Kif5a (NM_001039000) Mouse Tagged ORF Clone

CAT#: MR231496

Kif5a (myc-DDK-tagged) - Mouse kinesin family member 5A (Kif5a), transcript variant 1

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Kif5a
• Synonyms: D10Bwg0738e; Khc; Kif5; Kns; mKIAA4086
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR231496 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAGACTAACAACGAATGCAGCATCAAGGTGCTTTGCCGATTTCGGCCCCTGAACCAGGCCGAGA
  TTCTGCGGGGGGACAAGTTCATCCCCATTTTCCAAGGGGACGACAGCGTCATTATTGGGGGAAAGCCATA
  TGTCTTTGACCGCGTCTTCCCCCCAAACACCACTCAGGAGCAGGTTTACCACGCCTGTGCCATGCAGATC
  GTCAAAGACGTCCTTGCTGGTTACAATGGCACAATCTTCGCTTATGGACAGACATCCTCAGGGAAAACGC
  ATACCATGGAGGGGAAGCTGCACGACCCTCAGCTGATGGGCATCATTCCCCGGATCGCTCGAGACATCTT
  CAACCACATCTACTCCATGGATGAGAACCTTGAATTCCACATTAAGGTATCTTACTTCGAGATTTACCTG
  GATAAGATCCGTGACCTTTTGGATGTGACCAAGACGAACCTGTCCGTGCATGAGGACAAAAACCGGGTGC
  CGTTTGTCAAGGGTTGTACCGAACGCTTTGTGTCCAGCCCAGAGGAGATTCTGGATGTGATCGATGAGGG
  GAAGTCCAACCGTCACGTAGCTGTCACCAACATGAACGAGCACAGTTCTCGGAGCCACAGCATCTTCCTC
  ATCAACATCAAGCAGGAGAACGTAGAGACCGAGCAGAAGCTCAGCGGGAAGCTGTACCTCGTGGATCTGG
  CCGGAAGCGAGAAGGTCAGCAAGACAGGGGCAGAGGGAGCCGTTCTGGACGAGGCAAAGAATATCAACAA
  GTCGCTGTCGGCCCTGGGGAACGTGATCTCTGCACTGGCAGAGGGCACCAAAAGCTACGTGCCGTACCGC
  GACAGCAAAATGACGAGGATTCTCCAGGACTCTCTGGGAGGGAACTGCAGGACTACCATGTTCATCTGCT
  GCTCGCCGTCCAGCTACAATGACGCAGAGACCAAGTCCACGCTCATGTTTGGACAGCGGGCGAAGACCAT
  CAAGAACACTGCCTCAGTGAATCTGGAGCTGACTGCTGAGCAGTGGAAGAAGAAGTATGAGAAGGAGAAG
  GAGAAGACCAAGGCCCAGAAGGAGACAATTGCGAAGCTAGAGGCTGAGCTTAGCCGGTGGCGCAATGGAG
  AGAATGTGCCTGAGACTGAGCGCCTGGCTGGAGAGGACTCAGCTCTGGGAGCTGAGCTCTGCGAGGAGAC
  CCCTGTGAATGACAACTCATCCATTGTGGTACGCATCGCACCTGAGGAAAGGCAGAAATATGAGGAAGAG
  ATCCGCCGTCTCTACAAGCAGCTTGATGACAAGGATGATGAGATCAACCAGCAGAGCCAGCTCATTGAGA
  AGCTGAAGCAGCAGATGCTGGACCAGGAAGAGCTGCTCGTGTCCACTCGGGGAGACAACGAGAAGGTCCA
  GCGGGAGCTTAGCCACCTGCAGTCCGAGAACGATGCTGCGAAGGACGAGGTGAAGGAAGTGCTGCAGGCC
  CTAGAGGAGCTGGCGGTCAACTACGACCAGAAGTCCCAGGAGGTGGAGGAGAAGAGCCAGCAGAACCAGC
  TGCTGGTGGACGAGCTGTCCCAGAAAGTGGCCACCATGCTGTCCCTGGAGTCCGAGCTACAGCGGCTCCA
  GGAGGTCAGTGGACACCAGCGAAAGCGGATCGCTGAGGTGCTGAATGGGCTGATGAGGGACCTGAGTGAG
  TTCAGTGTCATCGTGGGCAACGGCGAGATTAAGCTGCCCGTGGAGATCAGTGGGGCCATCGAGGAGGAGT
  TCACGGTGGCCCGGCTCTACATCAGCAAGATCAAGTCGGAGGTGAAGTCCGTGGTTAAGCGATGTCGGCA
  GCTGGAGAACCTCCAGGTGGAGTGTCATCGCAAGATGGAGGTGACCGGTAGGGAGCTGTCATCTTGCCAA
  CTGCTCATCTCACAGCATGAGGCCAAGATCCGTTCACTCACGGAGTACATGCAGACTGTGGAGTTGAAGA
  AACGGCACCTGGAAGAGTCCTACGACTCCCTGAGCGATGAGCTTGCCAGGCTCCAGGCGCACGAAACTGT
  ACACGAGGTAGCTCTGAAAGACAAGGAGCCAGACACACAGGACGCGGAGGAGGTGAAGAAGGCCCTGGAA
