Kcnt1 (NM_001302351) Mouse Tagged ORF Clone

CAT#: MR231697

Kcnt1 (myc-DDK-tagged) - Mouse potassium channel, subfamily T, member 1 (Kcnt1), transcript variant 3

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Kcnt1
• Synonyms: C030030G16Rik; Slack; slo2; Slo2.2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR231697 representing NM_001302351
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCGGGCCAAGCTGCCGCGCTCGCCGTCCGAGGGCAAGGCGGGTCCGGGGGACACCCCAGCCGGCG
  CTGCAGCCCCTGAGGAGCCACACGGGCTCAGCCCGCTACTGCCGGCCCGCGGCGGGGGCTCCGTGGGCAG
  CGACGTGGGCCAGAGGGTCCAGGTGGAGTTCTATGTCAATGAGAACACCTTCAAAGAGCGGCTCAAGCTG
  TTCTTCATCAAAAACCAAAGATCCAGCCTGAGGATCCGGCTGTTCAACTTCTCCCTCAAGCTCCTCACCT
  GCCTGCTGTATATCGTCCGTGTCCTGCTTGACAACCCAGACCAGGGCATCGGATGCTGGGGCTGCACGAA
  GTATAACTACACTTTCAATGGCTCATCCTCTGAGTTCCACTGGGCTCCCATCCTGTGGGTGGAGAGGAAA
  ATGGCTCTGTGGGTGATCCAGGTCATTGTGGCCACAATAAGCTTCTTAGAGACCATGCTCATCATTTACC
  TCAGCTACAAAGGCAACATCTGGGAGCAGATATTCCATGTGTCTTTCGTCTTGGAGATGATCAACACACT
  GCCCTTCATCATCACGGTCTTCTGGCCACCTCTGCGGAACCTGTTCATCCCCGTGTTTCTCAACTGCTGG
  CTGGCCAAGCATGCGCTGGAGAACATGATTAATGACTTCCACCGTGCCATCCTACGCACACAGTCAGCCA
  TGTTCAACCAGGTGCTCATCCTGTTCTGCACCCTGCTGTGCCTGGTCTTCACAGGGACCTGTGGGATCCA
  GCACCTGGAGCGAGCAGGTGGCAACTTGAACCTGCTGACCTCCTTCTACTTCTGCATCGTGACTTTCTCA
  ACTGTGGGCTTCGGTGATGTGACGCCCAAGATCTGGCCATCCCAGCTCCTGGTGGTCATCCTGATCTGTG
  TCACCCTTGTGGTGCTCCCACTGCAATTTGAAGAGCTTGTCTACCTCTGGATGGAGCGTCAGAAGTCAGG
  GGGCAACTATAGCCGCCACCGAGCACGGACGGAGAAGCACGTAGTCCTGTGTGTGAGCTCCCTCAAGATT
  GATCTCCTCATGGACTTCCTGAATGAGTTCTATGCCCATCCCCGGCTCCAGGACTACTACGTGGTCATCC
  TGTGTCCCTCTGAAATGGACGTCCAGGTGCGCAGGGTGCTGCAGATTCCCCTGTGGTCCCAGCGGGTCAT
  CTACCTCCAGGGCTCTGCCCTCAAGGACCAGGATCTCATGCGAGCCAAGATGGACAACGGAGAGGCCTGC
  TTTATCCTCAGCAGCAGGAATGAGGTGGACCGCACAGCTGCGGATCACCAGACCATCCTTCGAGCCTGGG
  CTGTGAAGGACTTTGCCCCCAACTGTCCCCTCTATGTCCAGATCCTCAAGCCCGAAAACAAGTTTCACGT
  CAAATTTGCTGACCACGTGGTATGCGAGGAAGAGTGCAAGTACGCCATGCTGGCCCTGAACTGCATCTGC
  CCGGCCACCTCCACCCTCATCACCCTGCTGGTGCACACGTCCCGTGGCCAGGAAGGACAGGAGTCTCCCG
  AGCAGTGGCAGCGCACGTACGGGAGGTGCTCGGGCAACGAGGTGTACCACATTCGCATGGGTGACAGTAA
