ZNF274 (NM_016324) Human Tagged ORF Clone

CAT#: RC200209

ZNF274 (Myc-DDK-tagged)-Human zinc finger protein 274 (ZNF274), transcript variant ZNF274b

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZNF274
• Synonyms: HFB101; ZF2; ZKSCAN19; ZSCAN51
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200209 representing NM_016324
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACATTGAGGTTGTTGAGGTCCTCACACTGAACCAGGAGGTGGCTGGTCCCCGGAATGCCCAGATCC
  AGGCCCTATATGCTGAAGATGGAAGCCTGAGTGCAGATGCCCCCAGTGAGCAGGTCCAACAGCAGGGCAA
  GCATCCAGGTGACCCTGAGGCCGCGCGCCAGAGGTTCCGGCAGTTCCGTTATAAGGACATGACAGGTCCC
  CGGGAGGCCCTGGACCAGCTCCGAGAGCTGTGTCACCAGTGGCTACAGCCTAAGGCACGCTCCAAGGAGC
  AGATCCTGGAGCTGCTGGTGCTGGAGCAGTTCCTAGGTGCACTGCCTGTGAAGCTCCGGACATGGGTGGA
  ATCGCAGCACCCAGAGAACTGCCAAGAGGTGGTGGCCCTGGTAGAGGGTGTGACCTGGATGTCTGAGGAG
  GAAGTACTTCCTGCAGGACAACCTGCCGAGGGCACCACCTGCTGCCTCGAGGTCACTGCCCAGCAGGAGG
  AGAAGCAGGAGGATGCAGCCATCTGCCCAGTGACAGTGCTCCCTGAGGAGCCAGTGACCTTCCAGGATGT
  GGCTGTGGACTTCAGCCGGGAGGAGTGGGGGCTGCTGGGCCCGACACAGAGGACCGAGTACCGCGATGTG
  ATGCTGGAGACCTTTGGGCACCTGGTCTCTGTGGGGTGGGAGACTACACTGGAAAATAAAGAGTTAGCTC
  CAAATTCTGACATTCCTGAGGAAGAACCAGCCCCCAGCCTGAAAGTACAAGAATCCTCAAGGGATTGTGC
  CTTGTCCTCTACATTAGAAGATACCTTGCAGGGTGGGGTCCAGGAAGTCCAAGACACAGTGTTGAAGCAG
  ATGGAGTCTGCTCAGGAAAAAGACCTTCCTCAGAAGAAGCACTTTGACAACCGTGAGTCCCAGGCAAACA
  GTGGTGCTCTTGACACAAACCAAGTTTCGCTCCAGAAAATTGACAACCCTGAGTCCCAGGCAAACAGTGG
  CGCTCTTGACACAAACCAAGTTTTGCTCCACAAAATTCCTCCTAGAAAACGATTGCGCAAACGTGACTCA
  CAAGTTAAAAGTATGAAACATAATTCACGTGTAAAAATTCATCAGAAGAGCTGTGAAAGGCAAAAGGCCA
  AGGAAGGCAATGGTTGTAGGAAAACCTTCAGTCGGAGTACTAAACAGATTACGTTTATAAGAATTCACAA
  GGGGAGCCAAGTTTGCCGATGCAGTGAATGTGGTAAAATATTCCGGAACCCAAGATACTTTTCTGTGCAT
  AAGAAAATCCATACCGGAGAGAGGCCCTATGTGTGTCAAGACTGTGGGAAAGGATTTGTTCAGAGCTCTT
  CCCTCACACAGCATCAGAGAGTTCATTCTGGAGAGAGACCATTTGAATGTCAGGAGTGTGGGAGGACCTT
  CAATGATCGCTCAGCCATCTCCCAGCACCTGAGGACTCACACTGGCGCTAAGCCCTACAAGTGTCAGGAC
  TGTGGAAAAGCCTTCCGCCAGAGCTCCCACCTCATCAGACATCAGAGGACTCACACCGGGGAGCGCCCAT
  ATGCATGCAACAAATGTGGAAAGGCCTTCACCCAGAGCTCACACCTTATTGGGCACCAGAGAACCCACAA
  TAGGACAAAGCGAAAGAAGAAACAGCCTACCTCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200209 representing NM_016324
  Red=Cloning site Green=Tags(s)
  
  MNIEVVEVLTLNQEVAGPRNAQIQALYAEDGSLSADAPSEQVQQQGKHPGDPEAARQRFRQFRYKDMTGP
  REALDQLRELCHQWLQPKARSKEQILELLVLEQFLGALPVKLRTWVESQHPENCQEVVALVEGVTWMSEE
  EVLPAGQPAEGTTCCLEVTAQQEEKQEDAAICPVTVLPEEPVTFQDVAVDFSREEWGLLGPTQRTEYRDV
  MLETFGHLVSVGWETTLENKELAPNSDIPEEEPAPSLKVQESSRDCALSSTLEDTLQGGVQEVQDTVLKQ
  MESAQEKDLPQKKHFDNRESQANSGALDTNQVSLQKIDNPESQANSGALDTNQVLLHKIPPRKRLRKRDS
  QVKSMKHNSRVKIHQKSCERQKAKEGNGCRKTFSRSTKQITFIRIHKGSQVCRCSECGKIFRNPRYFSVH
  KKIHTGERPYVCQDCGKGFVQSSSLTQHQRVHSGERPFECQECGRTFNDRSAISQHLRTHTGAKPYKCQD
  CGKAFRQSSHLIRHQRTHTGERPYACNKCGKAFTQSSHLIGHQRTHNRTKRKKKQPTS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016324
• ORF Size: 1644 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_016324.1, NM_016324.2, NM_016324.3, NP_057408.2
• RefSeq Size: 2589
• RefSeq ORF: 1647
• Locus ID: 10782
• Domains: KRAB, LER, zf-C2H2
• Protein Families: Transcription Factors
• Protein Pathways: Neurotrophin signaling pathway
• MW: 62.1 kDa
• Gene Summary: This gene encodes a zinc finger protein containing five C2H2-type zinc finger domains, one or two Kruppel-associated box A (KRAB A) domains, and a leucine-rich domain. The encoded protein has been suggested to be a transcriptional repressor. It localizes predominantly to the nucleolus. Alternatively spliced transcript variants encoding different isoforms exist. These variants utilize alternative polyadenylation signals. [provided by RefSeq, Jul 2008]