  CTACAGATGGAGAATCACCGTGAGGCCCATCACCGGCAGCTGGCCCGCCTCCGGGATGAGATTAATGAGA
  AACAGAAAACCATTGATGAGCTGAAAGACCTGAACCAGAAGCTCCAGTTAGAGCTGGAGAAGCTTCAGGC
  CGACTATGAGAGGCTGAAGAATGAAGAGAACGAGAAGAGCGCCAAGCTCCAGGAGCTGACATTTCTGTAT
  GAGCGACATGAGCAGTCCAAGCAGGACCTCAAGGGGCTGGAGGAGACAGTTGCCCGTGAACTCCAGACCC
  TCCACAACCTTCGCAAGCTGTTCGTTCAAGACGTCACGACTCGAGTCAAGAAAAGTGCAGAAATGGAGCC
  CGAGGACAGTGGGGGGATTCATTCCCAAAAGCAGAAGATCTCCTTTCTTGAGAACAACCTGGAACAGCTT
  ACAAAGGTTCACAAACAGCTGGTACGTGACAATGCAGATCTGCGTTGTGAGCTTCCTAAATTGGAAAAAC
  GACTTCGGGCTACGGCTGAGAGAGTTAAGGCCCTGGAGGGTGCACTGAAGGAGGCCAAGGAGGGCGCTAT
  GAAGGACAAGCGTAGATACCAGCAGGAGGTGGACCGCATCAAAGAAGCCGTGCGGTACAAGAGCTCCGGC
  AAGCGGGGCCATTCTGCCCAGATCGCTAAGCCTGTGAGGCCTGGCCACTATCCTGCCTCCTCACCCACCA
  ACCCCTACGGCACCCGGAGCCCCGAGTGTATCAGCTACACCAACAACCTCTTCCAGAACTACCAGAACCT
  GCACCTGCAGGCTGCGCCTAGCTCCACTTCAGATATGTACTTTGCCAGCAGCGGAGCCACATCTGTTGCC
  CCCTTGGCTTCCTACCAGAAGGCCAACATGGACAATGGAAATGCCACAGATATCAACGACAACAGGAGTG
  ACCTGCCGTGTGGCTATGAGGCTGAGGACCAGGCCAAGCTTTTCCCTCTCCACCAAGAGACAGCAGCCAG
  C
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR231496 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAETNNECSIKVLCRFRPLNQAEILRGDKFIPIFQGDDSVIIGGKPYVFDRVFPPNTTQEQVYHACAMQI
  VKDVLAGYNGTIFAYGQTSSGKTHTMEGKLHDPQLMGIIPRIARDIFNHIYSMDENLEFHIKVSYFEIYL
  DKIRDLLDVTKTNLSVHEDKNRVPFVKGCTERFVSSPEEILDVIDEGKSNRHVAVTNMNEHSSRSHSIFL
  INIKQENVETEQKLSGKLYLVDLAGSEKVSKTGAEGAVLDEAKNINKSLSALGNVISALAEGTKSYVPYR
  DSKMTRILQDSLGGNCRTTMFICCSPSSYNDAETKSTLMFGQRAKTIKNTASVNLELTAEQWKKKYEKEK
  EKTKAQKETIAKLEAELSRWRNGENVPETERLAGEDSALGAELCEETPVNDNSSIVVRIAPEERQKYEEE
  IRRLYKQLDDKDDEINQQSQLIEKLKQQMLDQEELLVSTRGDNEKVQRELSHLQSENDAAKDEVKEVLQA
  LEELAVNYDQKSQEVEEKSQQNQLLVDELSQKVATMLSLESELQRLQEVSGHQRKRIAEVLNGLMRDLSE
  FSVIVGNGEIKLPVEISGAIEEEFTVARLYISKIKSEVKSVVKRCRQLENLQVECHRKMEVTGRELSSCQ
  LLISQHEAKIRSLTEYMQTVELKKRHLEESYDSLSDELARLQAHETVHEVALKDKEPDTQDAEEVKKALE
  LQMENHREAHHRQLARLRDEINEKQKTIDELKDLNQKLQLELEKLQADYERLKNEENEKSAKLQELTFLY
  ERHEQSKQDLKGLEETVARELQTLHNLRKLFVQDVTTRVKKSAEMEPEDSGGIHSQKQKISFLENNLEQL
  TKVHKQLVRDNADLRCELPKLEKRLRATAERVKALEGALKEAKEGAMKDKRRYQQEVDRIKEAVRYKSSG
  KRGHSAQIAKPVRPGHYPASSPTNPYGTRSPECISYTNNLFQNYQNLHLQAAPSSTSDMYFASSGATSVA
  PLASYQKANMDNGNATDINDNRSDLPCGYEAEDQAKLFPLHQETAAS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001039000
• ORF Size: 3084 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001039000.1, NM_001039000.2, NM_001039000.3, NM_001039000.4, NP_001034089.1
• RefSeq Size: 6334 bp
• RefSeq ORF: 3084 bp
• Locus ID: 16572
• Cytogenetics: 10 74.5 cM
• MW: 117 kDa