Kif5a (NM_001039000) Mouse Untagged Clone

CAT#: MC229285

Kif5a (untagged) - Mouse kinesin family member 5A (Kif5a), transcript variant 1

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Kif5a
• Synonyms: D10Bwg0738e; Khc; Kif5; Kns; mKIAA4086
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC229285 representing NM_001039000
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAGACTAACAACGAATGCAGCATCAAGGTGCTTTGCCGATTTCGGCCCCTGAACCAGGCCGAGA
  TTCTGCGGGGGGACAAGTTCATCCCCATTTTCCAAGGGGACGACAGCGTCATTATTGGGGGAAAGCCATA
  TGTCTTTGACCGCGTCTTCCCCCCAAACACCACTCAGGAGCAGGTTTACCACGCCTGTGCCATGCAGATC
  GTCAAAGACGTCCTTGCTGGTTACAATGGCACAATCTTCGCTTATGGACAGACATCCTCAGGGAAAACGC
  ATACCATGGAGGGGAAGCTGCACGACCCTCAGCTGATGGGCATCATTCCCCGGATCGCTCGAGACATCTT
  CAACCACATCTACTCCATGGATGAGAACCTTGAATTCCACATTAAGGTATCTTACTTCGAGATTTACCTG
  GATAAGATCCGTGACCTTTTGGATGTGACCAAGACGAACCTGTCCGTGCATGAGGACAAAAACCGGGTGC
  CGTTTGTCAAGGGTTGTACCGAACGCTTTGTGTCCAGCCCAGAGGAGATTCTGGATGTGATCGATGAGGG
  GAAGTCCAACCGTCACGTAGCTGTCACCAACATGAACGAGCACAGTTCTCGGAGCCACAGCATCTTCCTC
  ATCAACATCAAGCAGGAGAACGTAGAGACCGAGCAGAAGCTCAGCGGGAAGCTGTACCTCGTGGATCTGG
  CCGGAAGCGAGAAGGTCAGCAAGACAGGGGCAGAGGGAGCCGTTCTGGACGAGGCAAAGAATATCAACAA
  GTCGCTGTCGGCCCTGGGGAACGTGATCTCTGCACTGGCAGAGGGCACCAAAAGCTACGTGCCGTACCGC
  GACAGCAAAATGACGAGGATTCTCCAGGACTCTCTGGGAGGGAACTGCAGGACTACCATGTTCATCTGCT
  GCTCGCCGTCCAGCTACAATGACGCAGAGACCAAGTCCACGCTCATGTTTGGACAGCGGGCGAAGACCAT
  CAAGAACACTGCCTCAGTGAATCTGGAGCTGACTGCTGAGCAGTGGAAGAAGAAGTATGAGAAGGAGAAG
  GAGAAGACCAAGGCCCAGAAGGAGACAATTGCGAAGCTAGAGGCTGAGCTTAGCCGGTGGCGCAATGGAG
  AGAATGTGCCTGAGACTGAGCGCCTGGCTGGAGAGGACTCAGCTCTGGGAGCTGAGCTCTGCGAGGAGAC
  CCCTGTGAATGACAACTCATCCATTGTGGTACGCATCGCACCTGAGGAAAGGCAGAAATATGAGGAAGAG
  ATCCGCCGTCTCTACAAGCAGCTTGATGACAAGGATGATGAGATCAACCAGCAGAGCCAGCTCATTGAGA
  AGCTGAAGCAGCAGATGCTGGACCAGGAAGAGCTGCTCGTGTCCACTCGGGGAGACAACGAGAAGGTCCA
  GCGGGAGCTTAGCCACCTGCAGTCCGAGAACGATGCTGCGAAGGACGAGGTGAAGGAAGTGCTGCAGGCC
  CTAGAGGAGCTGGCGGTCAACTACGACCAGAAGTCCCAGGAGGTGGAGGAGAAGAGCCAGCAGAACCAGC
