FGFR2 maturation, internalization and degradation lead to aberrant signaling. Over-expressed FGFR2 promotes activation of STAT1.[UniProtKB/Swiss-Prot Function]