AKAP1 (NM_003488) Human Tagged ORF Clone

CAT#: RC200506

AKAP1 (Myc-DDK-tagged)-Human A kinase (PRKA) anchor protein 1 (AKAP1), nuclear gene encoding mitochondrial protein

Product Images

Western blot validation of overexpression lysate (Cat# LY418643) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200506 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: AKAP1
• Synonyms: AKAP; AKAP84; AKAP121; AKAP149; D-AKAP1; PPP1R43; PRKA1; SAKAP84; TDRD17
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200506 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAATCCAGTTCCGTTCGCTCTTCCCCTTGGCATTGCCTGGGATGCTGGCGCTCCTCGGCTGGTGGT
  GGTTTTTCTCTCGTAAAAAAGGCCATGTCAGCAGCCATGATGAGCAGCAGGTGGAGGCTGGTGCTGTGCA
  GCTGAGGGCTGACCCTGCCATCAAGGAACCTCTCCCCGTGGAAGACGTCTGTCCCAAAGTAGTGTCCACA
  CCCCCCAGTGTCACAGAGCCTCCAGAAAAGGAACTGTCCACCGTGAGCAAGCTGCCTGCAGAGCCCCCAG
  CATTGCTCCAGACACACCCACCTTGCCGAAGATCAGAGTCCTCGGGCATTCTTCCTAACACCACAGACAT
  GAGATTGCGACCAGGAACACGCAGAGATGACAGTACAAAGCTGGAGCTAGCCCTGACAGGTGGTGAAGCC
  AAATCGATTCCTCTAGAGTGCCCCCTTTCATCCCCAAAGGGTGTACTATTCTCCAGCAAATCAGCTGAGG
  TGTGTAAGCAAGATTCCCCCTTCAGCAGGGTGCCAAGGAAGGTCCAGCCAGGCTACCCCGTAGTCCCCGC
  AGAGAAGCGTAGCTCTGGGGAGAGGGCAAGAGAGACAGGTGGGGCCGAAGGGACTGGTGATGCCGTGTTG
  GGGGAAAAGGTGCTTGAAGAAGCTCTGTTGTCTCGGGAGCATGTCTTGGAATTGGAGAACAGCAAGGGCC
  CCAGCCTGGCCTCTTTAGAGGGGGAAGAAGATAAGGGGAAGAGCAGCTCATCCCAGGTGGTGGGGCCAGT
  GCAGGAGGAAGAGTATGTAGCAGAGAAGTTGCCAAGTAGGTTCATCGAGTCGGCTCACACAGAGCTGGCA
  AAGGACGATGCGGCGCCAGCACCCCCAGTCGCAGACGCCAAAGCCCAGGATAGAGGTGTCGAGGGAGAAC
  TGGGCAATGAGGAGAGCTTGGATAGAAATGAGGAGGGCTTGGATAGAAATGAGGAGGGCTTGGATAGAAA
  TGAGGAGAGCTTGGATAGAAATGAGGAGGGCTTGGATAGAAATGAGGAGATTAAGCGGGCTGCCTTCCAG
  ATAATCTCCCAAGTGATCTCAGAAGCAACCGAACAGGTGCTGGCCACCACGGTTGGCAAGGTTGCAGGTC
  GTGTGTGTCAGGCCAGTCAGCTCCAAGGGCAGAAGGAAGAGAGCTGTGTCCCAGTTCACCAGAAAACTGT
  CTTGGGCCCAGACACTGCGGAGCCTGCCACAGCAGAGGCAGCTGTTGCCCCGCCGGATGCTGGCCTCCCC
  TTGCCAGGCCTACCAGCAGAGGGCTCACCACCACCAAAGACCTACGTGAGCTGCCTGAAGAGCCTTCTGT
  CCAGCCCCACCAAGGACAGTAAGCCAAATATCTCTGCACACCACATCTCCCTGGCCTCCTGCCTGGCACT
  GACCACCCCCAGTGAAGAGTTGCCGGACCGGGCAGGCATCCTGGTGGAAGATGCCACCTGTGTCACCTGC
  ATGTCAGACAGCAGCCAAAGTGTCCCTTTGGTGGCTTCTCCAGGACACTGCTCAGATTCTTTCAGCACTT
  CAGGGCTTGAAGACTCTTGCACAGAGACCAGCTCGAGCCCCAGGGACAAGGCCATCACCCCGCCACTGCC
  AGAAAGTACTGTGCCCTTCAGCAATGGGGTGCTGAAGGGGGAGTTGTCAGACTTGGGGGCTGAGGATGGA
  TGGACCATGGATGCGGAAGCAGATCATTCAGGAGGTTCTGACAGGAACAGCATGGATTCCGTGGATAGCT
  GTTGCAGTCTCAAGAAGACTGAGAGCTTCCAAAATGCCCAGGCAGGCTCCAACCCTAAGAAGGTCGACCT
  CATCATCTGGGAGATCGAGGTGCCAAAGCACTTAGTCGGTCGGCTAATTGGCAAGCAGGGGCGCTATGTG