  TGCTGGTGGACGAGCTGTCCCAGAAAGTGGCCACCATGCTGTCCCTGGAGTCCGAGCTACAGCGGCTCCA
  GGAGGTCAGTGGACACCAGCGAAAGCGGATCGCTGAGGTGCTGAATGGGCTGATGAGGGACCTGAGTGAG
  TTCAGTGTCATCGTGGGCAACGGCGAGATTAAGCTGCCCGTGGAGATCAGTGGGGCCATCGAGGAGGAGT
  TCACGGTGGCCCGGCTCTACATCAGCAAGATCAAGTCGGAGGTGAAGTCCGTGGTTAAGCGATGTCGGCA
  GCTGGAGAACCTCCAGGTGGAGTGTCATCGCAAGATGGAGGTGACCGGTAGGGAGCTGTCATCTTGCCAA
  CTGCTCATCTCACAGCATGAGGCCAAGATCCGTTCACTCACGGAGTACATGCAGACTGTGGAGTTGAAGA
  AACGGCACCTGGAAGAGTCCTACGACTCCCTGAGCGATGAGCTTGCCAGGCTCCAGGCGCACGAAACTGT
  ACACGAGGTAGCTCTGAAAGACAAGGAGCCAGACACACAGGACGCGGAGGAGGTGAAGAAGGCCCTGGAA
  CTACAGATGGAGAATCACCGTGAGGCCCATCACCGGCAGCTGGCCCGCCTCCGGGATGAGATTAATGAGA
  AACAGAAAACCATTGATGAGCTGAAAGACCTGAACCAGAAGCTCCAGTTAGAGCTGGAGAAGCTTCAGGC
  CGACTATGAGAGGCTGAAGAATGAAGAGAACGAGAAGAGCGCCAAGCTCCAGGAGCTGACATTTCTGTAT
  GAGCGACATGAGCAGTCCAAGCAGGACCTCAAGGGGCTGGAGGAGACAGTTGCCCGTGAACTCCAGACCC
  TCCACAACCTTCGCAAGCTGTTCGTTCAAGACGTCACGACTCGAGTCAAGAAAAGTGCAGAAATGGAGCC
  CGAGGACAGTGGGGGGATTCATTCCCAAAAGCAGAAGATCTCCTTTCTTGAGAACAACCTGGAACAGCTT
  ACAAAGGTTCACAAACAGCTGGTACGTGACAATGCAGATCTGCGTTGTGAGCTTCCTAAATTGGAAAAAC
  GACTTCGGGCTACGGCTGAGAGAGTTAAGGCCCTGGAGGGTGCACTGAAGGAGGCCAAGGAGGGCGCTAT
  GAAGGACAAGCGTAGATACCAGCAGGAGGTGGACCGCATCAAAGAAGCCGTGCGGTACAAGAGCTCCGGC
  AAGCGGGGCCATTCTGCCCAGATCGCTAAGCCTGTGAGGCCTGGCCACTATCCTGCCTCCTCACCCACCA
  ACCCCTACGGCACCCGGAGCCCCGAGTGTATCAGCTACACCAACAACCTCTTCCAGAACTACCAGAACCT
  GCACCTGCAGGCTGCGCCTAGCTCCACTTCAGATATGTACTTTGCCAGCAGCGGAGCCACATCTGTTGCC
  CCCTTGGCTTCCTACCAGAAGGCCAACATGGACAATGGAAATGCCACAGATATCAACGACAACAGGAGTG
  ACCTGCCGTGTGGCTATGAGGCTGAGGACCAGGCCAAGCTTTTCCCTCTCCACCAAGAGACAGCAGCCAG
  CTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001039000
• Insert Size: 3084 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This clone expresses the complete ORF with c-terminal tags of Myc-DDK.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001039000.4, NP_001034089.1
• RefSeq Size: 6334 bp
• RefSeq ORF: 3084 bp
• Locus ID: 16572
• Cytogenetics: 10 74.5 cM