Cldn16 (NM_053241) Mouse Tagged ORF Clone

CAT#: MR225210

Cldn16 (Myc-DDK-tagged) - Mouse claudin 16 (Cldn16)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cldn16
• Synonyms: claudin-16; PCLN1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR225210 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGGATCTTCTTCAGTACGCTGCCTGCTTCTTGGCCATATTCTCCACTGGGTTTTTGATCGTGGCCA
  CCTGGACAGACTGTTGGATGGTGAACGCTGATGACTCCCTGGAGGTGAGCACTAAATGCAGAGGCCTGTG
  GTGGGAGTGTGTAACAAACGCTTTTGATGGGATTCGAACCTGCGATGAGTACGACTCCATATATGCAGAA
  CATCCCTTGAAGCTGGTGGTAACTCGAGCACTGATGATCACAGCTGACATTTTAGCTGGCTTTGGATTCA
  TCACCCTGCTCCTTGGTCTGGACTGTGTGAAGTTCCTACCTGATGACCCACAAATTAAAGTCCGCCTTTG
  CTTTGTTGCAGGGACCACATTACTCATTGCAGGTACCCCAGGAATCATCGGTTCTGTGTGGTATGCTGTG
  GATGTTTACGTCGAACGCTCCTCTCTCGTTTTACACAATATATTTCTTGGGATCCAATATAAATTTGGTT
  GGTCCTGCTGGCTTGGAATGGCTGGGTCTTTGGGTTGCTTTTTGGCAGGAGCTCTCCTCACCTGCTGTTT
  GTACCTCTTCAAAGATGTTGGGCCTGAGAGGAACTACCCTTATGCCATGAGGAAGCCCTATTCAACTGCA
  GGTGTGTCCATGGCCAAGTCCTACAAGGCCCCTCGGACAGAGACAGCCAAAATGTATGCTGTAGACACCA
  GAGTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR225210 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MKDLLQYAACFLAIFSTGFLIVATWTDCWMVNADDSLEVSTKCRGLWWECVTNAFDGIRTCDEYDSIYAE
  HPLKLVVTRALMITADILAGFGFITLLLGLDCVKFLPDDPQIKVRLCFVAGTTLLIAGTPGIIGSVWYAV
  DVYVERSSLVLHNIFLGIQYKFGWSCWLGMAGSLGCFLAGALLTCCLYLFKDVGPERNYPYAMRKPYSTA
  GVSMAKSYKAPRTETAKMYAVDTRV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_053241
• ORF Size: 708 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_053241.1, NM_053241.2, NM_053241.3, NM_053241.4, NM_053241.5, NP_444471.1
• RefSeq Size: 1173
• RefSeq ORF: 708
• Locus ID: 114141
• MW: 26.1 kDa
• Gene Summary: This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. The protein encoded by this gene is critical for renal paracellular epithelial transport of Ca(2+) and Mg(2+) in the thick ascending loop of Henle. The gene deficiency leads to specific alterations in renal Ca(2+) and Mg(2+) balance and also to disturbances in Na(+) handling. The interaction of this gene and the Cldn 19 gene is required for their assembly into tight junctions and for renal Mg(2+) reabsorption. This gene and the Cldn1 gene are clustered on chromosome 16. [provided by RefSeq, Aug 2010]