  AGTTTTCTGAAGCAAACATCTGGTGCCAAGATCTACATTTCAACCCTGCCTTACACCCAGAGCGTCCAGA
  TCTGCCACATAGAAGGCTCTCAACATCATGTAGACAAAGCGCTGAACTTGATTGGGAAGAAGTTCAAAGA
  GCTGAACCTCACCAATATCTACGCTCCCCCATTGCCTTCACTGGCACTGCCTTCTCTGCCGATGACATCC
  TGGCTCATGCTGCCTGATGGCATCACCGTGGAGGTCATTGTGGTCAACCAGGTCAATGCCGGGCACCTGT
  TCGTGCAGCAGCACACACACCCTACCTTCCACGCGCTGCGCAGCCTCGACCAGCAGATGTACCTCTGTTA
  CTCTCAGCCTGGAATCCCCACCTTGCCCACCCCAGTGGAAATAACGGTCATCTGTGCCGCCCCTGGTGCG
  GACGGGGCCTGGTGGCGAGCCCAAGTGGTTGCCTCCTACGAGGAGACCAACGAAGTGGAGATTCGATACG
  TGCACTACGGCGGATATAAGAGGGTGAAAGTAGACGTGCTCCGGCAAATCAGGTCTGACTTTGTCACCCT
  GCCGTTTCAGGGAGCAGAAGTCCTTCTGGACAGTGTGATGCCCCTGTCAGACGATGACCAGTTTTCACCG
  GAAGCAGATGCCGCCATGAGCGAGATGACGGGGAATACAGCACTGCTTGCTCAGGTGACAAGTTACAGTC
  CAACTGGTCTTCCTCTGATTCAGCTGTGGAGTGTGGTTGGAGATGAAGTGGTGTTGATAAACCGGTCCCT
  GGTGGAGCGAGGCCTTGCCCAGTGGGTAGACAGCTACTACACAAGCCTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200506 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAIQFRSLFPLALPGMLALLGWWWFFSRKKGHVSSHDEQQVEAGAVQLRADPAIKEPLPVEDVCPKVVST
  PPSVTEPPEKELSTVSKLPAEPPALLQTHPPCRRSESSGILPNTTDMRLRPGTRRDDSTKLELALTGGEA
  KSIPLECPLSSPKGVLFSSKSAEVCKQDSPFSRVPRKVQPGYPVVPAEKRSSGERARETGGAEGTGDAVL
  GEKVLEEALLSREHVLELENSKGPSLASLEGEEDKGKSSSSQVVGPVQEEEYVAEKLPSRFIESAHTELA
  KDDAAPAPPVADAKAQDRGVEGELGNEESLDRNEEGLDRNEEGLDRNEESLDRNEEGLDRNEEIKRAAFQ
  IISQVISEATEQVLATTVGKVAGRVCQASQLQGQKEESCVPVHQKTVLGPDTAEPATAEAAVAPPDAGLP
  LPGLPAEGSPPPKTYVSCLKSLLSSPTKDSKPNISAHHISLASCLALTTPSEELPDRAGILVEDATCVTC
  MSDSSQSVPLVASPGHCSDSFSTSGLEDSCTETSSSPRDKAITPPLPESTVPFSNGVLKGELSDLGAEDG
  WTMDAEADHSGGSDRNSMDSVDSCCSLKKTESFQNAQAGSNPKKVDLIIWEIEVPKHLVGRLIGKQGRYV
  SFLKQTSGAKIYISTLPYTQSVQICHIEGSQHHVDKALNLIGKKFKELNLTNIYAPPLPSLALPSLPMTS
  WLMLPDGITVEVIVVNQVNAGHLFVQQHTHPTFHALRSLDQQMYLCYSQPGIPTLPTPVEITVICAAPGA
  DGAWWRAQVVASYEETNEVEIRYVHYGGYKRVKVDVLRQIRSDFVTLPFQGAEVLLDSVMPLSDDDQFSP
  EADAAMSEMTGNTALLAQVTSYSPTGLPLIQLWSVVGDEVVLINRSLVERGLAQWVDSYYTSL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003488
• ORF Size: 2709 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003488.1, NM_003488.2, NM_003488.3, NP_003479.1
• RefSeq Size: 3968 bp
• RefSeq ORF: 2712 bp
• Locus ID: 8165
• Cytogenetics: 17q22
• Domains: TUDOR, KH, TUDOR
• Protein Families: Druggable Genome, Transmembrane
• MW: 97.4 kDa
• Gene Summary: The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein binds to type I and type II regulatory subunits of PKA and anchors them to the mitochondrion. This protein is speculated to be involved in the cAMP-dependent signal transduction pathway and in directing RNA to a specific cellular compartment. [provided by RefSeq, Jul 2008]