F8 (NM_001161374) Mouse Tagged ORF Clone

CAT#: MR225868

F8 (Myc-DDK-tagged) - Mouse coagulation factor VIII (F8), transcript variant 3

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: F8
• Synonyms: Cf-8; Cf8; FVIII
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR225868 representing NM_001161374
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAAATAGCACTCTTCGCTTGCTTCTTTCTGAGCCTTTTCAATTTCTGCTCTAGTGCCATCAGAAGAT
  ACTACCTTGGTGCAGTGGAATTGTCCTGGAACTATATTCAGAGTGATCTGCTCAGTGTGCTGCATACAGA
  CTCAAGATTTCTTCCTAGAATGTCAACATCTTTTCCATTCAACACCTCCATCATGTATAAAAAGACTGTG
  TTTGTAGAGTACAAGGACCAGCTTTTCAACATTGCCAAGCCCAGGCCACCCTGGATGGGTTTGCTAGGTC
  CTACCATTTGGACTGAGGTTCATGACACAGTGGTCATTACACTTAAAAACATGGCTTCTCATCCTGTCAG
  TCTTCATGCTGTTGGTGTGTCCTACTGGAAAGCTTCTGAGGGAGATGAATATGAAGATCAGACAAGCCAA
  ATGGAGAAGGAAGATGATAAAGTTTTCCCTGGTGAAAGTCATACTTATGTTTGGCAAGTCCTGAAAGAGA
  ATGGTCCAATGGCCTCTGACCCTCCATGTCTCACTTACTCATATATGTCTCATGTGGATCTGGTGAAAGA
  TTTGAATTCAGGCCTCATTGGAGCTCTGCTAGTATGTAAAGAAGGCAGTCTCTCCAAAGAAAGAACACAG
  ATGTTGTACCAATTTGTACTGCTTTTTGCTGTATTTGATGAAGGGAAGAGCTGGCACTCAGAAACAAACG
  ACTCTTATACACAGTCTATGGATTCTGCATCTGCTAGAGACTGGCCTAAAATGCACACAGTCAATGGCTA
  TGTAAACAGGTCTCTTCCAGGTCTGATTGGATGCCATAGGAAATCAGTCTACTGGCACGTGATTGGAATG
  GGCACCACTCCTGAAATACACTCAATATTCCTCGAAGGTCACACATTTTTTGTGAGGAACCACCGTCAAG
  CTTCATTGGAGATATCACCAATAACTTTCCTTACTGCTCAAACACTCTTGATAGATCTTGGGCAGTTCCT
  ACTATTTTGTCATATCTCTTCCCATAAACATGATGGCATGGAAGCTTATGTCAAAGTAGATAGCTGCCCT
  GAGGAATCCCAATGGCAAAAGAAAAATAATAATGAGGAAATGGAAGATTATGATGATGATCTTTATTCAG
  AAATGGATATGTTCACATTGGATTATGACAGCTCTCCTTTTATCCAAATTCGCTCGGTTGCTAAAAAGTA
  CCCTAAAACTTGGATACATTATATTTCTGCTGAGGAGGAAGACTGGGACTATGCACCTTCAGTTCCTACC
  TCGGATAATGGAAGTTATAAAAGCCAGTATCTGAGCAATGGTCCTCATCGGATTGGTAGGAAATATAAAA
  AAGTCAGATTTATAGCATACACAGATGAAACCTTTAAGACTCGTGAAACTATTCAGCATGAATCAGGACT
  CTTGGGACCTTTACTTTATGGAGAAGTTGGAGACACACTGTTGATTATTTTTAAGAATCAAGCAAGCCGA
  CCATATAACATTTACCCTCATGGAATCACTGATGTCAGTCCTCTACATGCAAGGAGATTGCCAAGAGGTA
  TAAAGCACGTGAAGGATTTGCCAATTCATCCAGGAGAGATATTCAAGTACAAGTGGACAGTTACAGTAGA
