MCAK (KIF2C) (NM_006845) Human Tagged ORF Clone

CAT#: RC200254

KIF2C (Myc-DDK-tagged)-Human kinesin family member 2C (KIF2C)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY human KIF2C (RC200254, Middle lane) cDNA or pCMV6-ENTRY mouse KIF2C (MR210280, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KIF2C. (TA503320, 1:500)

• 

  Western blot validation of overexpression lysate (Cat# LY402046) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200254 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified KIF2C protein (Cat# TP300254). The protein was produced from HEK293T cells transfected with KIF2C cDNA clone (Cat# RC200254) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: KIF2C
• Synonyms: CT139; KNSL6; MCAK
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200254 representing NM_006845.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGCCATGGACTCGTCGCTTCAGGCCCGCCTGTTTCCCGGTCTCGCTATCAAGATCCAACGCAGTAAT
  GGTTTAATTCACAGTGCCAATGTAAGGACTGTGAACTTGGAGAAATCCTGTGTTTCAGTGGAATGGGCA
  GAAGGAGGTGCCACAAAGGGCAAAGAGATTGATTTTGATGATGTGGCTGCAATAAACCCAGAACTCTTA
  CAGCTTCTTCCCTTACATCCGAAGGACAATCTGCCCTTGCAGGAAAATGTAACAATCCAGAAACAAAAA
  CGGAGATCCGTCAACTCCAAAATTCCTGCTCCAAAAGAAAGTCTTCGAAGCCGCTCCACTCGCATGTCC
  ACTGTCTCAGAGCTTCGCATCACGGCTCAGGAGAATGACATGGAGGTGGAGCTGCCTGCAGCTGCAAAC
  TCCCGCAAGCAGTTTTCAGTTCCTCCTGCCCCCACTAGGCCTTCCTGCCCTGCAGTGGCTGAAATACCA
  TTGAGGATGGTCAGCGAGGAGATGGAAGAGCAAGTCCATTCCATCCGAGGCAGCTCTTCTGCAAACCCT
  GTGAACTCAGTTCGGAGGAAATCATGTCTTGTGAAGGAAGTGGAAAAAATGAAGAACAAGCGAGAAGAG
  AAGAAGGCCCAGAACTCTGAAATGAGAATGAAGAGAGCTCAGGAGTATGACAGTAGTTTTCCAAACTGG
  GAATTTGCCCGAATGATTAAAGAATTTCGGGCTACTTTGGAATGTCATCCACTTACTATGACTGATCCT
  ATCGAAGAGCACAGAATATGTGTCTGTGTTAGGAAACGCCCACTGAATAAGCAAGAATTGGCCAAGAAA
  GAAATTGATGTGATTTCCATTCCTAGCAAGTGTCTCCTCTTGGTACATGAACCCAAGTTGAAAGTGGAC
  TTAACAAAGTATCTGGAGAACCAAGCATTCTGCTTTGACTTTGCATTTGATGAAACAGCTTCGAATGAA
  GTTGTCTACAGGTTCACAGCAAGGCCACTGGTACAGACAATCTTTGAAGGTGGAAAAGCAACTTGTTTT
  GCATATGGCCAGACAGGAAGTGGCAAGACACATACTATGGGCGGAGACCTCTCTGGGAAAGCCCAGAAT
  GCATCCAAAGGGATCTATGCCATGGCCTCCCGGGACGTCTTCCTCCTGAAGAATCAACCCTGCTACCGG
  AAGTTGGGCCTGGAAGTCTATGTGACATTCTTCGAGATCTACAATGGGAAGCTGTTTGACCTGCTCAAC
  AAGAAGGCCAAGCTGCGCGTGCTGGAGGACGGCAAGCAACAGGTGCAAGTGGTGGGGCTGCAGGAGCAT
  CTGGTTAACTCTGCTGATGATGTCATCAAGATGATCGACATGGGCAGCGCCTGCAGAACCTCTGGGCAG
  ACATTTGCCAACTCCAATTCCTCCCGCTCCCACGCGTGCTTCCAAATTATTCTTCGAGCTAAAGGGAGA
  ATGCATGGCAAGTTCTCTTTGGTAGATCTGGCAGGGAATGAGCGAGGCGCGGACACTTCCAGTGCTGAC
  CGGCAGACCCGCATGGAGGGCGCAGAAATCAACAAGAGTCTCTTAGCCCTGAAGGAGTGCATCAGGGCC
  CTGGGACAGAACAAGGCTCACACCCCGTTCCGTGAGAGCAAGCTGACACAGGTGCTGAGGGACTCCTTC
  ATTGGGGAGAACTCTAGGACTTGCATGATTGCCACGATCTCACCAGGCATAAGCTCCTGTGAATATACT
  TTAAACACCCTGAGATATGCAGACAGGGTCAAGGAGCTGAGCCCCCACAGTGGGCCCAGTGGAGAGCAG
  TTGATTCAAATGGAAACAGAAGAGATGGAAGCCTGCTCTAACGGGGCGCTGATTCCAGGCAATTTATCC
  AAGGAAGAGGAGGAACTGTCTTCCCAGATGTCCAGCTTTAACGAAGCCATGACTCAGATCAGGGAGCTG
  GAGGAGAAGGCTATGGAAGAGCTCAAGGAGATCATACAGCAAGGACCAGACTGGCTTGAGCTCTCTGAG
  ATGACCGAGCAGCCAGACTATGACCTGGAGACCTTTGTGAACAAAGCGGAATCTGCTCTGGCCCAGCAA
  GCCAAGCATTTCTCAGCCCTGCCAGATGTCATCAAGGCCTTGCGCCTGGCCATGCAGCTGGAAGAGCAG
  GCTAGCAGACAAATAAGCAGCAAGAAACGGCCCCAG
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGAT
  ATCCTGGATTACAAGGATGACGACGATAAGGTTTAA
  
