Ar (NM_013476) Mouse Tagged ORF Clone

CAT#: MR226737

Ar (Myc-DDK-tagged) - Mouse androgen receptor (Ar)

Product Images

Western blot validation of overexpressed Ar protein using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with MR226737 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ar
• Synonyms: AW320017; Tfm
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR226737 representing NM_013476.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGAGGTGCAGTTAGGGCTGGGAAGGGTCTACCCACGGCCCCCATCCAAGACCTATCGAGGAGCGTTC
  CAGAATCTGTTCCAGAGCGTGCGCGAAGCGATCCAGAACCCGGGCCCCAGGCACCCTGAGGCCGCTAAC
  ATAGCACCTCCCGGCGCCTGTTTACAGCAGAGGCAGGAGACTAGCCCCCGGCGGCGGCGGCGGCAGCAG
  CACACTGAGGATGGTTCTCCTCAAGCCCACATCAGAGGCCCCACAGGCTACCTGGCCCTGGAGGAGGAA
  CAGCAGCCTTCACAGCAGCAGGCAGCCTCCGAGGGCCACCCTGAGAGCAGCTGCCTCCCCGAGCCTGGG
  GCGGCCACCGCTCCTGGCAAGGGGCTGCCGCAGCAGCCACCAGCTCCTCCAGATCAGGATGACTCAGCT
  GCCCCATCCACGTTGTCCCTGCTGGGCCCCACTTTCCCAGGCTTAAGCAGCTGCTCCGCCGACATTAAA
  GACATTTTGAACGAGGCCGGCACCATGCAACTTCTTCAGCAGCAGCAACAACAGCAGCAGCACCAACAG
  CAGCACCAACAGCACCAACAGCAGCAGGAGGTAATCTCCGAAGGCAGCAGCGCAAGAGCCAGGGAGGCC
  ACGGGGGCTCCCTCTTCCTCCAAGGATAGTTACCTAGGGGGCAATTCAACCATATCTGACAGTGCCAAG
  GAGTTGTGTAAAGCAGTGTCTGTGTCCATGGGATTGGGTGTGGAAGCATTGGAACATCTGAGTCCAGGG
  GAACAGCTTCGGGGAGACTGCATGTACGCGTCGCTCCTGGGAGGTCCACCCGCGGTGCGTCCCACTCCT
  TGTGCGCCGCTGCCCGAATGCAAAGGTCTTCCCCTGGACGAAGGCCCAGGCAAAAGCACTGAAGAGACT
  GCTGAGTATTCCTCTTTCAAGGGAGGTTACGCCAAAGGATTGGAAGGTGAGAGCTTGGGGTGCTCTGGC
  AGCAGTGAAGCAGGTAGCTCTGGGACACTTGAGATCCCGTCCTCTCTGTCTCTGTATAAATCTGGAGCA
  CTAGACGAGGCAGCAGCATACCAGAATCGCGACTACTACAACTTTCCGCTGGCTCTGTCCGGGCCGCCG
  CACCCCCCGCCCCCTACCCATCCACACGCCCGTATCAAGCTGGAGAACCCATTGGACTACGGCAGCGCC
  TGGGCTGCGGCGGCAGCGCAATGCCGCTATGGGGACTTGGGTAGTCTACATGGAGGGAGTGTAGCCGGG
  CCCAGCACTGGATCGCCCCCAGCCACCACCTCTTCTTCCTGGCATACTCTCTTCACAGCTGAAGAAGGC
  CAATTATATGGGCCAGGAGGCGGGGGCGGCAGCAGCAGCCCAAGCGATGCCGGGCCTGTAGCCCCCTAT
  GGCTACACTCGGCCCCCTCAGGGGCTGACAAGCCAGGAGAGTGACTACTCTGCCTCCGAAGTGTGGTAT
  CCTGGTGGAGTTGTGAACAGAGTACCCTATCCCAGTCCCAATTGTGTCAAAAGTGAAATGGGACCTTGG
  ATGGAGAACTACTCCGGACCTTATGGGGACATGCGTTTGGACAGTACCAGGGACCATGTTTTACCCATC
  GACTATTACTTTCCACCCCAGAAGACCTGCCTGATCTGTGGAGATGAAGCTTCTGGCTGTCACTACGGA
  GCTCTCACTTGTGGCAGCTGCAAGGTCTTCTTCAAAAGAGCCGCTGAAGGGAAACAGAAGTATCTATGT
  GCCAGCAGAAACGATTGTACCATTGATAAATTTCGGAGGAAAAATTGCCCATCTTGTCGTCTCCGGAAA
  TGTTATGAAGCAGGGATGACTCTGGGAGCTCGTAAGCTGAAGAAACTTGGAAATCTAAAACTACAGGAG
  GAAGGAGAAAACTCCAATGCTGGCAGCCCCACTGAGGACCCATCCCAGAAGATGACTGTATCACACATT
  GAAGGCTATGAATGTCAGCCTATCTTTCTTAACGTCCTGGAAGCCATTGAGCCAGGAGTGGTGTGTGCC
  GGACATGACAACAACCAACCAGATTCCTTTGCTGCCTTGTTATCTAGCCTCAATGAGCTTGGAGAGAGG
  CAGCTTGTGCATGTGGTCAAGTGGGCCAAGGCCTTGCCTGGCTTCCGCAACTTGCATGTGGATGACCAG
  ATGGCGGTCATTCAGTATTCCTGGATGGGACTGATGGTATTTGCCATGGGTTGGCGGTCCTTCACTAAT
  GTCAACTCCAGGATGCTCTACTTTGCACCTGACTTGGTTTTCAATGAGTACCGCATGCACAAGTCTCGG
  ATGTACAGCCAGTGTGTGAGGATGAGGCACCTGTCTCAAGAGTTTGGATGGCTCCAAATAACCCCCCAG
  GAATTCCTGTGCATGAAAGCACTGCTGCTCTTCAGCATTATTCCAGTGGATGGGCTGAAAAATCAAAAA
  TTCTTTGATGAACTTCGAATGAACTACATCAAGGAACTCGATCGCATCATTGCATGCAAAAGAAAGAAT
  CCCACATCCTGCTCAAGGCGCTTCTACCAGCTCACCAAGCTCCTGGATTCTGTGCAGCCTATTGCAAGA
  GAGCTGCATCAGTTCACTTTTGACCTGCTAATCAAGTCCCATATGGTGAGCGTGGACTTTCCTGAAATG
  ATGGCAGAGATCATCTCTGTGCAAGTGCCCAAGATCCTTTCTGGGAAAGTCAAGCCCATCTATTTCCAC
  ACACAG
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGAT
  ATCCTGGATTACAAGGATGACGACGATAAGGTTTAA
  