  AGATGGACCAACTAAATCAGATCCACGGTGCCTGACCCGCTATTATTCAAGTTTCATTAACCCTGAGAGA
  GATCTAGCTTCAGGACTGATTGGCCCTCTTCTCATCTGCTACAAAGAATCTGTAGATCAAAGGGGAAACC
  AGATGATGTCAGACAAAAGAAATGTCATCCTGTTTTCTATATTTGATGAGAACCAAAGCTGGTACATCAC
  AGAGAACATGCAACGCTTCCTCCCCAATGCAGCTAAAACACAGCCCCAGGACCCTGGGTTCCAGGCCTCC
  AACATCATGCACAGTCTATGGGTCTTGGGGTGTCATAATTCAGACTTTCGGAAGAGAGGTATGACAGCAT
  TGCTGAAAGTTTCTAGTTGTGACAAGAGCACTAGTGATTATTATGAAGAAATATATGAAGATATTCCAAC
  ACAGTTGGTGAATGAGAACAATGTCATTGATCCCAGAAGCTTCTTCCAGAATACAAATCATCCTAATACT
  AGGAAAAAGAAATTCAAAGATTCCACAATTCCAAAAAATGATATGGAGAAGATTGAGCCTCAGTTTGAAG
  AGATAGCAGAGATGCTTAAAGTACAGAGTGTCTCAGTTAGTGACATGTTGATGCTCTTGGGACAGAGTCA
  TCCTACTCCACATGGCTTATTTTTATCAGATGGCCAAGAAGCCATCTATGAGGCTATTCATGATGATCAT
  TCACCAAATGCAATAGACAGCAATGAAGGCCCATCTAAAGTGACCCAACTCAGGCCAGAATCCCATCACA
  GTGAGAAAATAGTATTTACTCCTCAGCCCGGCCTCCAGTTAAGATCCAATAAAAGTTTGGAGACAACTAT
  AGAAGTAAAGTGGAAGAAACTTGGTTTGCAAGTTTCTAGTTTGCCAAGTAATCTAATGACTACAACAATT
  CTGTCAGACAATTTGAAAGCAACTTTTGAAAAGACAGATTCTTCAGGATTTCCAGATATGCCAGTTCACT
  CTAGTAGTAAATTAAGTACTACTGCATTTGGTAAGAAAGCATATTCCCTTGTTGGGTCTCATGTACCTTT
  AAACGTGAGTGAAGAAAATAGTGATTCCAACATATTGGATTCAACTTTAATGTATAGTCAAGAAAGTTTA
  CCAAGAGATAATATATTATCAATGGAGAATGATAGATTACTCAGAGAGAAGAGGTTTCATGGAATTGCTT
  TATTGACCAAAGATAATACTTTATTCAAAGACAATGTCTCCTTAATGAAAACAAACAAAACATATAATCA
  TTCAACAACTAATGAAAAACTACACACTGAGAGCCCAACATCAATTGAGAATAGTACAACAGACTTGCAA
  GATGCCATATTAAAGGTCAATAGTGAGATTCAAGAAGTAACAGCTTTGATTCATGATGGAACACTTTTAG
  GCAAAAATTCTACATATTTGAGACTAAACCATATGCTAAATAGAACTACCTCAACAAAAAATAAAGACAT
  ATTTCATAGAAAAGATGAAGATCCTATTCCACAAGATGAAGAGAATACAATCATGCCATTTTCCAAGATG
  TTGTTCTTGTCAGAATCTTCAAATTGGTTTAAAAAGACCAATGGAAATAATTCCTTGAACTCTGAGCAAG
  AACATAGTCCAAAGCAATTAGTATATTTAATGTTTAAAAAATATGTAAAAAATCAAAGTTTCTTGTCAGA
  GAAAAATAAAGTCACAGTAGAACAGGATGGATTTACAAAGAACATAGGACTTAAAGACATGGCTTTTCCA
  CATAATATGAGCATATTTCTTACCACTTTGTCTAACGTACATGAAAATGGTAGGCACAATCAAGAAAAAA
  ATATTCAGGAAGAGATAGAGAAGGAAGCACTAATTGAAGAGAAAGTAGTTTTGCCCCAGGTGCACGAAGC
  AACTGGCTCTAAGAATTTCTTGAAAGACATATTGATACTAGGCACTAGGCAAAATATAAGTTTATATGAA