  ATTCTTCCGGGAGTATGAGGGCAAGAGCTTCACCTACGCAGCCTTCCACGCGCACAAGAAATATGGGGTG
  TGCCTCATCGGGCTGAAGCGTGAGGAGAACAAGAGTATCCTGCTGAACCCAGGACCACGGCACATCCTGG
  CTGCCTCCGACACCTGCTTCTATATCAATATTACCAAGGAGGAGAACTCAGCTTTCATCTTCAAACAGGA
  GGAGAAGCAGAAGAGACGGGGCCTTGCAGGGCAGGCACTATATGAAGGGCCCTCCCGGCTCCCAGTGCAT
  AGCATCATCGCCTCTATGGGGACAGTGGCCATGGACCTGCAGAACACAGATTGCCGGCCCTCCCAGGGTG
  GCAGTGGCGGGGACGGCACAAAGCTGACTCTGCCCACCGAGAACGGCTCTGGTAGTCGACGCCCCAGCAT
  CGCACCCGTTCTGGAGTTGGCAGACAGCTCAGCCCTGTTGCCCTGCGACCTGCTGAGTGACCAGTCAGAG
  GATGAGGTGACACCCTCGGACGACGAGGGGCTCTCTGTGGTTGAGTACGTGAAGGGCTATCCCCCCAACT
  CACCCTACATTGGCAGCTCCCCGACTTTATGCCACCTCCTGCCTGTGAAAGCCCCCTTCTGCTGCCTGCG
  GTTGGACAAGGGCTGCAAACACAACAGCTACGAGGATGCCAAGGCCTATGGGTTCAAGAACAAGCTGATT
  ATTGTCTCTGCTGAGACGGCAGGCAACGGGCTCTACAACTTCATCGTGCCTCTGCGCGCCTACTACCGGT
  CCCGCCGGGAGCTCAACCCTATCGTGTTGCTGCTTGACAACAAGCCTGACCACCACTTCCTGGAGGCCAT
  CTGTTGCTTCCCCATGGTCTACTACATGGAGGGATCCGTGGACAACCTGGACAGCTTACTGCAGTGTGGC
  ATCATCTATGCTGACAACCTGGTGGTGGTGGACAAGGAGAGCACTATGAGCGCTGAAGAGGACTACATGG
  CAGATGCCAAGACCATCGTCAATGTGCAAACCATGTTCCGGCTTTTCCCCAGTCTCAGCATCACCACGGA
  GCTCACACACCCTTCCAACATGCGGTTCATGCAGTTCCGTGCCAAGGACAGCTACTCTCTGGCTCTTTCC
  AAACTTGAAAAGCAAGAACGGGAGAACGGCTCCAACCTGGCCTTCATGTTCCGCCTGCCATTTGCTGCTG
  GTCGAGTATTTAGTATCAGCATGTTGGATACACTGCTCTACCAGTCCTTCGTGAAGGACTACATGATCAC
  CATCACCAGGCTGCTCTTGGGCCTTGATACTACACCAGGCTCCGGCTACCTCTGTGCAATGAAGGTAACC
  GAGGACGACCTGTGGATCCGCACTTACGGCCGCCTCTTCCAGAAACTCTGCTCCTCCAGCGCCGAGATCC
  CCATCGGCATCTACAGGACCGAGTGCCATGTCTTCTCGGAGCCCCATGACGTCAGAGCCCAGTCTCAGAT
  CTCGGTGAACATGGAGGACTGCGAGGATACTCGGGAGGCCAAGGGACCCTGGGGCACACGAGCTGCATCT
  GGCAGTGGCAGCACCCATGGCCGTCACGGGGGCAGTGCTGACCCAGTGGAGCACCCACTACTACGTCGCA
  AGAGCCTGCAGTGGGCCCGCAAGCTGAGTCGCAAGAGCACCAAGCAGGCAGGGAAGGCACCTGTGGCCAC
  AGACTGGATCACCCAGCAGCGGCTCAGCCTGTACCGACGCTCAGAGCGCCAGGAGCTCTCAGAGCTGGTC
  AAGAACCGAATGAAGCACCTGGGACTGCCCACCACTGGCTATGAGGACGTAGCAAATTTAACAGCCAGTG
  ATGTGATGAATCGGGTAAACCTGGGATATTTGCAAGATGAGATGAACGATCATCACCAGAACACCCTTTC
  CTATGTACTCATCAACCCCCCGCCAGACACAAGACTGGAACCCAACGACATTGTGTACCTCATCCGTTCC
  GACCCCCTGGCCCATGTGGCCAGCAGCTCCCAGAGTCGGAAAAGCAGCTGCAGCAACAAGCTCTCATCCT