  Protein Sequence

  >Peptide sequence encoded by MR226737
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MEVQLGLGRVYPRPPSKTYRGAFQNLFQSVREAIQNPGPRHPEAANIAPPGACLQQRQETSPRRRRRQQ
  HTEDGSPQAHIRGPTGYLALEEEQQPSQQQAASEGHPESSCLPEPGAATAPGKGLPQQPPAPPDQDDSA
  APSTLSLLGPTFPGLSSCSADIKDILNEAGTMQLLQQQQQQQQHQQQHQQHQQQQEVISEGSSARAREA
  TGAPSSSKDSYLGGNSTISDSAKELCKAVSVSMGLGVEALEHLSPGEQLRGDCMYASLLGGPPAVRPTP
  CAPLPECKGLPLDEGPGKSTEETAEYSSFKGGYAKGLEGESLGCSGSSEAGSSGTLEIPSSLSLYKSGA
  LDEAAAYQNRDYYNFPLALSGPPHPPPPTHPHARIKLENPLDYGSAWAAAAAQCRYGDLGSLHGGSVAG
  PSTGSPPATTSSSWHTLFTAEEGQLYGPGGGGGSSSPSDAGPVAPYGYTRPPQGLTSQESDYSASEVWY
  PGGVVNRVPYPSPNCVKSEMGPWMENYSGPYGDMRLDSTRDHVLPIDYYFPPQKTCLICGDEASGCHYG
  ALTCGSCKVFFKRAAEGKQKYLCASRNDCTIDKFRRKNCPSCRLRKCYEAGMTLGARKLKKLGNLKLQE
  EGENSNAGSPTEDPSQKMTVSHIEGYECQPIFLNVLEAIEPGVVCAGHDNNQPDSFAALLSSLNELGER
  QLVHVVKWAKALPGFRNLHVDDQMAVIQYSWMGLMVFAMGWRSFTNVNSRMLYFAPDLVFNEYRMHKSR
  MYSQCVRMRHLSQEFGWLQITPQEFLCMKALLLFSIIPVDGLKNQKFFDELRMNYIKELDRIIACKRKN
  PTSCSRRFYQLTKLLDSVQPIARELHQFTFDLLIKSHMVSVDFPEMMAEIISVQVPKILSGKVKPIYFH
  TQ
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
  
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013476
• ORF Size: 2697 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013476.4
• RefSeq Size: 2999 bp
• RefSeq ORF: 2700 bp
• Locus ID: 11835
• UniProt ID: P19091
• Cytogenetics: X 42.82 cM
• MW: 98.2 kDa
• Gene Summary: This gene encodes a nuclear hormone receptor containing zinc finger and DNA-binding domains. The encoded protein is a key regulator of signalling by androgens, a class of steroid hormones involved in male reproductive development. The protein responds to hormone signalling by translocating to the nucleus, forming dimers, and binding to androgen response elements (AREs) in the promoters of target genes, which are subsequently transcriptionally activated. Activity of this protein is negatively regulated by nuclear receptor subfamily 0 group B member 1 (Nr0b1, also known as Dax1). Mutations in this gene result in feminized genitals and infertility in male animals. Loss of function in female animals also causes problems in reproductive development and function. [provided by RefSeq, May 2015]