  GTACATGTACCAGTACTTCAAAACATCACATCAATAAACAATTCAACAAATACAGTACAGATTCACATGG
  AGCATTTCTTTAAAAGAAGGAAGGACAAGGAAACAAATTCAGAAGGCTTGGTAAATAAAACCAGAGAAAT
  GGTAAAAAACTATCCAAGCCAGAAGAATATTACTACTCAACGTAGTAAACGGGCTTTGGGACAATTCAGA
  CTGTCAACTCAATGGCTTAAAACCATAAACTGTTCAACACAGTGTATCATTAAACAGATAGACCACAGCA
  AGGAAATGAAAAAGTTCATTACTAAATCTTCCTTATCAGATTCTTCTGTGATTAAAAGCACCACTCAGAC
  AAATAGTTCTGACTCACACATTGTAAAAACATCAGCATTTCCACCAATAGATCTCAAAAGGAGTCCATTC
  CAAAACAAATTTTCTCATGTTCAAGCATCATCCTACATTTATGACTTTAAGACAAAAAGTTCAAGAATTC
  AAGAAAGCAATAATTTCTTAAAAGAAACCAAAATAAATAACCCTTCTTTAGCCATTCTACCATGGAATAT
  GTTCATAGATCAAGGAAAATTTACCTCCCCAGGGAAAAGTAACACAAACTCAGTCACATATAAGAAACGT
  GAGAACATTATTTTCTTGAAACCAACTTTGCCTGAAGAATCTGGCAAAATTGAATTGCTTCCTCAAGTTT
  CCATTCAAGAGGAAGAAATTTTACCTACAGAAACTAGCCATGGATCTCCTGGACACTTGAATCTCATGAA
  AGAGGTCTTTCTTCAGAAAATACAGGGGCCTACTAAATGGAATAAAGCAAAGAGGCATGGAGAAAGTATA
  AAAGGTAAAACAGAGAGCTCTAAAAATACTCGCTCAAAACTGCTAAATCATCATGCTTGGGATTATCATT
  ATGCTGCACAGATACCAAAAGATATGTGGAAATCCAAAGAGAAGTCACCAGAAATTATATCCATTAAGCA
  AGAGGACACCATTTTGTCTCTGAGGCCTCATGGAAACAGTCATTCAATAGGGGCAAATGAGAAACAAAAT
  TGGCCTCAAAGAGAAACCACTTGGGTAAAGCAAGGCCAAACTCAAAGGACATGCTCTCAAATCCCACCAG
  TGTTGAAACGACATCAAAGGGAACTTAGTGCTTTTCAATCAGAACAAGAAGCAACTGACTATGATGATGC
  CATCACCATTGAAACAATCGAGGATTTTGACATTTACAGTGAGGACATAAAGCAAGGTCCCCGCAGCTTT
  CAACAGAAAACAAGGCACTATTTTATTGCAGCTGTGGAACGACTCTGGGACTATGGGATGAGTACATCTC
  ATGTTCTACGAAATAGGTATCAAAGTGACAATGTACCTCAGTTCAAGAAAGTAGTTTTCCAGGAATTTAC
  TGATGGCTCCTTTAGTCAGCCCTTATATCGTGGAGAATTAAATGAACACCTGGGGTTGTTGGGCCCATAT
  ATAAGAGCAGAAGTTGAAGACAACATTATGGTAACTTTCAAAAACCAGGCCTCCCGTCCCTACTCCTTCT
  ATTCTAGCCTCATTTCTTATAAAGAAGATCAGAGAGGAGAAGAACCTAGAAGAAACTTTGTCAAGCCTAA
  TGAAACCAAAATTTATTTTTGGAAAGTACAACATCATATGGCACCCACAGAAGATGAGTTTGACTGCAAG
  GCCTGGGCTTATTTCTCTGATGTTGATCTTGAAAGAGATATGCACTCGGGATTAATTGGACCCCTTCTGA
  TTTGCCACGCGAACACACTGAATCCTGCTCATGGGAGACAAGTGTCAGTACAGGAATTTGCTCTGCTTTT
  CACTATCTTTGATGAGACCAAGAGCTGGTACTTCACTGAAAACGTGAAAAGGAACTGCAAGACACCCTGC
  AATTTCCAGATGGAAGACCCCACTTTGAAAGAGAATTATCGCTTCCATGCAATCAATGGTTATGTAATGG
  ATACCCTACCAGGCTTAGTAATGGCTCAAGATCAAAGGATTCGATGGTATCTTCTCAGCATGGGCAACAA