  GTAATCCTGAGACCAGGGATGAGACCCAGCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR231697 representing NM_001302351
  Red=Cloning site Green=Tags(s)
  
  MARAKLPRSPSEGKAGPGDTPAGAAAPEEPHGLSPLLPARGGGSVGSDVGQRVQVEFYVNENTFKERLKL
  FFIKNQRSSLRIRLFNFSLKLLTCLLYIVRVLLDNPDQGIGCWGCTKYNYTFNGSSSEFHWAPILWVERK
  MALWVIQVIVATISFLETMLIIYLSYKGNIWEQIFHVSFVLEMINTLPFIITVFWPPLRNLFIPVFLNCW
  LAKHALENMINDFHRAILRTQSAMFNQVLILFCTLLCLVFTGTCGIQHLERAGGNLNLLTSFYFCIVTFS
  TVGFGDVTPKIWPSQLLVVILICVTLVVLPLQFEELVYLWMERQKSGGNYSRHRARTEKHVVLCVSSLKI
  DLLMDFLNEFYAHPRLQDYYVVILCPSEMDVQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEAC
  FILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFADHVVCEEECKYAMLALNCIC
  PATSTLITLLVHTSRGQEGQESPEQWQRTYGRCSGNEVYHIRMGDSKFFREYEGKSFTYAAFHAHKKYGV
  CLIGLKREENKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRRGLAGQALYEGPSRLPVH
  SIIASMGTVAMDLQNTDCRPSQGGSGGDGTKLTLPTENGSGSRRPSIAPVLELADSSALLPCDLLSDQSE
  DEVTPSDDEGLSVVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLI
  IVSAETAGNGLYNFIVPLRAYYRSRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLQCG
  IIYADNLVVVDKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALS
  KLEKQERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMITITRLLLGLDTTPGSGYLCAMKVT
  EDDLWIRTYGRLFQKLCSSSAEIPIGIYRTECHVFSEPHDVRAQSQISVNMEDCEDTREAKGPWGTRAAS
  GSGSTHGRHGGSADPVEHPLLRRKSLQWARKLSRKSTKQAGKAPVATDWITQQRLSLYRRSERQELSELV
  KNRMKHLGLPTTGYEDVANLTASDVMNRVNLGYLQDEMNDHHQNTLSYVLINPPPDTRLEPNDIVYLIRS
  DPLAHVASSSQSRKSSCSNKLSSCNPETRDETQL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001302351
• ORF Size: 3672 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001302351.1, NP_001289280.1
• RefSeq Size: 5945
• RefSeq ORF: 3675
• Locus ID: 227632
• MW: 138.6 kDa
• Gene Summary: This gene encodes a member of the Slo potassium channel family that has shown to be activated by both sodium and chloride ions. This channel represents the largest potassium channel subunit yet identified. This channel may be important in development and pain signaling. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]