Runx2 (NM_001271630) Mouse Tagged ORF Clone

CAT#: MR230001

Runx2 (myc-DDK-tagged) - Mouse runt related transcription factor 2 (Runx2), transcript variant 5

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Runx2
• Synonyms: AML3; Cbf; Cbfa-1; Cbfa1; LS3; Osf2; Pebp2a1; PEBP2aA; Pebpa2a
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR230001 representing NM_001271630
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGTATTCCTGTAGATCCGAGCACCAGCCGGCGCTTCAGCCCCCCCTCCAGCAGCCTGCAGCCCGGCA
  AGATGAGCGACGTGAGCCCGGTGGTGGCTGCGCAGCAGCAGCAACAGCAGCAGCAGCAGCAGCAACAGCA
  GCAGCAACAACAGCAACAGCAACAACAGCAGCAGCAGCAGCAGCAGCAGGAGGCGGCCGCAGCAGCAGCG
  GCGGCAGCGGCGGCGGCAGCAGCGGCGGCGGCCGCAGTGCCCCGATTGAGGCCGCCGCACGACAACCGCA
  CCATGGTGGAGATCATCGCGGACCACCCGGCCGAACTGGTCCGCACCGACAGTCCCAACTTCCTGTGCTC
  CGTGCTGCCCTCGCACTGGCGGTGCAACAAGACCCTGCCCGTGGCCTTCAAGGTTGTAGCCCTCGGAGAG
  GTACCAGATGGGACTGTGGTTACCGTCATGGCCGGGAATGATGAGAACTACTCCGCCGAGCTCCGAAATG
  CCTCCGCTGTTATGAAAAACCAAGTAGCCAGGTTCAACGATCTGAGATTTGTGGGCCGGAGCGGACGAGG
  CAAGAGTTTCACCTTGACCATAACAGTCTTCACAAATCCTCCCCAAGTGGCCACTTACCACAGAGCTATT
  AAAGTGACAGTGGACGGTCCCCGGGAACCAAGAAATCCCAGGCAGGCACAGTCTTCCCCACCGTGGTCCT
  ATGACCAGTCTTACCCCTCCTATCTGAGCCAGATGACATCCCCATCCATCCACTCCACCACGCCGCTGTC
  TTCCACACGGGGCACCGGGCTACCTGCCATCACTGACGTGCCCAGGCGTATTTCAGATGATGACACTGCC
  ACCTCTGACTTCTGCCTCTGGCCTTCCTCTCTCAGTAAGAAGAGCCAGGCAGGTGCTTCAGAACTGGGCC
  CTTTTTCAGACCCCAGGCAGTTCCCAAGCATTTCATCCCTCACTGAGAGCCGCTTCTCCAACCCACGAAT
  GCACTACCCAGCCACCTTTACCTACACCCCGCCAGTCACGTCAGGCATGTCCCTCGGCATGTCCGCCACC
  ACTCACTACCACACGTACCTGCCACCACCCTACCCCGGCTCTTCCCAAAGCCAGAGTGGACCCTTCCAGA
  CCAGCAGCACTCCATATCTCTACTATGGTACTTCGTCAGCATCCTATCAGTTCCCAATGGTACCCGGGGG
  AGACCGGTCTCCTTCCAGGATGGTCCCACCATGCACCACCACCTCGAATGGCAGCACGCTATTAAATCCA
  AATTTGCCTAACCAGAATGATGGTGTTGACGCTGACGGAAGCCACAGCAGTTCCCCAACTGTTTTGAATT
  CTAGCGGCAGAATGGATGAGTCTGTTTGGCGGCCATAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR230001 representing NM_001271630
  Red=Cloning site Green=Tags(s)
  
  MRIPVDPSTSRRFSPPSSSLQPGKMSDVSPVVAAQQQQQQQQQQQQQQQQQQQQQQQQQQQQQEAAAAAA
  AAAAAAAAAAAAVPRLRPPHDNRTMVEIIADHPAELVRTDSPNFLCSVLPSHWRCNKTLPVAFKVVALGE
  VPDGTVVTVMAGNDENYSAELRNASAVMKNQVARFNDLRFVGRSGRGKSFTLTITVFTNPPQVATYHRAI
  KVTVDGPREPRNPRQAQSSPPWSYDQSYPSYLSQMTSPSIHSTTPLSSTRGTGLPAITDVPRRISDDDTA
  TSDFCLWPSSLSKKSQAGASELGPFSDPRQFPSISSLTESRFSNPRMHYPATFTYTPPVTSGMSLGMSAT
  THYHTYLPPPYPGSSQSQSGPFQTSSTPYLYYGTSSASYQFPMVPGGDRSPSRMVPPCTTTSNGSTLLNP
  NLPNQNDGVDADGSHSSSPTVLNSSGRMDESVWRPY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001271630
• ORF Size: 1368 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001271630.1, NP_001258559.1
• RefSeq Size: 5829
• RefSeq ORF: 1371
• Locus ID: 12393
• MW: 49.9 kDa
• Gene Summary: This gene encodes a member of the runt domain-containing family of transcription factors. This protein is essential for osteoblastic differentiation and skeletal morphogenesis and acts as a scaffold for nucleic acids and regulatory factors involved in skeletal gene expression. The protein can bind DNA both as a monomer or, with more affinity, as a subunit of a heterodimeric complex. Transcript variants that encode different protein isoforms result from the use of alternate promoters as well as alternate splicing. [provided by RefSeq, Sep 2015]