  TGAGAACATCCAATCTATTCATTTCAGTGGACATGTTTTCACTGTACGGAAAAAAGAGGAGTATAAAATG
  GCAGTGTACAACCTCTACCCAGGTGTTTTTGAGACTCTGGAAATGATACCATCCAGAGCTGGAATATGGC
  GAGTAGAATGCCTTATTGGCGAGCACTTACAGGCTGGGATGAGCACTCTTTTTCTGGTGTACAGCAAGCA
  GTGTCAGATTCCTCTTGGAATGGCTTCTGGAAGCATCCGTGATTTCCAGATTACAGCTTCAGGACATTAT
  GGACAGTGGGCCCCAAACCTGGCAAGACTTCATTATTCCGGATCAATCAATGCCTGGAGTACCAAGGAGC
  CCTTTTCTTGGATCAAGGTAGATCTGTTGGCACCAATGATTGTTCATGGCATCAAGACTCAGGGTGCTCG
  TCAGAAATTTTCCAGCCTTTATATCTCTCAATTTATCATCATGTATAGCCTGGATGGGAAGAAGTGGCTG
  AGTTATCAAGGAAATTCCACTGGAACCTTAATGGTTTTCTTTGGCAATGTGGACTCATCTGGGATTAAGC
  ATAATAGTTTTAATCCTCCAATTATTGCTCGATATATCCGTTTGCACCCCACTCATTCTAGCATCCGTAG
  TACTCTTCGCATGGAGTTGATGGGCTGTGATTTAAACAGTTGCAGCATACCATTGGGAATGGAAAGTAAA
  GTAATATCAGATACACAAATCACTGCCTCATCCTACTTCACCAACATGTTTGCTACTTGGTCTCCTTCAC
  AAGCTCGACTTCACCTCCAGGGAAGGACTAATGCCTGGCGACCTCAGGTGAATGATCCAAAACAATGGTT
  GCAAGTGGACTTACAAAAGACAATGAAAGTCACTGGAATAATAACCCAGGGAGTGAAATCTCTCTTTACC
  AGCATGTTTGTGAAAGAGTTCCTTATTTCCAGCAGTCAAGATGGCCATCACTGGACTCAAATTTTATACA
  ATGGCAAGGTAAAGGTTTTTCAGGGGAATCAGGACTCATCCACACCTATGATGAATTCTCTAGACCCACC
  ATTACTCACTCGCTATCTTCGAATTCACCCCCAGATCTGGGAGCACCAAATTGCTCTGAGGCTTGAGATT
  CTAGGATGTGAGGCCCAGCAGCAATAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR225868 representing NM_001161374
  Red=Cloning site Green=Tags(s)
  
  MQIALFACFFLSLFNFCSSAIRRYYLGAVELSWNYIQSDLLSVLHTDSRFLPRMSTSFPFNTSIMYKKTV
  FVEYKDQLFNIAKPRPPWMGLLGPTIWTEVHDTVVITLKNMASHPVSLHAVGVSYWKASEGDEYEDQTSQ
  MEKEDDKVFPGESHTYVWQVLKENGPMASDPPCLTYSYMSHVDLVKDLNSGLIGALLVCKEGSLSKERTQ
  MLYQFVLLFAVFDEGKSWHSETNDSYTQSMDSASARDWPKMHTVNGYVNRSLPGLIGCHRKSVYWHVIGM
  GTTPEIHSIFLEGHTFFVRNHRQASLEISPITFLTAQTLLIDLGQFLLFCHISSHKHDGMEAYVKVDSCP
  EESQWQKKNNNEEMEDYDDDLYSEMDMFTLDYDSSPFIQIRSVAKKYPKTWIHYISAEEEDWDYAPSVPT
  SDNGSYKSQYLSNGPHRIGRKYKKVRFIAYTDETFKTRETIQHESGLLGPLLYGEVGDTLLIIFKNQASR
  PYNIYPHGITDVSPLHARRLPRGIKHVKDLPIHPGEIFKYKWTVTVEDGPTKSDPRCLTRYYSSFINPER
  DLASGLIGPLLICYKESVDQRGNQMMSDKRNVILFSIFDENQSWYITENMQRFLPNAAKTQPQDPGFQAS
  NIMHSLWVLGCHNSDFRKRGMTALLKVSSCDKSTSDYYEEIYEDIPTQLVNENNVIDPRSFFQNTNHPNT