  Protein Sequence

  >Peptide sequence encoded by RC200254
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MAMDSSLQARLFPGLAIKIQRSNGLIHSANVRTVNLEKSCVSVEWAEGGATKGKEIDFDDVAAINPELL
  QLLPLHPKDNLPLQENVTIQKQKRRSVNSKIPAPKESLRSRSTRMSTVSELRITAQENDMEVELPAAAN
  SRKQFSVPPAPTRPSCPAVAEIPLRMVSEEMEEQVHSIRGSSSANPVNSVRRKSCLVKEVEKMKNKREE
  KKAQNSEMRMKRAQEYDSSFPNWEFARMIKEFRATLECHPLTMTDPIEEHRICVCVRKRPLNKQELAKK
  EIDVISIPSKCLLLVHEPKLKVDLTKYLENQAFCFDFAFDETASNEVVYRFTARPLVQTIFEGGKATCF
  AYGQTGSGKTHTMGGDLSGKAQNASKGIYAMASRDVFLLKNQPCYRKLGLEVYVTFFEIYNGKLFDLLN
  KKAKLRVLEDGKQQVQVVGLQEHLVNSADDVIKMIDMGSACRTSGQTFANSNSSRSHACFQIILRAKGR
  MHGKFSLVDLAGNERGADTSSADRQTRMEGAEINKSLLALKECIRALGQNKAHTPFRESKLTQVLRDSF
  IGENSRTCMIATISPGISSCEYTLNTLRYADRVKELSPHSGPSGEQLIQMETEEMEACSNGALIPGNLS
  KEEEELSSQMSSFNEAMTQIRELEEKAMEELKEIIQQGPDWLELSEMTEQPDYDLETFVNKAESALAQQ
  AKHFSALPDVIKALRLAMQLEEQASRQISSKKRPQ
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
  
  Recombinant protein using RC200254 also available, TP300254M
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006845
• ORF Size: 2175 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq Size: 2896 bp
• RefSeq ORF: 2178 bp
• Locus ID: 11004
• UniProt ID: Q99661
• Cytogenetics: 1p34.1
• Domains: kinesin
• Protein Families: Druggable Genome
• MW: 81.3 kDa
• Gene Summary: This gene encodes a kinesin-like protein that functions as a microtubule-dependent molecular motor. The encoded protein can depolymerize microtubules at the plus end, thereby promoting mitotic chromosome segregation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]