Nucleostemin (GNL3) (NM_014366) Human Tagged ORF Clone

CAT#: RC200066

GNL3 (Myc-DDK-tagged)-Human guanine nucleotide binding protein-like 3 (nucleolar) (GNL3), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY415330) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200066 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GNL3
• Synonyms: C77032; E2IG3; NNP47; NS
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200066 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAAAGGCCTAAGTTAAAGAAAGCAAGTAAACGCATGACCTGCCATAAGCGGTATAAAATCCAAAAAA
  AGGTTCGAGAACATCATCGAAAATTAAGAAAGGAGGCTAAAAAGCAGGGTCACAAGAAGCCTAGGAAAGA
  CCCAGGAGTTCCAAACAGTGCTCCCTTTAAGGAGGCTCTTCTTAGGGAAGCTGAGCTAAGGAAACAGAGG
  CTTGAAGAACTAAAACAGCAGCAGAAACTTGACAGGCAGAAGGAACTAGAAAAGAAAAGAAAACTTGAAA
  CTAATCCTGATATTAAGCCATCAAATGTGGAACCTATGGAAAAGGAGTTTGGGCTTTGCAAAACTGAGAA
  CAAAGCCAAGTCGGGCAAACAGAATTCAAAGAAGCTGTACTGCCAAGAACTTAAAAAGGTGATTGAAGCC
  TCCGATGTTGTCCTAGAGGTGTTGGATGCCAGAGATCCTCTTGGTTGCAGATGTCCTCAGGTAGAAGAGG
  CCATTGTCCAGAGTGGACAGAAAAAGCTGGTACTTATATTAAATAAATCAGATCTGGTACCAAAGGAGAA
  TTTGGAGAGCTGGCTAAATTATTTGAAGAAAGAATTGCCAACAGTGGTGTTCAGAGCCTCAACAAAACCA
  AAGGATAAAGGGAAGATAACCAAGCGTGTGAAGGCAAAGAAGAATGCTGCTCCATTCAGAAGTGAAGTCT
  GCTTTGGGAAAGAGGGCCTTTGGAAACTTCTTGGAGGTTTTCAGGAAACTTGCAGCAAAGCCATTCGGGT
  TGGAGTAATTGGTTTCCCAAATGTGGGGAAAAGCAGCATTATCAATAGCTTAAAACAAGAACAGATGTGT
  AATGTTGGTGTATCCATGGGGCTTACAAGGAGCATGCAAGTTGTCCCCTTGGACAAACAGATCACAATCA
  TAGATAGTCCGAGCTTCATCGTATCTCCACTTAATTCCTCCTCTGCGCTTGCTCTGCGAAGTCCAGCAAG
  TATTGAAGTAGTAAAACCGATGGAGGCTGCCAGTGCCATCCTTTCCCAGGCTGATGCTCGACAGGTAGTA
  CTGAAATATACTGTCCCAGGCTACAGGAATTCTCTGGAATTTTTTACTATGCTTGCTCAGAGAAGAGGTA
  TGCACCAAAAAGGTGGAATCCCAAATGTTGAAGGTGCTGCCAAACTGCTGTGGTCTGAGTGGACAGGTGC
  CTCATTAGCTTACTATTGCCATCCCCCTACATCTTGGACTCCTCCTCCATATTTTAATGAGAGTATTGTG
  GTAGACATGAAAAGCGGCTTCAATCTGGAAGAACTGGAAAAGAACAATGCACAGAGCATAAGAGCCATCA
  AGGGCCCTCATTTGGCCAATAGCATCCTTTTCCAGTCTTCCGGTCTGACAAATGGAATAATAGAAGAAAA
  GGACATACATGAAGAATTGCCAAAACGGAAAGAAAGGAAGCAGGAGGAGAGGGAGGATGACAAAGACAGT
  GACCAGGAAACTGTTGATGAAGAAGTTGATGAAAACAGCTCAGGCATGTTTGCTGCAGAAGAGACAGGGG
  AGGCACTGTCTGAGGAGACTACAGCAGGTGAACAGTCTACAAGGTCTTTTATCTTGGATAAAATCATTGA
  AGAGGATGATGCTTATGACTTCAGTACAGATTATGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200066 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MKRPKLKKASKRMTCHKRYKIQKKVREHHRKLRKEAKKQGHKKPRKDPGVPNSAPFKEALLREAELRKQR
  LEELKQQQKLDRQKELEKKRKLETNPDIKPSNVEPMEKEFGLCKTENKAKSGKQNSKKLYCQELKKVIEA
  SDVVLEVLDARDPLGCRCPQVEEAIVQSGQKKLVLILNKSDLVPKENLESWLNYLKKELPTVVFRASTKP
  KDKGKITKRVKAKKNAAPFRSEVCFGKEGLWKLLGGFQETCSKAIRVGVIGFPNVGKSSIINSLKQEQMC
  NVGVSMGLTRSMQVVPLDKQITIIDSPSFIVSPLNSSSALALRSPASIEVVKPMEAASAILSQADARQVV
  LKYTVPGYRNSLEFFTMLAQRRGMHQKGGIPNVEGAAKLLWSEWTGASLAYYCHPPTSWTPPPYFNESIV
  VDMKSGFNLEELEKNNAQSIRAIKGPHLANSILFQSSGLTNGIIEEKDIHEELPKRKERKQEEREDDKDS
  DQETVDEEVDENSSGMFAAEETGEALSEETTAGEQSTRSFILDKIIEEDDAYDFSTDYV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014366
• ORF Size: 1647 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014366.2, NM_014366.3, NM_014366.4, NP_055181.3
• RefSeq Size: 2048 bp
• RefSeq ORF: 1650 bp
• Locus ID: 26354
• Domains: MMR_HSR1
• Protein Families: ES Cell Differentiation/IPS, Stem cell - Pluripotency
• MW: 62 kDa
• Gene Summary: The protein encoded by this gene may interact with p53 and may be involved in tumorigenesis. The encoded protein also appears to be important for stem cell proliferation. This protein is found in both the nucleus and nucleolus. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Nov 2010]