  RKKKFKDSTIPKNDMEKIEPQFEEIAEMLKVQSVSVSDMLMLLGQSHPTPHGLFLSDGQEAIYEAIHDDH
  SPNAIDSNEGPSKVTQLRPESHHSEKIVFTPQPGLQLRSNKSLETTIEVKWKKLGLQVSSLPSNLMTTTI
  LSDNLKATFEKTDSSGFPDMPVHSSSKLSTTAFGKKAYSLVGSHVPLNVSEENSDSNILDSTLMYSQESL
  PRDNILSMENDRLLREKRFHGIALLTKDNTLFKDNVSLMKTNKTYNHSTTNEKLHTESPTSIENSTTDLQ
  DAILKVNSEIQEVTALIHDGTLLGKNSTYLRLNHMLNRTTSTKNKDIFHRKDEDPIPQDEENTIMPFSKM
  LFLSESSNWFKKTNGNNSLNSEQEHSPKQLVYLMFKKYVKNQSFLSEKNKVTVEQDGFTKNIGLKDMAFP
  HNMSIFLTTLSNVHENGRHNQEKNIQEEIEKEALIEEKVVLPQVHEATGSKNFLKDILILGTRQNISLYE
  VHVPVLQNITSINNSTNTVQIHMEHFFKRRKDKETNSEGLVNKTREMVKNYPSQKNITTQRSKRALGQFR
  LSTQWLKTINCSTQCIIKQIDHSKEMKKFITKSSLSDSSVIKSTTQTNSSDSHIVKTSAFPPIDLKRSPF
  QNKFSHVQASSYIYDFKTKSSRIQESNNFLKETKINNPSLAILPWNMFIDQGKFTSPGKSNTNSVTYKKR
  ENIIFLKPTLPEESGKIELLPQVSIQEEEILPTETSHGSPGHLNLMKEVFLQKIQGPTKWNKAKRHGESI
  KGKTESSKNTRSKLLNHHAWDYHYAAQIPKDMWKSKEKSPEIISIKQEDTILSLRPHGNSHSIGANEKQN
  WPQRETTWVKQGQTQRTCSQIPPVLKRHQRELSAFQSEQEATDYDDAITIETIEDFDIYSEDIKQGPRSF
  QQKTRHYFIAAVERLWDYGMSTSHVLRNRYQSDNVPQFKKVVFQEFTDGSFSQPLYRGELNEHLGLLGPY
  IRAEVEDNIMVTFKNQASRPYSFYSSLISYKEDQRGEEPRRNFVKPNETKIYFWKVQHHMAPTEDEFDCK
  AWAYFSDVDLERDMHSGLIGPLLICHANTLNPAHGRQVSVQEFALLFTIFDETKSWYFTENVKRNCKTPC
  NFQMEDPTLKENYRFHAINGYVMDTLPGLVMAQDQRIRWYLLSMGNNENIQSIHFSGHVFTVRKKEEYKM
  AVYNLYPGVFETLEMIPSRAGIWRVECLIGEHLQAGMSTLFLVYSKQCQIPLGMASGSIRDFQITASGHY
  GQWAPNLARLHYSGSINAWSTKEPFSWIKVDLLAPMIVHGIKTQGARQKFSSLYISQFIIMYSLDGKKWL
  SYQGNSTGTLMVFFGNVDSSGIKHNSFNPPIIARYIRLHPTHSSIRSTLRMELMGCDLNSCSIPLGMESK
  VISDTQITASSYFTNMFATWSPSQARLHLQGRTNAWRPQVNDPKQWLQVDLQKTMKVTGIITQGVKSLFT
  SMFVKEFLISSSQDGHHWTQILYNGKVKVFQGNQDSSTPMMNSLDPPLLTRYLRIHPQIWEHQIALRLEI
  LGCEAQQQY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001161374
• ORF Size: 6741 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001161374.1, NP_001154846.1
• RefSeq Size: 7306
• RefSeq ORF: 6744
• Locus ID: 14069
• MW: 258.4 kDa
• Gene Summary: Factor VIII, along with calcium and phospholipid, acts as a cofactor for factor IXa when it converts factor X to the activated form, factor Xa. [UniProtKB/Swiss-Prot Function]