Ache (NM_009599) Mouse Tagged ORF Clone

CAT#: MR226310

Ache (Myc-DDK-tagged) - Mouse acetylcholinesterase (Ache)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ache
• Synonyms: mE1a; mE1b; mE1c; mE1c-long; mE1d; mE1d'; mE1e
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR226310 representing NM_009599
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGCCTCCCTGGTATCCCCTGCATACACCTTCCCTGGCTTTTCCACTCCTCTTCCTCCTCCTCTCCC
  TCCTGGGAGGAGGGGCAAGGGCTGAGGGCCGGGAAGACCCGCAGCTGCTGGTGAGGGTTCGAGGGGGCCA
  GCTGAGGGGCATCCGCCTGAAGGCCCCTGGAGGCCCAGTCTCAGCTTTTCTGGGCATCCCCTTTGCAGAG
  CCACCTGTGGGCTCACGTAGATTTATGCCACCAGAGCCCAAGCGGCCCTGGTCAGGAGTGTTGGATGCTA
  CCACCTTCCAAAATGTCTGCTACCAGTACGTGGACACCCTGTACCCTGGGTTTGAGGGTACTGAGATGTG
  GAACCCCAACCGAGAGTTGAGTGAAGACTGCCTGTATCTTAATGTGTGGACACCATACCCCAGACCTGCT
  TCTCCCACACCTGTCCTCATCTGGATCTATGGGGGTGGTTTCTACAGCGGAGCGGCCTCCTTGGATGTGT
  ATGACGGCCGTTTCCTGGCCCAGGTTGAGGGAGCTGTGTTGGTATCTATGAACTACCGAGTGGGAACCTT
  TGGCTTCTTGGCCCTACCAGGAAGCAGAGAAGCCCCTGGCAATGTAGGTCTGCTGGATCAACGGCTTGCC
  TTGCAATGGGTGCAAGAAAATATTGCAGCCTTTGGGGGCGACCCGATGTCAGTGACTCTGTTTGGGGAGA
  GTGCGGGTGCAGCCTCCGTGGGCATGCACATACTGTCCCTGCCCAGCAGGAGCCTCTTCCACAGGGCTGT
  CCTCCAGAGTGGCACACCCAATGGGCCCTGGGCCACTGTGAGTGCTGGAGAGGCCAGGCGCAGGGCCACA
  CTGCTGGCCCGCCTTGTGGGCTGTCCCCCAGGTGGCGCTGGTGGCAATGACACCGAGCTGATAGCCTGCT
  TGAGGACAAGGCCCGCTCAGGACCTGGTGGACCACGAGTGGCACGTCCTGCCTCAAGAAAGTATCTTCCG
  ATTTTCCTTCGTGCCTGTGGTAGACGGGGACTTCCTCAGTGACACACCGGAGGCTCTCATCAATACTGGA
  GATTTTCAAGACCTGCAGGTGCTGGTGGGTGTGGTGAAGGACGAGGGCTCCTACTTTCTGGTTTACGGGG
  TCCCAGGCTTCAGCAAAGACAATGAATCTCTCATCAGCCGGGCCCAGTTCCTGGCTGGGGTGCGGATCGG
  TGTACCCCAAGCAAGTGACCTGGCAGCCGAGGCTGTGGTCCTGCATTACACAGACTGGCTGCACCCTGAG
  GACCCTACTCACCTGAGAGATGCCATGAGTGCAGTGGTAGGCGACCACAACGTTGTGTGCCCTGTGGCCC
  AGCTGGCTGGGCGACTGGCTGCCCAAGGGGCCCGGGTCTATGCCTACATCTTTGAACACCGTGCCTCCAC
  ACTGACTTGGCCCCTCTGGATGGGGGTGCCCCATGGCTATGAAATCGAGTTCATCTTTGGGCTCCCCCTG
  GATCCCTCGCTGAACTACACCACGGAGGAGAGGATCTTTGCTCAGCGACTTATGAAATACTGGACCAATT
  TTGCCCGCACAGGGGACCCCAATGACCCTCGAGACTCCAAATCTCCACAGTGGCCACCGTACACCACTGC
  CGCGCAGCAATATGTGAGCCTGAACCTGAAGCCCTTAGAGGTGCGGCGGGGACTGCGCGCCCAGACCTGC
  GCCTTCTGGAATCGCTTTCTCCCCAAATTGCTCAGCGCCACCGATACTCTGGACGAGGCGGAGCGCCAGT
  GGAAGGCCGAGTTCCACCGCTGGAGCTCCTACATGGTGCACTGGAAGAACCAGTTCGACCACTATAGCAA
  GCAGGAGCGCTGCTCAGACCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR226310 representing NM_009599
  Red=Cloning site Green=Tags(s)
  
  MRPPWYPLHTPSLAFPLLFLLLSLLGGGARAEGREDPQLLVRVRGGQLRGIRLKAPGGPVSAFLGIPFAE
  PPVGSRRFMPPEPKRPWSGVLDATTFQNVCYQYVDTLYPGFEGTEMWNPNRELSEDCLYLNVWTPYPRPA
  SPTPVLIWIYGGGFYSGAASLDVYDGRFLAQVEGAVLVSMNYRVGTFGFLALPGSREAPGNVGLLDQRLA
  LQWVQENIAAFGGDPMSVTLFGESAGAASVGMHILSLPSRSLFHRAVLQSGTPNGPWATVSAGEARRRAT
  LLARLVGCPPGGAGGNDTELIACLRTRPAQDLVDHEWHVLPQESIFRFSFVPVVDGDFLSDTPEALINTG
  DFQDLQVLVGVVKDEGSYFLVYGVPGFSKDNESLISRAQFLAGVRIGVPQASDLAAEAVVLHYTDWLHPE
  DPTHLRDAMSAVVGDHNVVCPVAQLAGRLAAQGARVYAYIFEHRASTLTWPLWMGVPHGYEIEFIFGLPL
  DPSLNYTTEERIFAQRLMKYWTNFARTGDPNDPRDSKSPQWPPYTTAAQQYVSLNLKPLEVRRGLRAQTC
  AFWNRFLPKLLSATDTLDEAERQWKAEFHRWSSYMVHWKNQFDHYSKQERCSDL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009599
• ORF Size: 1842 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_009599.1, NM_009599.2, NM_009599.3, NM_009599.4, NP_033729.1
• RefSeq Size: 2184
• RefSeq ORF: 1845
• Locus ID: 11423
• MW: 68.6 kDa
• Gene Summary: Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft. [UniProtKB/Swiss-Prot Function]